Q8FSF0 (AROE_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Quinate/shikimate dehydrogenase
EC=1.1.1.-
EC=1.1.1.24

Alternative name(s):
NAD(+)-dependent quinate dehydrogenase
Short name=QDH

Gene names

Name:	aroE
Ordered Locus Names:	CE0443

Organism

Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]

Taxonomic identifier

196164 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium efficiens

Protein attributes

Sequence length	284 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the NAD+-dependent oxidation of both quinate and shikimate to 3-dehydroquinate and 3-dehydroshikimate, respectively. Seems to play a key role in the quinate degradation pathway By similarity. HAMAP-Rule MF_00222
Catalytic activity	Quinate + NAD+ = 3-dehydroquinate + NADH. HAMAP-Rule MF_00222 Shikimate + NAD+ = 3-dehydroshikimate + NADH. HAMAP-Rule MF_00222
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 4/7. HAMAP-Rule MF_00222
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the shikimate dehydrogenase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Ligand	NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP chorismate biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	nucleotide binding Inferred from electronic annotation. Source: InterPro quinate 3-dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: EC shikimate 3-dehydrogenase (NADP+) activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 284	284	Quinate/shikimate dehydrogenase HAMAP-Rule MF_00222		PRO_0000325114
Regions
	Nucleotide binding	135 – 139	5	NAD By similarity
	Nucleotide binding	252 – 256	5	NAD By similarity
Sites
	Active site	74	1	Proton acceptor Potential
	Binding site	111	1	Substrate By similarity
	Binding site	159	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FSF0 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 7179FA02CF0DE537	FASTA	284	29,938
	10 20 30 40 50 60 MNHDSILLGL IGEDISLSRT PAMHEAEGLA QGAATVYRRI DTLTDRARGR SLQELLDAAR 70 80 90 100 110 120 STGFNGLNIT HPYKQAVLPL LDEVSEQAAQ LGAVNTVVIG EDGRTSGHNT DVTGFARGLE 130 140 150 160 170 180 EGLPDATMTT VVQVGAGGVG NAVAYSLVTH GVEQLQVADL DPARAQALAD AINSAIGREA 190 200 210 220 230 240 VHGIDARGVE EAIAAADGVV NATPMGMLAH PGTAFDTSCL TPHHWVGDVV YMPIETQLLK 250 260 270 280 DARALGCRTL DGTRMAIHQA VDAFRLFTGL EPDVERMRAT FLSL

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC17253.1.
RefSeq	NP_737053.1. NC_004369.1.
3D structure databases
HSSP	HSSP built from PDB template 1NVT based on UniProtKB Q58484.
ProteinModelPortal	Q8FSF0.
ModBase	Search...
Protein-protein interaction databases
STRING	196164.CE0443.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC17253; BAC17253; BAC17253.
GeneID	1034899.
KEGG	cef:CE0443.
PATRIC	21487042. VBICorEff9312_0467.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000237875.
KO	K00014.
OMA	HEHEGDA.
ProtClustDB	PRK14027.
Enzyme and pathway databases
UniPathway	UPA00053; UER00087.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
HAMAP	MF_00222. Shikimate_DH_AroE. Divergent sequence.
InterPro	IPR016040. NAD(P)-bd_dom. IPR013708. Shikimate_DH-bd_N. IPR022893. Shikimate_quinate_DH. IPR006151. Shikm_DH/Glu-tRNA_Rdtase. [Graphical view]
Pfam	PF01488. Shikimate_DH. 1 hit. PF08501. Shikimate_dh_N. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROE_COREF

Accession

Primary (citable) accession number: Q8FSF0

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 18, 2008
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program