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Protein

D-inositol 3-phosphate glycosyltransferase

Gene

mshA

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the transfer of a N-acetyl-glucosamine moiety to 1D-myo-inositol 3-phosphate to produce 1D-myo-inositol 2-acetamido-2-deoxy-glucopyranoside 3-phosphate in the mycothiol biosynthesis pathway.UniRule annotation

Catalytic activityi

UDP-N-acetyl-D-glucosamine + 1D-myo-inositol 3-phosphate = UDP + 1-O-(2-acetamido-2-deoxy-alpha-D-glucopyranosyl)-1D-myo-inositol 3-phosphate.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei91D-inositol 3-phosphateUniRule annotation1
Binding sitei23UDP-GlcNAc; via amide nitrogenUniRule annotation1
Binding sitei781D-inositol 3-phosphateUniRule annotation1
Binding sitei1101D-inositol 3-phosphateUniRule annotation1
Binding sitei1341D-inositol 3-phosphateUniRule annotation1
Binding sitei1541D-inositol 3-phosphateUniRule annotation1
Binding sitei231UDP-GlcNAcUniRule annotation1
Binding sitei236UDP-GlcNAcUniRule annotation1
Binding sitei294UDP-GlcNAc; via amide nitrogen and carbonyl oxygenUniRule annotation1
Metal bindingi303Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi304Magnesium; via carbonyl oxygenUniRule annotation1
Metal bindingi306Magnesium; via carbonyl oxygenUniRule annotation1
Binding sitei316UDP-GlcNAcUniRule annotation1
Binding sitei324UDP-GlcNAcUniRule annotation1
Metal bindingi330MagnesiumUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Ligandi

Magnesium, Metal-binding

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Names & Taxonomyi

Protein namesi
Recommended name:
D-inositol 3-phosphate glycosyltransferase (EC:2.4.1.250UniRule annotation)
Alternative name(s):
N-acetylglucosamine-inositol-phosphate N-acetylglucosaminyltransferaseUniRule annotation
Short name:
GlcNAc-Ins-P N-acetylglucosaminyltransferaseUniRule annotation
Gene namesi
Name:mshAUniRule annotation
Ordered Locus Names:CE0422
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001409 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004001171 – 424D-inositol 3-phosphate glycosyltransferaseAdd BLAST424

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi196164.HMPREF0290_2959.

Structurei

3D structure databases

ProteinModelPortaliQ8FSH1.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni15 – 16UDP-GlcNAc bindingUniRule annotation2
Regioni20 – 251D-inositol 3-phosphate bindingUniRule annotation6

Sequence similaritiesi

Belongs to the glycosyltransferase group 1 family. MshA subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FSH1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRVAMISMHT SPLQQPGVGD SGGMNVYILS TGTELARQGV EVDIFTRATR
60 70 80 90 100
PSQGEVVQVA PNLRVINIVA GPYEGLAKEE LSTQLAAFAG GVLEFTRRGG
110 120 130 140 150
IEYDLIHSHY WLSGQVGWLM RDLWRIPLVH TAHTLAAVKN SYRADEDTPE
160 170 180 190 200
SEARRICEQQ LVDNADVLAV NTQEELADLV HHYDADPDRI SVVSPGADIA
210 220 230 240 250
LYTPGNDRAT ERSRRELGVP LHAKVVAFVG RLQPFKGPQV LIHAVAELLE
260 270 280 290 300
RDPQRNLRVL ICGGPSGPSA TPETYRNLAV ELGVDKRIRF LDPRPPEELV
310 320 330 340 350
AVYRAADIIA VPSYNESFGL VAMEAQATGT PVVAARVGGL PVAVAEGETG
360 370 380 390 400
LLVDGHDPAL WADTLATLLD DDETRIRMGQ DAVEHARNFS WAATATQLSS
410 420
LYSEATTAEC DGGIPRRADG ARWD
Length:424
Mass (Da):46,017
Last modified:March 1, 2003 - v1
Checksum:iBAE1EDBDC024E26A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC17232.1.
RefSeqiWP_006770340.1. NZ_GG700690.1.

Genome annotation databases

EnsemblBacteriaiBAC17232; BAC17232; BAC17232.
KEGGicef:CE0422.
PATRICi21486998. VBICorEff9312_0445.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC17232.1.
RefSeqiWP_006770340.1. NZ_GG700690.1.

3D structure databases

ProteinModelPortaliQ8FSH1.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196164.HMPREF0290_2959.

Protein family/group databases

CAZyiGT4. Glycosyltransferase Family 4.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC17232; BAC17232; BAC17232.
KEGGicef:CE0422.
PATRICi21486998. VBICorEff9312_0445.

Phylogenomic databases

eggNOGiENOG4107R5M. Bacteria.
COG0438. LUCA.
HOGENOMiHOG000077288.
KOiK15521.
OMAiHTMAKVK.
OrthoDBiPOG091H0AO3.

Family and domain databases

HAMAPiMF_01695. MshA. 1 hit.
InterProiIPR001296. Glyco_trans_1.
IPR028098. Glyco_trans_4-like_N.
IPR017814. Mycothiol_biosynthesis_MshA.
[Graphical view]
PfamiPF13439. Glyco_transf_4. 1 hit.
PF00534. Glycos_transf_1. 1 hit.
[Graphical view]
TIGRFAMsiTIGR03449. mycothiol_MshA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiMSHA_COREF
AccessioniPrimary (citable) accession number: Q8FSH1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: March 1, 2003
Last modified: November 2, 2016
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.