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Q8FV07 (DAPB_BRUSU) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 78. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
4-hydroxy-tetrahydrodipicolinate reductase

Short name=HTPA reductase
EC=1.17.1.8
Gene names
Name:dapB
Ordered Locus Names:BRA1051, BS1330_II1043
OrganismBrucella suis biovar 1 (strain 1330) [Complete proteome] [HAMAP]
Taxonomic identifier204722 [NCBI]
Taxonomic lineageBacteriaProteobacteriaAlphaproteobacteriaRhizobialesBrucellaceaeBrucella

Protein attributes

Sequence length268 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the conversion of 4-hydroxy-tetrahydrodipicolinate (HTPA) to tetrahydrodipicolinate By similarity. HAMAP-Rule MF_00102

Catalytic activity

(S)-2,3,4,5-tetrahydropyridine-2,6-dicarboxylate + NAD(P)+ + H2O = (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinate + NAD(P)H. HAMAP-Rule MF_00102

Pathway

Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 4/4. HAMAP-Rule MF_00102

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00102.

Sequence similarities

Belongs to the DapB family.

Caution

Was originally thought to be a dihydrodipicolinate reductase (DHDPR), catalyzing the conversion of dihydrodipicolinate to tetrahydrodipicolinate. However, it was shown in E.coli that the substrate of the enzymatic reaction is not dihydrodipicolinate (DHDP) but in fact (2S,4S)-4-hydroxy-2,3,4,5-tetrahydrodipicolinic acid (HTPA), the product released by the DapA-catalyzed reaction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 2682684-hydroxy-tetrahydrodipicolinate reductase HAMAP-Rule MF_00102
PRO_0000141418

Regions

Nucleotide binding7 – 126NAD(P) By similarity
Nucleotide binding97 – 993NAD(P) By similarity
Nucleotide binding121 – 1244NAD(P) By similarity
Region165 – 1662Substrate binding By similarity

Sites

Active site1551Proton donor/acceptor By similarity
Active site1591Proton donor By similarity
Binding site331NAD By similarity
Binding site341NADP By similarity
Binding site1561Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FV07 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 0B31C8F454C0B25D

FASTA26827,593
        10         20         30         40         50         60 
MGLVVVGAGG RMGQTLIRTI QSIEGAKLVG AIERSGSPFL GKDAGEVTGI GTLGVAITDD 

        70         80         90        100        110        120 
PLPVFAKAHG VLDFTSPAAS VEFAGLAAQA RIVHVTGTTG CSAEDDEKIR AAARHATIVK 

       130        140        150        160        170        180 
SGNMSLGVNL LSVLVQKAAE ALGPEDFDIE ILEMHHRHKV DAPSGTALLL GEAAARGRDI 

       190        200        210        220        230        240 
ALADNSVRVR DGYTGPRETG AIGFATLRGG SVIGDHSVIL AGTGERVVLS HHAEDRSIFA 

       250        260 
RGAIKAALWA HGKKPGLYSM LDVLGLNT 

« Hide

References

[1]"The Brucella suis genome reveals fundamental similarities between animal and plant pathogens and symbionts."
Paulsen I.T., Seshadri R., Nelson K.E., Eisen J.A., Heidelberg J.F., Read T.D., Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C., DeBoy R.T., Durkin A.S., Kolonay J.F., Madupu R., Nelson W.C., Ayodeji B., Kraul M. expand/collapse author list , Shetty J., Malek J.A., Van Aken S.E., Riedmuller S., Tettelin H., Gill S.R., White O., Salzberg S.L., Hoover D.L., Lindler L.E., Halling S.M., Boyle S.M., Fraser C.M.
Proc. Natl. Acad. Sci. U.S.A. 99:13148-13153(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.
[2]"Revised genome sequence of Brucella suis 1330."
Tae H., Shallom S., Settlage R., Preston D., Adams L.G., Garner H.R.
J. Bacteriol. 193:6410-6410(2011) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 1330.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
AE014292 Genomic DNA. Translation: AAN34218.1.
CP002998 Genomic DNA. Translation: AEM20495.1.
RefSeqNP_700213.1. NC_004311.2.
YP_005614721.1. NC_017250.1.

3D structure databases

ProteinModelPortalQ8FV07.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING204722.BRA1051.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAN34218; AAN34218; BRA1051.
AEM20495; AEM20495; BS1330_II1043.
GeneID1165496.
12139276.
KEGGbms:BRA1051.
bsi:BS1330_II1043.
PATRIC17794937. VBIBruSui107850_3276.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0289.
HOGENOMHOG000227153.
KOK00215.
OMAIVMAPNM.
OrthoDBEOG6SV5DS.
ProtClustDBPRK00048.

Enzyme and pathway databases

UniPathwayUPA00034; UER00018.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
HAMAPMF_00102. DapB.
InterProIPR022663. DapB_C.
IPR000846. DapB_N.
IPR022664. DapB_N_CS.
IPR011770. Dihydrodipicolinate_Rdtase.
IPR023940. Dihydrodipicolinate_Rdtase_bac.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR20836. PTHR20836. 1 hit.
PfamPF05173. DapB_C. 1 hit.
PF01113. DapB_N. 1 hit.
[Graphical view]
PIRSFPIRSF000161. DHPR. 1 hit.
TIGRFAMsTIGR00036. dapB. 1 hit.
PROSITEPS01298. DAPB. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDAPB_BRUSU
AccessionPrimary (citable) accession number: Q8FV07
Secondary accession number(s): G0KE57
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2003
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Brucella suis

Brucella suis (strain 1330): entries and gene names