Reviewed, UniProtKB/Swiss-Prot Q8FVF3 (LEU3_BRUSU)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	BRA0890

Organism

Brucella suis [Complete proteome] [HAMAP]

Taxonomic identifier

29461 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella

Protein attributes

Sequence length	370 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate. HAMAP MF_01033
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD+ = 4-methyl-2-oxopentanoate + CO2 + NADH. HAMAP MF_01033
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity. HAMAP MF_01033
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4. HAMAP MF_01033
Subunit structure	Homodimer By similarity. HAMAP MF_01033
Subcellular location	Cytoplasm By similarity HAMAP MF_01033.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP NAD or NADH binding Inferred from electronic annotation. Source: InterPro magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 370	370	3-isopropylmalate dehydrogenase HAMAP MF_01033		PRO_0000083654
Regions
	Nucleotide binding	77 – 90	14	NAD By similarity
	Nucleotide binding	290 – 302	13	NAD By similarity
Sites
	Metal binding	226	1	Magnesium or manganese By similarity
	Metal binding	250	1	Magnesium or manganese By similarity
	Metal binding	254	1	Magnesium or manganese By similarity
	Binding site	97	1	Substrate By similarity
	Binding site	107	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	226	1	Substrate By similarity
	Site	142	1	Important for catalysis By similarity
	Site	193	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FVF3-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F9E81A0B300537CB	FASTA	370	39,756
	10 20 30 40 50 60 MASRKLLLLP GDGIGPEAMA EVRKVIAFLN SDLNLGFETE EGLVGGCAYD AHGQAISDAD 70 80 90 100 110 120 MEKALAADAV LFGAVGGPKW DSVPYEVRPE AGLLRLRKDM QLYANLRPAI CYPALAHSSS 130 140 150 160 170 180 LKPEVIEGLD ILILRELTGG VYFGEPKEII DLGNGQKRGI DTQVYDTYEI ERIADVAFEL 190 200 210 220 230 240 ARTRRNKVTS MEKRNVMKSG VLWNQVVTAR HKEKHADVQL EHMLADAGGM QLVRWPKQFD 250 260 270 280 290 300 VILTDNLFGD LLSDVAAMLT GSLGMLPSAS LGAADSKTGK RKALYEPVHG SAPDIAGKGI 310 320 330 340 350 360 ANPIAMIASL AMCLRYSFGL VAEADRLEAA IAGVLDDGIR TADIWSEGNT KVGTTEMGDA 370 ILAKFKALSA

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed: 12271122] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330 / Biovar 1.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014292 Genomic DNA. Translation: AAN34062.1.
RefSeq	NP_700057.1.
3D structure databases
SMR	Q8FVF3. Positions 4-365.
ModBase	Search...
Genome annotation databases
GeneID	1165333.
GenomeReviews	Gene locus BRA0890 in contig AE014292_GR.
KEGG	bms:BRA0890.
NMPDR	fig|204722.1.peg.2966.
TIGR	BRA0890.
Phylogenomic databases
HOGENOM	HBG518924.
OMA	MSYQIAV.
PhylomeDB	Q8FVF3.
Enzyme and pathway databases
BioCyc	BSUI204722:BR_A0890-MONOMER.
BRENDA	1.1.1.85. 281610.
Family and domain databases
HAMAP	MF_01033. LeuB_type1. [Tree]
InterPro	IPR019818. IsoCit/isopropylmalate_DH_CS. IPR001804. Isocitrate/isopropylmalate_DH. IPR004429. Isopropylmalate_DH. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_BRUSU

Accession

Primary (citable) accession number: Q8FVF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 7, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 49 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)