Reviewed, UniProtKB/Swiss-Prot Q8FTA8 (ACON_COREF)

Names and origin

Protein names

Recommended name:
    Aconitate hydratase
    EC=4.2.1.3

Gene names

Name:	acn
Ordered Locus Names:	CE1661

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	937 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	Citrate = isocitrate.
Cofactor	Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle.
Induction	Repressed by acnR By similarity.
Sequence similarities	Belongs to the aconitase/IPM isomerase family.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Ligand	Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: InterPro aconitate hydratase activity Inferred from electronic annotation. Source: EC iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 937	937	Aconitate hydratase		PRO_0000076659
Sites
	Metal binding	475	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	541	1	Iron-sulfur (4Fe-4S) By similarity
	Metal binding	544	1	Iron-sulfur (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FTA8-1 [UniParc]. Last modified February 7, 2006. Version 2. Checksum: D23E4FD27C675273	FASTA	937	102,106
	10 20 30 40 50 60 MTESKNSFNA KSTLQVGEKS YDYYALSAVP GMEKLPYSLK VLGENLLRTE DGANITEEHI 70 80 90 100 110 120 EAIANWDASA DPSIEIQFTP ARVLMQDFTG VPCVVDLATM REAVKTLGGD PDKVNPLNPA 130 140 150 160 170 180 EMVIDHSVIV EAFGRPDALE KNVEIEYERN EERYQFLRWG SEAFSNFRVV PPGTGIVHQV 190 200 210 220 230 240 NIEYLARVVF DNDGLAYPDT CIGTDSHTTM ENGLGILGWG VGGIEAEAAM LGQPVSMLIP 250 260 270 280 290 300 RVVGFKLTGE IPVGVTATDV VLTITEMLRD HGVVQKFVEF YGNGVKSVPL ANRATIGNMS 310 320 330 340 350 360 PEFGSTCAMF PIDEETIKYL RLTGRPEEQI ALVEAYAKAQ GMWLEQDAPE AEYSEYLELD 370 380 390 400 410 420 LSTVVPSIAG PKRPQDRILL SEAKEQFRED LKAYTNDPVQ VDQSIPAKRM ANEGGFQPGS 430 440 450 460 470 480 TSDLDNYNAS WPGEGESAAA NAEGRPSNPV TVVSPQGGEY TIDHGMVAIA SITSCTNTSN 490 500 510 520 530 540 PSVMIGAGLI ARKAAEKGLK SKPWVKTICA PGSQVVDGYY QRADLWKDLE ALGFYLSGFG 550 560 570 580 590 600 CTTCIGNSGP LPEEISEAIN EHDLAATAVL SGNRNFEGRI SPDVKMNYLA SPIMVIAYAI 610 620 630 640 650 660 AGTMDFDFEN EALGQDQDGN DVFLKDIWPS TEEIEETIQA AISRELYEAD YADVFKGDKQ 670 680 690 700 710 720 WQELDIPSGK TFEWDENSTY IRKAPYFDGM TAEPQPVTDI ENARVLAKLG DSVTTDHISP 730 740 750 760 770 780 ASSIKPGTPA AQYLDAHGVE RQDYNSLGSR RGNHEVMMRG TFANIRLQNQ LVDIAGGYTR 790 800 810 820 830 840 DFTQEGGPQA FIYDACVNYK EAGIPLVVLA GKEYGTGSSR DWAAKGTNLL GVRAVITESF 850 860 870 880 890 900 ERIHRSNLIG MGVVPLQFPE GESHESLGLD GTETFDITGL TALNEGTTPK TVKVTATKEN 910 920 930 GEKVEFDAVV RIDTPGEADY FRHGGILQYV LRQMAAS

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000035 Genomic DNA. Translation: BAC18471.1. Different initiation.
RefSeq	NP_738271.1.
3D structure databases
HSSP	HSSP built from PDB template 7ACN based on UniProtKB P16276.
ModBase	Search...
Genome annotation databases
GeneID	1032014.
GenomeReviews	Gene locus CE1661 in contig BA000035_GR.
KEGG	cef:CE1661.
NMPDR	fig|196164.1.peg.1661.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8FTA8.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1661-MON.
BRENDA	4.2.1.3. 277326.
Family and domain databases
InterPro	IPR015931. Acnase/IPM_dHydase_lsu_aba_1/3. IPR001030. Acoase/IPM_deHydtase_lsu_aba. IPR015937. Aconitase-like_core. IPR015928. Aconitase/3IPM_dehydase_swvl. IPR006249. Aconitase/Fe_reg_prot_2. IPR015934. Aconitase/Fe_reg_prot_2/AcnD. IPR015932. Aconitase/IPMdHydase_lsu_aba_2. IPR018136. Aconitase_4Fe-4S_BS. IPR000573. AconitaseA/IPMdHydase_ssu_swvl. [Graphical view]
Gene3D	G3DSA:3.30.499.10. Acnase/IPM_dHydase_lsu_aba_1/3. 2 hits. G3DSA:3.20.19.10. Aconitase/3IPM_dehydase_swvl. 1 hit. G3DSA:3.40.1060.10. Aconitase/IPMdHydase_lsu_aba_2. 1 hit.
PANTHER	PTHR11670. Aconitase-like_core. 1 hit. PTHR11670:SF1. Aconitase/Fe_reg_prot_2/AcnD. 1 hit.
Pfam	PF00330. Aconitase. 1 hit. PF00694. Aconitase_C. 1 hit. [Graphical view]
PRINTS	PR00415. ACONITASE.
ProDom	PD000511. Aconitase_N. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR01341. aconitase_1. 1 hit.
PROSITE	PS00450. ACONITASE_1. 1 hit. PS01244. ACONITASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ACON_COREF

Accession

Primary (citable) accession number: Q8FTA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 7, 2006
Last sequence update:	February 7, 2006
Last modified:	March 3, 2009
This is version 43 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)