Q8FVU9 (GCSP_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine dehydrogenase [decarboxylating]
EC=1.4.4.2

Alternative name(s):
Glycine cleavage system P-protein
Glycine decarboxylase

Gene names

Name:	gcvP
Ordered Locus Names:	BRA0725, BS1330_II0718

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	932 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity. HAMAP-Rule MF_00711
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP-Rule MF_00711
Cofactor	Pyridoxal phosphate By similarity. HAMAP-Rule MF_00711
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H By similarity.
Sequence similarities	Belongs to the GcvP family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP
Molecular_function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 932	932	Glycine dehydrogenase [decarboxylating] HAMAP-Rule MF_00711		PRO_0000166910
Amino acid modifications
	Modified residue	685	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FVU9 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: E3889FEE67C92252	FASTA	932	99,254
	10 20 30 40 50 60 MTEFLPFVAR HIGPRHEDER AMLAALGLPS METLITQAVP ASIRLNRALN LPAALSEADA 70 80 90 100 110 120 LAELGTIMGR NVVKKSFIGA GYHGVHTPPV IQRNLFENPA WYTAYTPYQS EISQGRLELL 130 140 150 160 170 180 FHFQTLVAEL TGLPVACASL LDEATAVAEA IGVACRHHRD KRSRILLAGE LHPQTVDVVN 190 200 210 220 230 240 TRAEPLGWEI ATGSDVDDNT AAIVVPWPDT RGVYGDFAKV IADAKAKGAL VIAVADPLAL 250 260 270 280 290 300 TIMEAPARWG ADMAVGSMQR YGVPMGFGGP HAAYLAVSEA LTRIIPGRIV GQSVDAHGRA 310 320 330 340 350 360 AYRLALQTRE QHIRRDKATS NICTAQALLA NMAAAFAIWH GPAGLQAIAT RVAALAARFA 370 380 390 400 410 420 AALKAAGVEI AGESLFDTVT AKVPGKAAAI AAEADKGGRL IRIIDADTVG VTFDETSTEE 430 440 450 460 470 480 DLTALASLFG AKPVGGDTVL VPGKERGEGF LTQEVFHSHR SETEMMRFLR RLADKDLALD 490 500 510 520 530 540 RAMIPLGSCT MKLNAAAEMM PVSWNTVANL HPFAPAEQVQ GYAKMTSDLE AWLCEITGFA 550 560 570 580 590 600 GVSLQPNAGS QGEYAGLMAI RHYHQAWGQG HRNICLIPSS AHGTNPASAS MAGMSVVVVN 610 620 630 640 650 660 CRPDGDIDID DLKAKAEKHR DNLAAFMITY PSTYGVFEEG IKAFCEIVHD NGGQVYFDGA 670 680 690 700 710 720 NLNALVGLAR PADIGADVCH MNLHKTFCIP HGGGGPGVGP IGVAKHLVPY LPGHVEAGSE 730 740 750 760 770 780 HAVAAAQFGS ASILVITWMY IRMMGGAGLK KATEAAILNA NYIAHRLKGV YPILYTGAHD 790 800 810 820 830 840 RVAHECIVDT RVLKDSAGIT VEDVAKRLID YGFHAPTMSW PVAGTLMIEP TESEPKLEID 850 860 870 880 890 900 RLCDAMIAIA GEAKKVADGV WPADDNPLAN APHTASDTLA TEWKHPYTRE EAVFPGGAFD 910 920 930 PTAKYWPPVS RVDNVGGDRN LICSCPPVAA YG

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014292 Genomic DNA. Translation: AAN33907.1. CP002998 Genomic DNA. Translation: AEM20183.1.
RefSeq	NP_699902.1. NC_004311.2. YP_005614409.1. NC_017250.1.
3D structure databases
ProteinModelPortal	Q8FVU9.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BRA0725.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN33907; AAN33907; BRA0725. AEM20183; AEM20183; BS1330_II0718.
GeneID	1165167. 12138946.
KEGG	bms:BRA0725. bsi:BS1330_II0718.
PATRIC	17794258. VBIBruSui107850_2940.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1003.
HOGENOM	HOG000239369.
KO	K00281.
OMA	PVSRIDN.
ProtClustDB	PRK05367.
Family and domain databases
Gene3D	3.40.640.10. 2 hits.
HAMAP	MF_00711. GcvP.
InterPro	IPR020580. GDC-P_N. IPR020581. GDC_P. IPR003437. GDC_P_homo. IPR015424. PyrdxlP-dep_Trfase. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
PANTHER	PTHR11773. PTHR11773. 1 hit.
Pfam	PF02347. GDC-P. 2 hits. [Graphical view]
SUPFAM	SSF53383. PyrdxlP-dep_Trfase_major. 2 hits.
TIGRFAMs	TIGR00461. gcvP. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GCSP_BRUSU

Accession

Primary (citable) accession number: Q8FVU9
Secondary accession number(s): G0KD95

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 70 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program