Q8FWG5 (BIOD_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
ATP-dependent dethiobiotin synthetase BioD
EC=6.3.3.3

Alternative name(s):
DTB synthetase
Short name=DTBS
Dethiobiotin synthase

Gene names

Name:	bioD
Ordered Locus Names:	BRA0490, BS1330_II0486

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	212 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes a mechanistically unusual reaction, the ATP-dependent insertion of CO2 between the N7 and N8 nitrogen atoms of 7,8-diaminopelargonic acid (DAPA) to form an ureido ring By similarity. HAMAP MF_00336
Catalytic activity	ATP + 7,8-diaminononanoate + CO2 = ADP + phosphate + dethiobiotin. HAMAP MF_00336
Cofactor	Magnesium By similarity. HAMAP MF_00336
Pathway	Cofactor biosynthesis; biotin biosynthesis; biotin from 7,8-diaminononanoate: step 1/2. HAMAP MF_00336
Subcellular location	Cytoplasm By similarity HAMAP MF_00336.
Sequence similarities	Belongs to the dethiobiotin synthetase family.

Ontologies

Keywords
Biological process	Biotin biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	biotin biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW dethiobiotin synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 212	212	ATP-dependent dethiobiotin synthetase BioD HAMAP MF_00336		PRO_0000187951
Regions
	Nucleotide binding	100 – 103	4	ATP By similarity
	Nucleotide binding	184 – 186	3	ATP By similarity
Sites
	Metal binding	17	1	Magnesium 2 By similarity
	Metal binding	100	1	Magnesium 2 By similarity
	Binding site	37	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FWG5 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F7D5083F8F33E1DC	FASTA	212	22,988
	10 20 30 40 50 60 MNSRLIVTGT DTGIGKTVFS AALCHALGAA YWKPVQSGLE EETDSEIVAR LAQASPQRIL 70 80 90 100 110 120 PEAWRLNTPA SPHLSARLDG VEIRPEEMHI PATSLPLVIE GAGGLLVPLN DKTLFADLFA 130 140 150 160 170 180 IWRIPAILCA RAALGTINHT LLSLEAMRSR DIPVLGVAFI GEANEDTETT IAHLGRVKRL 190 200 210 GRLPLLDDLS PEKLHHSFAR NFHIDDFAGV AR

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed: 22038969] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014292 Genomic DNA. Translation: AAN33682.1. CP002998 Genomic DNA. Translation: AEM19960.1.
RefSeq	NP_699677.1. NC_004311.2.
3D structure databases
ProteinModelPortal	Q8FWG5.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1164928.
GenomeReviews	Gene locus BRA0490 in contig AE014292_GR.
KEGG	bms:BRA0490.
NMPDR	fig|204722.1.peg.2586.
PATRIC	17793789. VBIBruSui107850_2710.
TIGR	BRA0490.
Phylogenomic databases
HOGENOM	HBG650065.
OMA	WKPIQSG.
PhylomeDB	Q8FWG5.
ProtClustDB	PRK00090.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_A0490-MONOMER.
Family and domain databases
HAMAP	MF_00336. BioD. [Tree]
InterPro	IPR004472. DTB_synth_BioD. [Graphical view]
KO	K01935.
PIRSF	PIRSF006755. DTB_synth. 1 hit.
TIGRFAMs	TIGR00347. BioD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

BIOD_BRUSU

Accession

Primary (citable) accession number: Q8FWG5
Secondary accession number(s): G0KCM2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program