Q8FV30 (RNPA_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease P protein component
Short name=RNase P protein
Short name=RNaseP protein
EC=3.1.26.5

Alternative name(s):
Protein C5

Gene names

Name:	rnpA
Ordered Locus Names:	BRA1022, BS1330_II1014

Organism

Brucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]

Taxonomic identifier

204722 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Alphaproteobacteria › Rhizobiales › Brucellaceae › Brucella › Brucella suis

Protein attributes

Sequence length	141 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	RNaseP catalyzes the removal of the 5'-leader sequence from pre-tRNA to produce the mature 5'-terminus. It can also cleave other RNA substrates such as 4.5S RNA. The protein component plays an auxiliary but essential role in vivo by binding to the 5'-leader sequence and broadening the substrate specificity of the ribozyme By similarity. HAMAP-Rule MF_00227
Catalytic activity	Endonucleolytic cleavage of RNA, removing 5'-extranucleotides from tRNA precursor. HAMAP-Rule MF_00227
Subunit structure	Consists of a catalytic RNA component (M1 or RnpB) and a protein subunit By similarity.
Sequence similarities	Belongs to the RnpA family.
Sequence caution	The sequence AAN34190.1 differs from that shown. Reason: Erroneous initiation. The sequence AEM20466.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	RNA-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC tRNA 5'-leader removal Inferred from electronic annotation. Source: HAMAP
Molecular_function	ribonuclease P activity Inferred from electronic annotation. Source: HAMAP tRNA binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 141	141	Ribonuclease P protein component HAMAP-Rule MF_00227		PRO_0000198434

Sequences

	Sequence		Length	Mass (Da)
	Q8FV30 [UniParc]. Last modified March 28, 2003. Version 2. Checksum: F7A2FA43596B5EE1	FASTA	141	16,252
	10 20 30 40 50 60 MKSKKQILRL RKRAEFLTVR NGEKRRGPLF LMEVRERTEE ESNAAKTGDN PRVGFTVTKK 70 80 90 100 110 120 NGNAVIRNRI RRRLKEAIRC HAGRDMAPST DYVIVAREQA LNAPFSQLTE ELSRRITAKG 130 140 ERRSGRKRRT ERPEPGPVNG K

References

[1]

"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts." Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M. Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

[2]

"Revised genome sequence of Brucella suis 1330." Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R. J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 1330.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AE014292 Genomic DNA. Translation: AAN34190.1. Different initiation. CP002998 Genomic DNA. Translation: AEM20466.1. Different initiation.
RefSeq	NP_700185.1. NC_004311.2. YP_005614692.1. NC_017250.1.
3D structure databases
ProteinModelPortal	Q8FV30.
ModBase	Search...
Protein-protein interaction databases
STRING	204722.BRA1022.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAN34190; AAN34190; BRA1022. AEM20466; AEM20466; BS1330_II1014.
GeneID	1165467. 12139246.
KEGG	bms:BRA1022. bsi:BS1330_II1014.
PATRIC	17794879. VBIBruSui107850_3247.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0594.
HOGENOM	HOG000266302.
KO	K03536.
ProtClustDB	PRK01313.
Family and domain databases
Gene3D	3.30.230.10. 1 hit.
HAMAP	MF_00227. RNase_P.
InterPro	IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. IPR000100. RNase_P. IPR020539. RNase_P_CS. [Graphical view]
Pfam	PF00825. Ribonuclease_P. 1 hit. [Graphical view]
ProDom	PD003629. Ribonuclease_P. 1 hit. [Graphical view] [Entries sharing at least one domain]
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00188. rnpA. 1 hit.
PROSITE	PS00648. RIBONUCLEASE_P. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

RNPA_BRUSU

Accession

Primary (citable) accession number: Q8FV30
Secondary accession number(s): G0KE28

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 28, 2003
Last sequence update:	March 28, 2003
Last modified:	May 1, 2013
This is version 61 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program