Q8FWV9 (BEPG_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Efflux pump membrane transporter BepG

Gene names

Name:	bepG
Ordered Locus Names:	BRA0334, BS1330_II0331

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	1074 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE. Ref.3
Subunit structure	Probably part of a tripartite efflux pump, which is composed of an outer membrane efflux protein, an inner membrane protein and a protein that expands the periplasmic space. Could form a tripartite pump with BepC and BepF. Ref.3
Subcellular location	Cell inner membrane; Multi-pass membrane protein Potential.
Induction	Induced in the absence of BepDE. Ref.3
Disruption phenotype	Shows no differences in the resistance profile to different dyes, detergents and antimicrobials. Ref.3
Sequence similarities	Belongs to the AcrB/AcrD/AcrF (TC 2.A.6) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome
Gene Ontology (GO)
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	transporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1074	1074	Efflux pump membrane transporter BepG		PRO_0000390651
Regions
	Transmembrane	13 – 33	21	Helical; Potential
	Transmembrane	342 – 362	21	Helical; Potential
	Transmembrane	368 – 388	21	Helical; Potential
	Transmembrane	395 – 415	21	Helical; Potential
	Transmembrane	444 – 464	21	Helical; Potential
	Transmembrane	478 – 498	21	Helical; Potential
	Transmembrane	540 – 560	21	Helical; Potential
	Transmembrane	876 – 896	21	Helical; Potential
	Transmembrane	898 – 918	21	Helical; Potential
	Transmembrane	930 – 950	21	Helical; Potential
	Transmembrane	975 – 995	21	Helical; Potential
	Transmembrane	1007 – 1027	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8FWV9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 79F0B440F8A96438	FASTA	1,074	114,902
	10 20 30 40 50 60 MLSSVFINRP RLAIVIAIVI TLAGLIAVTR IPVAQFPDIV PPQVSVTATY PGASAETVEA 70 80 90 100 110 120 AIAQPIEAQV NGVDDMIYMS STSGNNGTYT LTVTFKVGSD PNLNTVNVQN RVRLAEANLP 130 140 150 160 170 180 QEVTRLGVTV KKQSSSFLQI ITLLSPDSRY DELFLNNYGV INVVDRLARV PGVGQAQSFG 190 200 210 220 230 240 TFNYSMRIWF NTDALTSLNL TPNDIVNAIS SQNVQAAVGR LGAPPMTDQQ QIQLTLTTQG 250 260 270 280 290 300 RLTDAKQFEN IIIRANPDGS SVRLKDVARV ELAAQSYDTI GRLNGKPASV IAVYQAPGSN 310 320 330 340 350 360 AVAAAEGVRN VMEQLKQSFP AGLDYKITYD TTVFVSSTIH EVIKTLLEAF VLVVVVVFIF 370 380 390 400 410 420 LGNFRATLIP TLAVPVSLIG TFAVLLVLGF SANTISLFAM ILAIGIVVDD AIVVVENVER 430 440 450 460 470 480 VMAETGLPPK EAAKQAMQEI TAPIIAITLV LLSVFVPVAF IPGITGALYA QFALTVSVAM 490 500 510 520 530 540 LISAINALTL SPALCGVFLK PHQGRKKSLY GRTMDKLSSG IEKISDGYAH IVRRLVRMAF 550 560 570 580 590 600 LSIVLVAGLG AGAYFLNTIV PTGFLPEEDQ GLFFVQVNLP PAASQSRTAA VVSEIEADIT 610 620 630 640 650 660 KMAGVADVTS VTGFSFIDGL AVSNAGLMIV TLKPLEERLK DNITVFDVIA EVNRRTAAIP 670 680 690 700 710 720 SAVAITMNLP PILGLGSSGG FQYQLEDQEG QSPQQLASVA QGLVMAANQN PKLSRVFTTF 730 740 750 760 770 780 ATDTPQLNLN IDRQKALSLG VSPNNIIQAL QSTLGGYFVN NFNTLGRTWQ VIIQGEQQDR 790 800 810 820 830 840 KTVEDIYRIN VRSSHGDMVP LRSLVSVEER LGPLYITRYN NYRSASIQGN AAPGVSSGEA 850 860 870 880 890 900 LAAMAQVSKT TLPSGYGYEW TGTALQELQA AGQTSMILAL AVLFAYLFLV ALYESWTIPV 910 920 930 940 950 960 GVLLSVTAGL AGAMLALWIT GLSNDIYAQI GIVVLIALAS KNGILIVEFA KERREEGVPL 970 980 990 1000 1010 1020 EQAAIIGARQ RFRPVMMTSF AFILGLVPLV IAVGAAAASR RAVGTSVFGG MIAASAVGIF 1030 1040 1050 1060 1070 LIPMLYVVLE RVREWGHARI LRKPLYEEEK QEKADGDASG PTVPPTQPED RGLS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[2]	"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.
[3]	"Interplay between two RND systems mediating antimicrobial resistance in Brucella suis." Martin F.A., Posadas D.M., Carrica M.C., Cravero S.L., O'Callaghan D., Zorreguieta A. J. Bacteriol. 191:2530-2540(2009) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION IN RESISTANCE, SUBUNIT, INDUCTION, DISRUPTION PHENOTYPE. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014292 Genomic DNA. Translation: AAN33534.1. CP002998 Genomic DNA. Translation: AEM19813.1.
RefSeq	NP_699529.1. NC_004311.2. YP_005614039.1. NC_017250.1.
3D structure databases
HSSP	HSSP built from PDB template 1IWG based on UniProtKB P31224.
ProteinModelPortal	Q8FWV9.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BRA0334.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN33534; AAN33534; BRA0334. AEM19813; AEM19813; BS1330_II0331.
GeneID	1164772. 12139766.
KEGG	bms:BRA0334. bsi:BS1330_II0331.
PATRIC	17793463. VBIBruSui107850_2549.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0841.
HOGENOM	HOG000158129.
OMA	FITNYAI.
ProtClustDB	CLSK2304408.
Family and domain databases
InterPro	IPR001036. Acrflvin-R. IPR004764. HAE1. [Graphical view]
Pfam	PF00873. ACR_tran. 1 hit. [Graphical view]
PRINTS	PR00702. ACRIFLAVINRP.
TIGRFAMs	TIGR00915. 2A0602. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BEPG_BRUSU

Accession

Primary (citable) accession number: Q8FWV9
Secondary accession number(s): G0KC75

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 19, 2010
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program