Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

Q8FWN5 (NANEK_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 74. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional enzyme NanE/NanK

Including the following 2 domains:

  1. Putative N-acetylmannosamine-6-phosphate 2-epimerase
    EC=5.1.3.9
    Alternative name(s):
    ManNAc-6-P epimerase
  2. N-acetylmannosamine kinase
    EC=2.7.1.60
    Alternative name(s):
    ManNAc kinase
    N-acetyl-D-mannosamine kinase
Gene names
Name:nanEK
Ordered Locus Names:BRA0411, BS1330_II0408
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length525 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Converts N-acetylmannosamine-6-phosphate (ManNAc-6-P) to N-acetylglucosamine-6-phosphate (GlcNAc-6-P) Potential. HAMAP-Rule MF_01235

Catalyzes the phosphorylation of N-acetylmannosamine (ManNAc) to ManNAc-6-P By similarity. HAMAP-Rule MF_01235

Catalytic activity

N-acyl-D-glucosamine 6-phosphate = N-acyl-D-mannosamine 6-phosphate. HAMAP-Rule MF_01235

ATP + N-acyl-D-mannosamine = ADP + N-acyl-D-mannosamine 6-phosphate. HAMAP-Rule MF_01235

Pathway

Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 2/5. HAMAP-Rule MF_01235

Amino-sugar metabolism; N-acetylneuraminate degradation; D-fructose 6-phosphate from N-acetylneuraminate: step 3/5.

Sequence similarities

In the N-terminal section; belongs to the NanE family.

In the C-terminal section; belongs to the ROK (NagC/XylR) family. NanK subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 525525Bifunctional enzyme NanE/NanK HAMAP-Rule MF_01235
PRO_0000179824

Regions

Nucleotide binding246 – 2538ATP Potential
Nucleotide binding372 – 3798ATP Potential
Region1 – 241241ManNAc-6-P epimerase HAMAP-Rule MF_01235
Region242 – 525284ManNAc kinase HAMAP-Rule MF_01235

Sequences

Sequence LengthMass (Da)Tools
Q8FWN5 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: D403CAE499AB9486

FASTA52553,867
        10         20         30         40         50         60 
MRGSPRNLCR VQGMIEEIVN RLRGKLIVSC QPVPESPFDN VASVVAYARA AEASGASGLR 

        70         80         90        100        110        120 
IEGAANVAAT AQASTLPVIG LIKRDLDDSP VRITPFLEDV AALCDAGAAI VAVDATDRRR 

       130        140        150        160        170        180 
PVPAAELIGE IKRRGRIAMA DISTLAEARN ALAAGADIIG TTMSGYTGEG PTPKDPDLDL 

       190        200        210        220        230        240 
VAHCSRLGSF LIAEGRYNSP QQAGEAIRAG ADAVVVGSAI TRPEHITGWF RDAVESAAKP 

       250        260        270        280        290        300 
SSPVLAFDIG GTKTLAALVR GREILERRVM TTPASVGSES WIGAIASLSA DWQGRYQRAA 

       310        320        330        340        350        360 
IAVTGRVDGE IWSSLNPETL AIPPDYPLGR RMGAALGAPV EVINDAQAAA WGENRFGAAR 

       370        380        390        400        410        420 
GRDMVFLTIS SGIGGGIVLG GRLIRGARGI AGSLGQVLVA GPSGFVRLET LASGFGIAKM 

       430        440        450        460        470        480 
ALEAGHAGDA RSVFSAAAAG EGWARRILLD AASQLAAAVA GLQAIVDPEC IVIGGGVGMA 

       490        500        510        520 
DGFLDMLREA LGSHSAVMRP DIVAAELGAD AGIIGVADLA ATYFS 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014292 Genomic DNA. Translation: AAN33608.1.
CP002998 Genomic DNA. Translation: AEM19887.1.
RefSeqNP_699603.1. NC_004311.2.
YP_005614113.1. NC_017250.1.

3D structure databases

ProteinModelPortalQ8FWN5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BRA0411.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN33608; AAN33608; BRA0411.
AEM19887; AEM19887; BS1330_II0408.
GeneID1164849.
12139843.
KEGGbms:BRA0411.
bsi:BS1330_II0408.
PATRIC17793616. VBIBruSui107850_2624.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1940.
HOGENOMHOG000289284.
KOK13967.
OMAREDIANC.
OrthoDBEOG6NSGKX.
ProtClustDBCLSK898967.

Enzyme and pathway databases

UniPathwayUPA00629; UER00681.
UPA00629; UER00682.

Family and domain databases

Gene3D3.20.20.70. 1 hit.
HAMAPMF_01235. ManNAc6P_epimer.
InterProIPR013785. Aldolase_TIM.
IPR007260. NanE.
IPR011060. RibuloseP-bd_barrel.
IPR000600. ROK.
[Graphical view]
PfamPF04131. NanE. 1 hit.
PF00480. ROK. 1 hit.
[Graphical view]
SUPFAMSSF51366. SSF51366. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNANEK_BRUSU
AccessionPrimary (citable) accession number: Q8FWN5
Secondary accession number(s): G0KCE9
Entry history
Integrated into UniProtKB/Swiss-Prot: March 28, 2003
Last sequence update: March 1, 2003
Last modified: April 16, 2014
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella suis

Brucella suis (strain 1330): entries and gene names