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Q8FSB6

- MEND_COREF

UniProt

Q8FSB6 - MEND_COREF

Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 71 (01 Oct 2014)
      Sequence version 2 (01 Jul 2008)
      Previous versions | rss
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    Functioni

    Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

    Catalytic activityi

    Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

    Cofactori

    Magnesium or manganese.UniRule annotation
    Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

    Pathwayi

    GO - Molecular functioni

    1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
    2. metal ion binding Source: UniProtKB-KW
    3. thiamine pyrophosphate binding Source: InterPro

    GO - Biological processi

    1. menaquinone biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Menaquinone biosynthesis

    Keywords - Ligandi

    Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

    Enzyme and pathway databases

    BioCyciCEFF196164:GJW8-487-MONOMER.
    UniPathwayiUPA00079; UER00164.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
    Short name:
    SEPHCHC synthaseUniRule annotation
    Alternative name(s):
    Menaquinone biosynthesis protein MenDUniRule annotation
    Gene namesi
    Name:menDUniRule annotation
    Ordered Locus Names:CE0478
    OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
    Taxonomic identifieri196164 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000001409: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 5435432-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthasePRO_0000341727Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi196164.CE0478.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8FSB6.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000218359.
    KOiK02551.
    OrthoDBiEOG6NWBQW.

    Family and domain databases

    Gene3Di3.40.50.970. 2 hits.
    HAMAPiMF_01659. MenD.
    InterProiIPR004433. MenaQ_synth_MenD.
    IPR029061. THDP-binding.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    [Graphical view]
    PfamiPF02776. TPP_enzyme_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF004983. MenD. 1 hit.
    SUPFAMiSSF52518. SSF52518. 2 hits.
    TIGRFAMsiTIGR00173. menD. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8FSB6-1 [UniParc]FASTAAdd to Basket

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    MSVTPAQELA REVIEALSPH ITDVVLCPGS RNSPLSLEVL SREDLRVHVR    50
    IDERSAAFLA LGLARVQRRP VPVIMTSGTA VSNCLPAVTE AAHAHIPLLV 100
    VSADRPAHLI GTGSSQTIDQ TDIFGALAPT VTVAAPEHVG RISQALGNGA 150
    SQSPRHINVA LDMPLVAPDL PELHGQRGPV DWEQRWVDHG VVDVDLSRNT 200
    LVIAGDEAWE IEELAEVPTI AEPTAPVAYH PVHPLAAEFL LKDQVSAEGY 250
    VVTTRPDHII VVGHPTLHRG VLKLMTDPTI ELTVLSRTDV ITDPGRHADR 300
    VGSRVKVTGE QQKQWLKICD AASDLAAEGV REVLAKEEHG FTGLHVAAAV 350
    ADGLGTGDTV FCAASNPIRD LSIVGLPFAG VDVHSPRGTA GIDGSVSQAI 400
    GTALAIQARH PDEIRAPRTV ALLGDLAFIH DAGGLLIGPD EPRPENLTIV 450
    VANDNGGGIF ELLETGAPGL RSRFERAFGT PHDVNISDLC DAYGVDYRRA 500
    NTLQDLLMEL EDTIEIPGFT VIEAVTVRDT RRALQRELTA KVR 543
    Length:543
    Mass (Da):57,989
    Last modified:July 1, 2008 - v2
    Checksum:i8462D61D2D35CDF4
    GO

    Sequence cautioni

    The sequence BAC17288.1 differs from that shown. Reason: Erroneous initiation.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000035 Genomic DNA. Translation: BAC17288.1. Different initiation.
    RefSeqiNP_737088.1. NC_004369.1.

    Genome annotation databases

    EnsemblBacteriaiBAC17288; BAC17288; BAC17288.
    GeneIDi1032597.
    KEGGicef:CE0478.
    PATRICi21487112. VBICorEff9312_0502.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000035 Genomic DNA. Translation: BAC17288.1 . Different initiation.
    RefSeqi NP_737088.1. NC_004369.1.

    3D structure databases

    ProteinModelPortali Q8FSB6.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196164.CE0478.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC17288 ; BAC17288 ; BAC17288 .
    GeneIDi 1032597.
    KEGGi cef:CE0478.
    PATRICi 21487112. VBICorEff9312_0502.

    Phylogenomic databases

    HOGENOMi HOG000218359.
    KOi K02551.
    OrthoDBi EOG6NWBQW.

    Enzyme and pathway databases

    UniPathwayi UPA00079 ; UER00164 .
    BioCyci CEFF196164:GJW8-487-MONOMER.

    Family and domain databases

    Gene3Di 3.40.50.970. 2 hits.
    HAMAPi MF_01659. MenD.
    InterProi IPR004433. MenaQ_synth_MenD.
    IPR029061. THDP-binding.
    IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
    [Graphical view ]
    Pfami PF02776. TPP_enzyme_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF004983. MenD. 1 hit.
    SUPFAMi SSF52518. SSF52518. 2 hits.
    TIGRFAMsi TIGR00173. menD. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
      Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
      Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

    Entry informationi

    Entry nameiMEND_COREF
    AccessioniPrimary (citable) accession number: Q8FSB6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 2008
    Last sequence update: July 1, 2008
    Last modified: October 1, 2014
    This is version 71 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3