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Q8FVP6 (FOLD_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Bifunctional protein FolD

Including the following 2 domains:

  1. Methylenetetrahydrofolate dehydrogenase
    EC=1.5.1.5
  2. Methenyltetrahydrofolate cyclohydrolase
    EC=3.5.4.9
Gene names
Name:folD
Ordered Locus Names:BRA0781, BS1330_II0774
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the oxidation of 5,10-methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10-formyltetrahydrofolate By similarity. HAMAP-Rule MF_01576

Catalytic activity

5,10-methylenetetrahydrofolate + NADP+ = 5,10-methenyltetrahydrofolate + NADPH. HAMAP-Rule MF_01576

5,10-methenyltetrahydrofolate + H2O = 10-formyltetrahydrofolate. HAMAP-Rule MF_01576

Pathway

One-carbon metabolism; tetrahydrofolate interconversion. HAMAP-Rule MF_01576

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_01576

Sequence similarities

Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 299299Bifunctional protein FolD HAMAP-Rule MF_01576
PRO_0000268296

Regions

Nucleotide binding168 – 1703NADP By similarity

Sites

Binding site1931NADP By similarity
Binding site2341NADP; via amide nitrogen By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FVP6 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 7446B7B964FB967A

FASTA29931,231
        10         20         30         40         50         60 
MAQLIDGKKL AEDVVSTVKT ETEKLVAATG VVPGIAVVIV GEDPASQVYV ASKSRKAKEC 

        70         80         90        100        110        120 
GFHSVQHDLP ETASEQELLN LIEGLNNDPA IHGILVQLPL PGHIDSGRVI QTIAPEKDVD 

       130        140        150        160        170        180 
GFHFINVGKL GTGEVETAFV PCTPAGAMIM IERVHGRDLS GLNAVVIGRS NIVGKPMFNL 

       190        200        210        220        230        240 
LLAANATVTV AHSRTKDLPA IARNADILVA AVGRPQMVKG DWVKPGATVI DVGINRIPAP 

       250        260        270        280        290 
ERGEGKTRLV GDVDFAEAEK VAGAITPVPG GVGPMTIAML MANTLTAACR SAGMKKPVF 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014292 Genomic DNA. Translation: AAN33961.1.
CP002998 Genomic DNA. Translation: AEM20237.1.
RefSeqNP_699956.1. NC_004311.2.
YP_005614463.1. NC_017250.1.

3D structure databases

ProteinModelPortalQ8FVP6.
SMRQ8FVP6. Positions 2-292.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BRA0781.

Proteomic databases

PRIDEQ8FVP6.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN33961; AAN33961; BRA0781.
AEM20237; AEM20237; BS1330_II0774.
GeneID1165223.
12139002.
KEGGbms:BRA0781.
bsi:BS1330_II0774.
PATRIC17794369. VBIBruSui107850_2995.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0190.
HOGENOMHOG000218242.
KOK01491.
OMADLKHKIQ.
OrthoDBEOG6K6VBB.
ProtClustDBPRK14188.

Enzyme and pathway databases

UniPathwayUPA00193.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_01576. THF_DHG_CYH.
InterProIPR016040. NAD(P)-bd_dom.
IPR000672. THF_DH/CycHdrlase.
IPR020630. THF_DH/CycHdrlase_cat_dom.
IPR020867. THF_DH/CycHdrlase_CS.
IPR020631. THF_DH/CycHdrlase_NAD-bd_dom.
[Graphical view]
PfamPF00763. THF_DHG_CYH. 1 hit.
PF02882. THF_DHG_CYH_C. 1 hit.
[Graphical view]
PRINTSPR00085. THFDHDRGNASE.
PROSITEPS00766. THF_DHG_CYH_1. 1 hit.
PS00767. THF_DHG_CYH_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameFOLD_BRUSU
AccessionPrimary (citable) accession number: Q8FVP6
Secondary accession number(s): G0KDE9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 12, 2006
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella suis

Brucella suis (strain 1330): entries and gene names