Reviewed, UniProtKB/Swiss-Prot Q8FT51 (DEF1_COREF)

Names and origin

Protein names

Recommended name:
    Peptide deformylase 1
      Short name=PDF 1
    EC=3.5.1.88
Alternative name(s):
    Polypeptide deformylase 1

Gene names

Name:	def1
Ordered Locus Names:	CE1720

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions By similarity.
Catalytic activity	Formyl-L-methionyl peptide + H2O = formate + methionyl peptide. HAMAP MF_00163
Cofactor	Binds 1 Fe2+ ion By similarity.
Sequence similarities	Belongs to the polypeptide deformylase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Ligand	Iron Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: HAMAP
Molecular function	iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW peptide deformylase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Peptide deformylase 1 HAMAP MF_00163		PRO_0000082772
Sites
	Active site	136	1	By similarity
	Metal binding	93	1	Iron By similarity
	Metal binding	135	1	Iron By similarity
	Metal binding	139	1	Iron By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FT51-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 1C6EC9BDFDE25B05	FASTA	169	18,445
	10 20 30 40 50 60 MTVRDVRIFG DPVLTSRADE VVDFDESLAT LIDDMFDTME DAGGVGLAAN QVGVLRRVFV 70 80 90 100 110 120 FDCSHVDGGL RGHVVNPVWE PIGEETQTGK EGCLSIPDVS AETTRYETVK LSGQDRDGNP 130 140 150 160 IGLVASGLLS RCIQHETDHL DGVLFLKRLD PAERKAAMGV IRASDWFNK

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000035 Genomic DNA. Translation: BAC18530.1.
RefSeq	NP_738330.1.
3D structure databases
HSSP	HSSP built from PDB template 2DEF based on UniProtKB P27251.
ModBase	Search...
Genome annotation databases
GeneID	1034213.
GenomeReviews	Gene locus CE1720 in contig BA000035_GR.
KEGG	cef:CE1720.
NMPDR	fig|196164.1.peg.1720.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8FT51.
OMA	Q8FT51. MAIRRIC.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1720-MON.
BRENDA	3.5.1.88. 277326.
Family and domain databases
HAMAP	MF_00163. [Tree]
InterPro	IPR000181. Fmet_deformylase. [Graphical view]
Gene3D	G3DSA:3.90.45.10. Fmet_deformylase. 1 hit.
PANTHER	PTHR10458. Fmet_deformylase. 1 hit.
Pfam	PF01327. Pep_deformylase. 1 hit. [Graphical view]
PIRSF	PIRSF004749. Pep_def. 1 hit.
PRINTS	PR01576. PDEFORMYLASE.
ProDom	PD003844. Fmet_deformylase. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00079. pept_deformyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DEF1_COREF

Accession

Primary (citable) accession number: Q8FT51

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2003
Last modified:	June 16, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)