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Q8FSB6

- MEND_COREF

UniProt

Q8FSB6 - MEND_COREF

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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene
menD, CE0478
Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC) By similarity.UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Magnesium or manganese By similarity.UniRule annotation
Binds 1 thiamine pyrophosphate per subunit By similarity.

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciCEFF196164:GJW8-487-MONOMER.
UniPathwayiUPA00079; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase (EC:2.2.1.9)
Short name:
SEPHCHC synthase
Alternative name(s):
Menaquinone biosynthesis protein MenD
Gene namesi
Name:menD
Ordered Locus Names:CE0478
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001409: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5435432-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotationPRO_0000341727Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi196164.CE0478.

Structurei

3D structure databases

ProteinModelPortaliQ8FSB6.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000218359.
KOiK02551.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FSB6-1 [UniParc]FASTAAdd to Basket

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MSVTPAQELA REVIEALSPH ITDVVLCPGS RNSPLSLEVL SREDLRVHVR    50
IDERSAAFLA LGLARVQRRP VPVIMTSGTA VSNCLPAVTE AAHAHIPLLV 100
VSADRPAHLI GTGSSQTIDQ TDIFGALAPT VTVAAPEHVG RISQALGNGA 150
SQSPRHINVA LDMPLVAPDL PELHGQRGPV DWEQRWVDHG VVDVDLSRNT 200
LVIAGDEAWE IEELAEVPTI AEPTAPVAYH PVHPLAAEFL LKDQVSAEGY 250
VVTTRPDHII VVGHPTLHRG VLKLMTDPTI ELTVLSRTDV ITDPGRHADR 300
VGSRVKVTGE QQKQWLKICD AASDLAAEGV REVLAKEEHG FTGLHVAAAV 350
ADGLGTGDTV FCAASNPIRD LSIVGLPFAG VDVHSPRGTA GIDGSVSQAI 400
GTALAIQARH PDEIRAPRTV ALLGDLAFIH DAGGLLIGPD EPRPENLTIV 450
VANDNGGGIF ELLETGAPGL RSRFERAFGT PHDVNISDLC DAYGVDYRRA 500
NTLQDLLMEL EDTIEIPGFT VIEAVTVRDT RRALQRELTA KVR 543
Length:543
Mass (Da):57,989
Last modified:July 1, 2008 - v2
Checksum:i8462D61D2D35CDF4
GO

Sequence cautioni

The sequence BAC17288.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000035 Genomic DNA. Translation: BAC17288.1. Different initiation.
RefSeqiNP_737088.1. NC_004369.1.

Genome annotation databases

EnsemblBacteriaiBAC17288; BAC17288; BAC17288.
GeneIDi1032597.
KEGGicef:CE0478.
PATRICi21487112. VBICorEff9312_0502.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000035 Genomic DNA. Translation: BAC17288.1 . Different initiation.
RefSeqi NP_737088.1. NC_004369.1.

3D structure databases

ProteinModelPortali Q8FSB6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196164.CE0478.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC17288 ; BAC17288 ; BAC17288 .
GeneIDi 1032597.
KEGGi cef:CE0478.
PATRICi 21487112. VBICorEff9312_0502.

Phylogenomic databases

HOGENOMi HOG000218359.
KOi K02551.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00079 ; UER00164 .
BioCyci CEFF196164:GJW8-487-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view ]
Pfami PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
    Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
    Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Entry informationi

Entry nameiMEND_COREF
AccessioniPrimary (citable) accession number: Q8FSB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: June 11, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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