Q8FX16 (NOSZ_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nitrous-oxide reductase
EC=1.7.2.4

Alternative name(s):
N(2)OR
N2O reductase

Gene names

Name:	nosZ
Ordered Locus Names:	BRA0275, BS1330_II0272

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	639 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Nitrous-oxide reductase is part of a bacterial respiratory system which is activated under anaerobic conditions in the presence of nitrate or nitrous oxide By similarity. HAMAP MF_00716
Catalytic activity	Nitrogen + H2O + 2 cytochrome c = nitrous oxide + 2 reduced cytochrome c. HAMAP MF_00716
Cofactor	Binds 2 calcium ions per subunit By similarity. HAMAP MF_00716 Binds 6 copper ions per subunit. Each subunit contains 2 copper centers; Cu(A) (binuclear) and Cu(Z) (tetranuclear). Cu(Z) is thought to be the site of nitrous oxide reduction By similarity.
Pathway	Nitrogen metabolism; nitrate reduction (denitrification); dinitrogen from nitrate: step 4/4. HAMAP MF_00716
Subunit structure	Homodimer By similarity. HAMAP MF_00716
Subcellular location	Periplasm By similarity HAMAP MF_00716.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven. HAMAP MF_00716
Sequence similarities	Belongs to the nosZ family. In the C-terminal section; belongs to the cytochrome c oxidase subunit 2 family.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Calcium Copper Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	membrane Inferred from electronic annotation. Source: InterPro periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	calcium ion binding Inferred from electronic annotation. Source: InterPro copper ion binding Inferred from electronic annotation. Source: InterPro cytochrome-c oxidase activity Inferred from electronic annotation. Source: InterPro nitrous-oxide reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 44	44	Tat-type signal Potential
	Chain	45 – 639	595	Nitrous-oxide reductase HAMAP MF_00716		PRO_0000019826
Regions
	Region	541 – 639	99	COX2-like HAMAP MF_00716
Sites
	Metal binding	136	1	Copper Z2 By similarity
	Metal binding	137	1	Copper Z3 By similarity
	Metal binding	185	1	Copper Z2 By similarity
	Metal binding	262	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	265	1	Calcium 2 By similarity
	Metal binding	273	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	279	1	Calcium 2 By similarity
	Metal binding	324	1	Calcium 2 By similarity
	Metal binding	326	1	Copper Z1 By similarity
	Metal binding	381	1	Copper Z1 By similarity
	Metal binding	432	1	Copper Z3 By similarity
	Metal binding	453	1	Calcium 1; via carbonyl oxygen By similarity
	Metal binding	468	1	Calcium 1 By similarity
	Metal binding	493	1	Copper Z4 By similarity
	Metal binding	582	1	Copper A1 By similarity
	Metal binding	617	1	Copper A1 By similarity
	Metal binding	617	1	Copper A2 By similarity
	Metal binding	619	1	Copper A2; via carbonyl oxygen By similarity
	Metal binding	621	1	Copper A1 By similarity
	Metal binding	621	1	Copper A2 By similarity
	Metal binding	625	1	Copper A2 By similarity
	Metal binding	628	1	Copper A1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FX16 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: DFB2E8DBB372B057	FASTA	639	71,071
	10 20 30 40 50 60 MTEETRKSQL SRRQLLGTSA FVAAAGASGL GGALLAGSSE TALAAGAAKG GMSPEVKPGE 70 80 90 100 110 120 LDEYYVFFSS GQCGEVRIVG LPSMRELMRI PVFNRDSATG WGLTNESRKV LTEGLLPQDR 130 140 150 160 170 180 EFLKDRGDIF LNGDLHHPHP SFTDGTYDGR YLYANDKANT RVCRIRLDVM KCDKIIQLPN 190 200 210 220 230 240 QHTVHGLRVQ KYPKTGYVFC NGEDRVPIPN DGSHLNDVKQ YHAIFTAVDG ETMKVAWQVM 250 260 270 280 290 300 VDGNLDNVDC DYQGKYAFST CYNSEEGTTL AEMMSSEQDW IVVFNLKRIE EAVANGDFKE 310 320 330 340 350 360 MNGVPVIDGR HGSKYTRYIP VPNSPHGINT APDGIHVVAN GKLSPTVTVF DVRKFDDLFD 370 380 390 400 410 420 DKIKPRDAVV AEPELGLGPL HTAYDDKGNA YTTLFIDSQI CKWNIEDAKR AFKGEKVDPI 430 440 450 460 470 480 RQKLDVHYQP GHNHSSMGQT KEVDGKWLIS LNKFSKDRYL NVGPLKPEND QLIDISGDKM 490 500 510 520 530 540 VIVHDGPSFA EPHDATIVHR SKINPVSFWS REDPFFADAV AQAKADGLDL MEANEVVRDG 550 560 570 580 590 600 NKVRVYMTSS APAFGLTEFT VQQDDEVTVY VTNIDEIEDL THGFAIVNYG VNMEVAPQAT 610 620 630 ASVTFKASKP GVWWYYCSWF CHAMHMEMQG RMLVEPKKA

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014292 Genomic DNA. Translation: AAN33476.1. CP002998 Genomic DNA. Translation: AEM19754.1.
RefSeq	NP_699471.1. NC_004311.2.
3D structure databases
ProteinModelPortal	Q8FX16.
SMR	Q8FX16. Positions 56-637.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1164712.
GenomeReviews	Gene locus BRA0275 in contig AE014292_GR.
KEGG	bms:BRA0275.
PATRIC	17793347. VBIBruSui107850_2492.
TIGR	BRA0275.
Phylogenomic databases
HOGENOM	HBG308574.
OMA	KLVHDGP.
PhylomeDB	Q8FX16.
ProtClustDB	PRK02888.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_A0275-MONOMER.
Family and domain databases
HAMAP	MF_00716. NosZ. [Tree]
InterPro	IPR008972. Cupredoxin. IPR002429. Cyt_c_oxidase_su2_C. IPR011045. N2O_reductase_N. IPR023644. NO_Rdtase. IPR006311. TAT_signal. IPR015943. WD40/YVTN_repeat-like_dom. [Graphical view]
Gene3D	G3DSA:2.60.40.420. Cupredoxin. 1 hit. G3DSA:2.130.10.10. WD40/YVTN_repeat-like. 1 hit.
KO	K00376.
Pfam	PF00116. COX2. 1 hit. [Graphical view]
SUPFAM	SSF49503. Cupredoxin. 1 hit. SSF50974. N2O_reductase_N. 1 hit.
PROSITE	PS00078. COX2. False negative. PS50857. COX2_CUA. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NOSZ_BRUSU

Accession

Primary (citable) accession number: Q8FX16
Secondary accession number(s): G0KFB0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 3, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program