Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Bifunctional enzyme NanE/NanK

Gene

nanEK

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P).Curated
Catalyzes the phosphorylation of N-acetylmannosamine (ManNAc) to ManNAc-6-P.By similarity

Catalytic activityi

N-acyl-D-glucosamine 6-phosphate = N-acyl-D-mannosamine 6-phosphate.
ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate.

Pathwayi: N-acetylneuraminate degradation

This protein is involved in step 2 and 3 of the subpathway that synthesizes D-fructose 6-phosphate from N-acetylneuraminate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Bifunctional enzyme NanE/NanK (nanEK)
  3. Bifunctional enzyme NanE/NanK (nanEK)
  4. no protein annotated in this organism
  5. no protein annotated in this organism
This subpathway is part of the pathway N-acetylneuraminate degradation, which is itself part of Amino-sugar metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-fructose 6-phosphate from N-acetylneuraminate, the pathway N-acetylneuraminate degradation and in Amino-sugar metabolism.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi246 – 253ATPSequence analysis8
Nucleotide bindingi372 – 379ATPSequence analysis8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionIsomerase, Kinase, Multifunctional enzyme, Transferase
Biological processCarbohydrate metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00629; UER00681
UPA00629; UER00682

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional enzyme NanE/NanK
Including the following 2 domains:
Putative N-acetylmannosamine-6-phosphate 2-epimerase (EC:5.1.3.9)
Alternative name(s):
ManNAc-6-P epimerase
N-acetylmannosamine kinase (EC:2.7.1.60)
Alternative name(s):
ManNAc kinase
N-acetyl-D-mannosamine kinase
Gene namesi
Name:nanEK
Ordered Locus Names:BRA0411, BS1330_II0408
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
Proteomesi
  • UP000007104 Componenti: Chromosome II

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001798241 – 525Bifunctional enzyme NanE/NanKAdd BLAST525

Structurei

3D structure databases

ProteinModelPortaliQ8FWN5
SMRiQ8FWN5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 241ManNAc-6-P epimeraseAdd BLAST241
Regioni242 – 525ManNAc kinaseAdd BLAST284

Sequence similaritiesi

In the N-terminal section; belongs to the NanE family.Curated
In the C-terminal section; belongs to the ROK (NagC/XylR) family. NanK subfamily.Curated

Phylogenomic databases

HOGENOMiHOG000289284
KOiK13967
OMAiIANCLIV

Family and domain databases

CDDicd04729 NanE, 1 hit
Gene3Di3.20.20.70, 1 hit
HAMAPiMF_01235 ManNAc6P_epimer, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR007260 NanE
IPR011060 RibuloseP-bd_barrel
IPR000600 ROK
PANTHERiPTHR36204 PTHR36204, 1 hit
PfamiView protein in Pfam
PF04131 NanE, 1 hit
PF00480 ROK, 1 hit
SUPFAMiSSF51366 SSF51366, 1 hit

Sequencei

Sequence statusi: Complete.

Q8FWN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGSPRNLCR VQGMIEEIVN RLRGKLIVSC QPVPESPFDN VASVVAYARA
60 70 80 90 100
AEASGASGLR IEGAANVAAT AQASTLPVIG LIKRDLDDSP VRITPFLEDV
110 120 130 140 150
AALCDAGAAI VAVDATDRRR PVPAAELIGE IKRRGRIAMA DISTLAEARN
160 170 180 190 200
ALAAGADIIG TTMSGYTGEG PTPKDPDLDL VAHCSRLGSF LIAEGRYNSP
210 220 230 240 250
QQAGEAIRAG ADAVVVGSAI TRPEHITGWF RDAVESAAKP SSPVLAFDIG
260 270 280 290 300
GTKTLAALVR GREILERRVM TTPASVGSES WIGAIASLSA DWQGRYQRAA
310 320 330 340 350
IAVTGRVDGE IWSSLNPETL AIPPDYPLGR RMGAALGAPV EVINDAQAAA
360 370 380 390 400
WGENRFGAAR GRDMVFLTIS SGIGGGIVLG GRLIRGARGI AGSLGQVLVA
410 420 430 440 450
GPSGFVRLET LASGFGIAKM ALEAGHAGDA RSVFSAAAAG EGWARRILLD
460 470 480 490 500
AASQLAAAVA GLQAIVDPEC IVIGGGVGMA DGFLDMLREA LGSHSAVMRP
510 520
DIVAAELGAD AGIIGVADLA ATYFS
Length:525
Mass (Da):53,867
Last modified:March 1, 2003 - v1
Checksum:iD403CAE499AB9486
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014292 Genomic DNA Translation: AAN33608.1
CP002998 Genomic DNA Translation: AEM19887.1

Genome annotation databases

EnsemblBacteriaiAAN33608; AAN33608; BRA0411
AEM19887; AEM19887; BS1330_II0408
KEGGibms:BRA0411
bsi:BS1330_II0408

Similar proteinsi

Entry informationi

Entry nameiNANEK_BRUSU
AccessioniPrimary (citable) accession number: Q8FWN5
Secondary accession number(s): G0KCE9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 1, 2003
Last modified: March 28, 2018
This is version 88 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health