Reviewed, UniProtKB/Swiss-Prot Q8FTN4 (ARGJ_COREF)

Names and origin

Protein names

Recommended name:
    Glutamate N-acetyltransferase
    EC=2.3.1.35
Alternative name(s):
    Ornithine acetyltransferase
    Ornithine transacetylase
      Short name=OATase
Cleaved into the following 2 chains:
    1- Recommended name:
            Glutamate N-acetyltransferase alpha chain
    2- Recommended name:
            Glutamate N-acetyltransferase beta chain

Gene names

Name:	argJ
Ordered Locus Names:	CE1527

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	N(2)-acetyl-L-ornithine + L-glutamate = L-ornithine + N-acetyl-L-glutamate. HAMAP MF_01106
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; L-ornithine and N-acetyl-L-glutamate from L-glutamate and N(2)-acetyl-L-ornithine (cyclic): step 1/1. HAMAP MF_01106
Subunit structure	Heterotetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	Some bacteria possess a monofunctional argJ, i.e., capable of catalyzing only the fifth step of the arginine biosynthetic pathway. HAMAP MF_01106
Sequence similarities	Belongs to the argJ family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis
Cellular component	Cytoplasm
Molecular function	Acyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	amino-acid N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP glutamate N-acetyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 182	182	Glutamate N-acetyltransferase alpha chain By similarity		PRO_0000002159
	Chain	183 – 388	206	Glutamate N-acetyltransferase beta chain By similarity		PRO_0000002160
Sites
	Site	182 – 183	2	Cleavage; by autolysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FTN4-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 3C298BFD2466730E	FASTA	388	39,543
	10 20 30 40 50 60 MAQTGITAPK GFVASATTAG IKPSGKPDMA LVVNQGPEYT AAAVFTRNRV IASPVKVSRE 70 80 90 100 110 120 NVADGQLKAV LYNAGNANAC NGTQGDADAR ESVNTLATKL GLAPEDIGVC STGLIGELLP 130 140 150 160 170 180 MDKVTAGIEA LTAEGALGDN GAAAAQAIMT TDTVDKETVV FADGWTVGAM GKGVGMMAPS 190 200 210 220 230 240 LATMLVCLTT DASITPAMAR IALAKATAVT FETLDIDGST STNDTVFLLA SGASGVKPTQ 250 260 270 280 290 300 DELNDAVFAA CSDLAEQMQA DAEGVTKRVS VTVTGTTTDE MAINAARTIA RDNLFKCAMF 310 320 330 340 350 360 GSDPNWGRVL AAVGMADADM DPDHIAVYFN DQAVCLDSTG APGAREVDLS GADIAVRVDL 370 380 GAGGEGQATV RTTDLSYSYV EINSAYST

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000035 Genomic DNA. Translation: BAC18337.1.
RefSeq	NP_738137.1.
3D structure databases
ModBase	Search...
Protein family/group databases
MEROPS	T05.001.
Genome annotation databases
GeneID	1031984.
GenomeReviews	Gene locus CE1527 in contig BA000035_GR.
KEGG	cef:CE1527.
NMPDR	fig|196164.1.peg.1527.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8FTN4.
OMA	VHENSAY.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1527-MON.
BRENDA	2.3.1.35. 277326.
Family and domain databases
HAMAP	MF_01106. [Tree]
InterPro	IPR002813. Arg_biosynth_ArgJ. [Graphical view]
PANTHER	PTHR23100. ArgJ. 1 hit.
Pfam	PF01960. ArgJ. 1 hit. [Graphical view]
ProDom	PD004193. ArgJ. 2 hits. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00120. ArgJ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ARGJ_COREF

Accession

Primary (citable) accession number: Q8FTN4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)