Reviewed, UniProtKB/Swiss-Prot Q8FT30 (AROK_COREF)

Names and origin

Protein names

Recommended name:
    Shikimate kinase
      Short name=SK
    EC=2.7.1.71

Gene names

Name:	aroK
Ordered Locus Names:	CE1741

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	169 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate By similarity.
Catalytic activity	ATP + shikimate = ADP + shikimate 3-phosphate. HAMAP MF_00109
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 5/7. HAMAP MF_00109
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Probable.
Sequence similarities	Belongs to the shikimate kinase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP shikimate kinase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 169	169	Shikimate kinase HAMAP MF_00109		PRO_0000192375
Regions
	Nucleotide binding	13 – 18	6	ATP By similarity
Sites
	Metal binding	17	1	Magnesium By similarity
	Binding site	35	1	Substrate By similarity
	Binding site	59	1	Substrate By similarity
	Binding site	80	1	Substrate; via amide nitrogen By similarity
	Binding site	117	1	ATP By similarity
	Binding site	136	1	Substrate By similarity
	Binding site	153	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FT30-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 0B72827B1FC0508E	FASTA	169	18,543
	10 20 30 40 50 60 MARPIVVLVG PPGAGKSTIG RRLARALNAD LVDSDELIEK ATGKACGEVF SELGEPAFRE 70 80 90 100 110 120 LEAHHVAEAL NHDGVVSLGG GAILTESTRE LLREHDVVWI DVSVAEGVRR TAGERTRPVL 130 140 150 160 AADDPVEHYR NLLETRRPLY EEVSTFRVRT NSRSPQQVVA EILHHLEDD

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000035 Genomic DNA. Translation: BAC18551.1.
RefSeq	NP_738351.1.
3D structure databases
HSSP	HSSP built from PDB template 1L4U based on UniProtKB P95014.
ModBase	Search...
Genome annotation databases
GeneID	1032025.
GenomeReviews	Gene locus CE1741 in contig BA000035_GR.
KEGG	cef:CE1741.
NMPDR	fig|196164.1.peg.1741.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8FT30.
OMA	HANVFFV.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1741-MON.
BRENDA	2.7.1.71. 277326.
Family and domain databases
HAMAP	MF_00109. [Tree]
InterPro	IPR000623. Shik_kinase. [Graphical view]
Pfam	PF01202. SKI. 1 hit. [Graphical view]
PRINTS	PR01100. SHIKIMTKNASE.
PROSITE	PS01128. SHIKIMATE_KINASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROK_COREF

Accession

Primary (citable) accession number: Q8FT30

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)