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Protein

Bifunctional enzyme NanE/NanK

Gene

nanEK

Organism
Brucella suis biovar 1 (strain 1330)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P).Curated
Catalyzes the phosphorylation of N-acetylmannosamine (ManNAc) to ManNAc-6-P.By similarity

Catalytic activityi

N-acyl-D-glucosamine 6-phosphate = N-acyl-D-mannosamine 6-phosphate.
ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate.

Pathwayi

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi246 – 2538ATPSequence Analysis
Nucleotide bindingi372 – 3798ATPSequence Analysis

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. N-acylglucosamine-6-phosphate 2-epimerase activity Source: UniProtKB-EC
  3. N-acylmannosamine kinase activity Source: UniProtKB-EC

GO - Biological processi

  1. N-acetylmannosamine metabolic process Source: InterPro
  2. N-acetylneuraminate catabolic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

UniPathwayiUPA00629; UER00681.
UPA00629; UER00682.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional enzyme NanE/NanK
Including the following 2 domains:
Putative N-acetylmannosamine-6-phosphate 2-epimerase (EC:5.1.3.9)
Alternative name(s):
ManNAc-6-P epimerase
N-acetylmannosamine kinase (EC:2.7.1.60)
Alternative name(s):
ManNAc kinase
N-acetyl-D-mannosamine kinase
Gene namesi
Name:nanEK
Ordered Locus Names:BRA0411, BS1330_II0408
OrganismiBrucella suis biovar 1 (strain 1330)
Taxonomic identifieri204722 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella
ProteomesiUP000000824: Chromosome II, UP000007104: Chromosome II

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 525525Bifunctional enzyme NanE/NanKPRO_0000179824Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi204722.BRA0411.

Structurei

3D structure databases

ProteinModelPortaliQ8FWN5.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni1 – 241241ManNAc-6-P epimeraseAdd
BLAST
Regioni242 – 525284ManNAc kinaseAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the NanE family.Curated
In the C-terminal section; belongs to the ROK (NagC/XylR) family. NanK subfamily.Curated

Phylogenomic databases

eggNOGiCOG1940.
HOGENOMiHOG000289284.
KOiK13967.
OMAiREDIANC.
OrthoDBiEOG6NSGKX.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01235. ManNAc6P_epimer.
InterProiIPR013785. Aldolase_TIM.
IPR007260. NanE.
IPR011060. RibuloseP-bd_barrel.
IPR000600. ROK.
[Graphical view]
PfamiPF04131. NanE. 1 hit.
PF00480. ROK. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FWN5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRGSPRNLCR VQGMIEEIVN RLRGKLIVSC QPVPESPFDN VASVVAYARA
60 70 80 90 100
AEASGASGLR IEGAANVAAT AQASTLPVIG LIKRDLDDSP VRITPFLEDV
110 120 130 140 150
AALCDAGAAI VAVDATDRRR PVPAAELIGE IKRRGRIAMA DISTLAEARN
160 170 180 190 200
ALAAGADIIG TTMSGYTGEG PTPKDPDLDL VAHCSRLGSF LIAEGRYNSP
210 220 230 240 250
QQAGEAIRAG ADAVVVGSAI TRPEHITGWF RDAVESAAKP SSPVLAFDIG
260 270 280 290 300
GTKTLAALVR GREILERRVM TTPASVGSES WIGAIASLSA DWQGRYQRAA
310 320 330 340 350
IAVTGRVDGE IWSSLNPETL AIPPDYPLGR RMGAALGAPV EVINDAQAAA
360 370 380 390 400
WGENRFGAAR GRDMVFLTIS SGIGGGIVLG GRLIRGARGI AGSLGQVLVA
410 420 430 440 450
GPSGFVRLET LASGFGIAKM ALEAGHAGDA RSVFSAAAAG EGWARRILLD
460 470 480 490 500
AASQLAAAVA GLQAIVDPEC IVIGGGVGMA DGFLDMLREA LGSHSAVMRP
510 520
DIVAAELGAD AGIIGVADLA ATYFS
Length:525
Mass (Da):53,867
Last modified:March 1, 2003 - v1
Checksum:iD403CAE499AB9486
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014292 Genomic DNA. Translation: AAN33608.1.
CP002998 Genomic DNA. Translation: AEM19887.1.
RefSeqiNP_699603.1. NC_004311.2.
YP_005614113.1. NC_017250.1.

Genome annotation databases

EnsemblBacteriaiAAN33608; AAN33608; BRA0411.
AEM19887; AEM19887; BS1330_II0408.
GeneIDi1164849.
12139843.
KEGGibms:BRA0411.
bsi:BS1330_II0408.
PATRICi17793616. VBIBruSui107850_2624.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AE014292 Genomic DNA. Translation: AAN33608.1.
CP002998 Genomic DNA. Translation: AEM19887.1.
RefSeqiNP_699603.1. NC_004311.2.
YP_005614113.1. NC_017250.1.

3D structure databases

ProteinModelPortaliQ8FWN5.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi204722.BRA0411.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN33608; AAN33608; BRA0411.
AEM19887; AEM19887; BS1330_II0408.
GeneIDi1164849.
12139843.
KEGGibms:BRA0411.
bsi:BS1330_II0408.
PATRICi17793616. VBIBruSui107850_2624.

Phylogenomic databases

eggNOGiCOG1940.
HOGENOMiHOG000289284.
KOiK13967.
OMAiREDIANC.
OrthoDBiEOG6NSGKX.

Enzyme and pathway databases

UniPathwayiUPA00629; UER00681.
UPA00629; UER00682.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01235. ManNAc6P_epimer.
InterProiIPR013785. Aldolase_TIM.
IPR007260. NanE.
IPR011060. RibuloseP-bd_barrel.
IPR000600. ROK.
[Graphical view]
PfamiPF04131. NanE. 1 hit.
PF00480. ROK. 1 hit.
[Graphical view]
SUPFAMiSSF51366. SSF51366. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 1330.

Entry informationi

Entry nameiNANEK_BRUSU
AccessioniPrimary (citable) accession number: Q8FWN5
Secondary accession number(s): G0KCE9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 1, 2003
Last modified: January 7, 2015
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme

Documents

  1. Brucella suis
    Brucella suis (strain 1330): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.