Reviewed, UniProtKB/Swiss-Prot Q8FT41 (CARA_COREF)

Names and origin

Protein names

Recommended name:
    Carbamoyl-phosphate synthase small chain
    EC=6.3.5.5
Alternative name(s):
    Carbamoyl-phosphate synthetase glutamine chain

Gene names

Name:	carA
Ordered Locus Names:	CE1730

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	400 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2 ATP + L-glutamine + HCO3- + H2O = 2 ADP + phosphate + L-glutamate + carbamoyl phosphate. HAMAP MF_01209
Pathway	Amino-acid biosynthesis; L-arginine biosynthesis; carbamoyl phosphate from bicarbonate: step 1/1. HAMAP MF_01209 Pyrimidine metabolism; UMP biosynthesis via de novo pathway; (S)-dihydroorotate from bicarbonate: step 1/3. HAMAP MF_01209
Subunit structure	Composed of two chains; the small (or glutamine) chain promotes the hydrolysis of glutamine to ammonia, which is used by the large (or ammonia) chain to synthesize carbamoyl phosphate By similarity. HAMAP MF_01209
Sequence similarities	Belongs to the carA family. Contains 1 glutamine amidotransferase type-1 domain.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Arginine biosynthesis Pyrimidine biosynthesis
Domain	Glutamine amidotransferase
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	arginine biosynthetic process Inferred from electronic annotation. Source: HAMAP glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW pyrimidine nucleotide biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 400	400	Carbamoyl-phosphate synthase small chain HAMAP MF_01209		PRO_0000112270
Regions
	Domain	200 – 395	196	Glutamine amidotransferase type-1
	Region	1 – 199	199	CPSase HAMAP MF_01209
Sites
	Active site	278	1	Nucleophile By similarity
	Active site	368	1	By similarity
	Active site	370	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FT41-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: CFEE077BA9504756	FASTA	400	42,885
	10 20 30 40 50 60 MSNETNANST DSRQGVTNIG SVPAYLVLAD GRTFKGFGFG AIGTTLGEAV FTTAMTGYQE 70 80 90 100 110 120 TMTDPSYHRQ IVVATAPQIG NTGWNEEDNE SHDGSIWVAG LVIRDLAVRV SNWRATTTLQ 130 140 150 160 170 180 EEMAKQGVVG IGGIDTRALV RHIRNEGAVP AGIFSGADAE RPIEELVEIV KSQPSMVGAN 190 200 210 220 230 240 LAVEVSVDKP YVIEAEGEAR HTVVAYDLGI KQNTPRRFAA RGVRTVIVPA ETPFEEIKQY 250 260 270 280 290 300 NPSGVFISNG PGDPAAADIM VNIVREVLAA DIPFFGICFG NQILGRAFGM ETYKLKFGHR 310 320 330 340 350 360 GINVPVKNHI TGKIDITAQN HGFALKGEAG QEFETDFGTA VVTHTCLNDG VVEGVALKSG 370 380 390 400 RAYSVQYHPE AAAGPNDASP LFDQFVALMD EDSENQKEEA

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC18540.1.
RefSeq	NP_738340.1.
3D structure databases
SMR	Q8FT41. Positions 23-394.
ModBase	Search...
Genome annotation databases
GeneID	1034230.
GenomeReviews	Gene locus CE1730 in contig BA000035_GR.
KEGG	cef:CE1730.
NMPDR	fig|196164.1.peg.1730.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG286341.
OMA	FTYPELG.
PhylomeDB	Q8FT41.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1730-MONOMER.
BRENDA	6.3.5.5. 277326.
Family and domain databases
HAMAP	MF_01209_B. CPSase_S_chain_B. [Tree]
InterPro	IPR006220. Anth_synthII. IPR001317. CarbamoylP_synth_GATase_dom. IPR006274. CarbamoylP_synth_ssu. IPR002474. CarbamoylP_synth_ssu_N. IPR011702. GATASE. IPR017926. GATASE_1. IPR000991. GATase_class1_C. [Graphical view]
PANTHER	PTHR11405:SF4. CarA_synth_small. 1 hit.
Pfam	PF00988. CPSase_sm_chain. 1 hit. PF00117. GATase. 1 hit. [Graphical view]
PRINTS	PR00097. ANTSNTHASEII. PR00099. CPSGATASE. PR00096. GATASE.
TIGRFAMs	TIGR01368. CPSaseIIsmall. 1 hit.
PROSITE	PS51273. GATASE_TYPE_1. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CARA_COREF

Accession

Primary (citable) accession number: Q8FT41

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 55 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)