Reviewed, UniProtKB/Swiss-Prot Q8FTD0 (SYC2_COREF)

Names and origin

Protein names

Recommended name:
    Putative cysteinyl-tRNA synthetase 2
    EC=6.1.1.16
Alternative name(s):
    Cysteine--tRNA ligase 2
      Short name=CysRS 2

Gene names

Name:	cysS2
Ordered Locus Names:	CE1639

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	430 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00041
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP cysteine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 430	430	Putative cysteinyl-tRNA synthetase 2 HAMAP MF_00041		PRO_0000159387
Regions
	Motif	50 – 60	11	"HIGH" region HAMAP MF_00041
	Motif	294 – 298	5	"KMSKS" region HAMAP MF_00041
Sites
	Binding site	297	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FTD0-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 1494EABC26B507D2	FASTA	430	46,764
	10 20 30 40 50 60 MHSWPTPDIP ALDGTPVPLA LYDTADQQVK PVEVPPSGSG TPVGMYVCGI TPYDSTHLGH 70 80 90 100 110 120 AATYLAFDLI YRVLLDNDHQ VHYVQNITDV DDPLFERAER DGVDWRELGT SQIDLFRKDM 130 140 150 160 170 180 ETLAVIPPKD YIGAIESIDE VIEMVSTLLE EGAAYVVDDP EYPDVYASIN ATENFGYESN 190 200 210 220 230 240 YDAATMAELF AERGGDPDRP GKRNPMDALL WRAAREGEPS WESPFGPGRP GWHIECSAIA 250 260 270 280 290 300 TNRLGHSFDI QGGGSDLMFP HHEFSAAHAE AAHGVERMAK HYVHAGMISQ DGVKMSKSLG 310 320 330 340 350 360 NLEFVHRLTA AGHEPGAIRL GVYAHHYRGD RDWSDEILAA AEARLALWRS AITVATDVQA 370 380 390 400 410 420 AVDAVAQLRT HLSNDLDTPA ALAAVDAWAE AALKDTGNVS DTADQFDTPE FTPAGRILAA 430 AVDALLGVRL

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000035 Genomic DNA. Translation: BAC18449.1.
RefSeq	NP_738249.1.
3D structure databases
HSSP	HSSP built from PDB template 1LI5 based on UniProtKB P21888.
ModBase	Search...
Genome annotation databases
GeneID	1032008.
GenomeReviews	Gene locus CE1639 in contig BA000035_GR.
KEGG	cef:CE1639.
NMPDR	fig|196164.1.peg.1639.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8FTD0.
OMA	HYVQNVT.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1639-MON.
BRENDA	6.1.1.16. 277326.
Family and domain databases
HAMAP	MF_00041. Divergent sequence. [Tree]
InterPro	IPR001412. aa-tRNA-synth_I_CS. IPR015804. Cys-tRNA-synt_Ia_C. IPR015803. Cys-tRNA-synt_Ia_N. IPR017812. Mycothiol_biosynthesis_MshC. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
TIGRFAMs	TIGR03447. mycothiol_MshC. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC2_COREF

Accession

Primary (citable) accession number: Q8FTD0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 15, 2005
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)