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Q8FSB6

- MEND_COREF

UniProt

Q8FSB6 - MEND_COREF

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Protein

2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthase

Gene

menD

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the thiamine diphosphate-dependent decarboxylation of 2-oxoglutarate and the subsequent addition of the resulting succinic semialdehyde-thiamine pyrophosphate anion to isochorismate to yield 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate (SEPHCHC).UniRule annotation

Catalytic activityi

Isochorismate + 2-oxoglutarate = 5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-carboxylate + CO2.UniRule annotation

Cofactori

Magnesium or manganese.UniRule annotation
Binds 1 thiamine pyrophosphate per subunit.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. thiamine pyrophosphate binding Source: InterPro

GO - Biological processi

  1. menaquinone biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Menaquinone biosynthesis

Keywords - Ligandi

Magnesium, Manganese, Metal-binding, Thiamine pyrophosphate

Enzyme and pathway databases

BioCyciCEFF196164:GJW8-487-MONOMER.
UniPathwayiUPA00079.
UPA01057; UER00164.

Names & Taxonomyi

Protein namesi
Recommended name:
2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthaseUniRule annotation (EC:2.2.1.9UniRule annotation)
Short name:
SEPHCHC synthaseUniRule annotation
Alternative name(s):
Menaquinone biosynthesis protein MenDUniRule annotation
Gene namesi
Name:menDUniRule annotation
Ordered Locus Names:CE0478
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001409: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 5435432-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylate synthasePRO_0000341727Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi196164.CE0478.

Structurei

3D structure databases

ProteinModelPortaliQ8FSB6.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the TPP enzyme family. MenD subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000218359.
KOiK02551.
OrthoDBiEOG6NWBQW.

Family and domain databases

Gene3Di3.40.50.970. 2 hits.
HAMAPiMF_01659. MenD.
InterProiIPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view]
PfamiPF02776. TPP_enzyme_N. 1 hit.
[Graphical view]
PIRSFiPIRSF004983. MenD. 1 hit.
SUPFAMiSSF52518. SSF52518. 2 hits.
TIGRFAMsiTIGR00173. menD. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FSB6-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSVTPAQELA REVIEALSPH ITDVVLCPGS RNSPLSLEVL SREDLRVHVR
60 70 80 90 100
IDERSAAFLA LGLARVQRRP VPVIMTSGTA VSNCLPAVTE AAHAHIPLLV
110 120 130 140 150
VSADRPAHLI GTGSSQTIDQ TDIFGALAPT VTVAAPEHVG RISQALGNGA
160 170 180 190 200
SQSPRHINVA LDMPLVAPDL PELHGQRGPV DWEQRWVDHG VVDVDLSRNT
210 220 230 240 250
LVIAGDEAWE IEELAEVPTI AEPTAPVAYH PVHPLAAEFL LKDQVSAEGY
260 270 280 290 300
VVTTRPDHII VVGHPTLHRG VLKLMTDPTI ELTVLSRTDV ITDPGRHADR
310 320 330 340 350
VGSRVKVTGE QQKQWLKICD AASDLAAEGV REVLAKEEHG FTGLHVAAAV
360 370 380 390 400
ADGLGTGDTV FCAASNPIRD LSIVGLPFAG VDVHSPRGTA GIDGSVSQAI
410 420 430 440 450
GTALAIQARH PDEIRAPRTV ALLGDLAFIH DAGGLLIGPD EPRPENLTIV
460 470 480 490 500
VANDNGGGIF ELLETGAPGL RSRFERAFGT PHDVNISDLC DAYGVDYRRA
510 520 530 540
NTLQDLLMEL EDTIEIPGFT VIEAVTVRDT RRALQRELTA KVR
Length:543
Mass (Da):57,989
Last modified:July 1, 2008 - v2
Checksum:i8462D61D2D35CDF4
GO

Sequence cautioni

The sequence BAC17288.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000035 Genomic DNA. Translation: BAC17288.1. Different initiation.
RefSeqiNP_737088.1. NC_004369.1.

Genome annotation databases

EnsemblBacteriaiBAC17288; BAC17288; BAC17288.
GeneIDi1032597.
KEGGicef:CE0478.
PATRICi21487112. VBICorEff9312_0502.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000035 Genomic DNA. Translation: BAC17288.1 . Different initiation.
RefSeqi NP_737088.1. NC_004369.1.

3D structure databases

ProteinModelPortali Q8FSB6.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196164.CE0478.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC17288 ; BAC17288 ; BAC17288 .
GeneIDi 1032597.
KEGGi cef:CE0478.
PATRICi 21487112. VBICorEff9312_0502.

Phylogenomic databases

HOGENOMi HOG000218359.
KOi K02551.
OrthoDBi EOG6NWBQW.

Enzyme and pathway databases

UniPathwayi UPA00079 .
UPA01057 ; UER00164 .
BioCyci CEFF196164:GJW8-487-MONOMER.

Family and domain databases

Gene3Di 3.40.50.970. 2 hits.
HAMAPi MF_01659. MenD.
InterProi IPR004433. MenaQ_synth_MenD.
IPR029061. THDP-binding.
IPR012001. Thiamin_PyroP_enz_TPP-bd_dom.
[Graphical view ]
Pfami PF02776. TPP_enzyme_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF004983. MenD. 1 hit.
SUPFAMi SSF52518. SSF52518. 2 hits.
TIGRFAMsi TIGR00173. menD. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
    Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
    Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Entry informationi

Entry nameiMEND_COREF
AccessioniPrimary (citable) accession number: Q8FSB6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 2008
Last sequence update: July 1, 2008
Last modified: October 29, 2014
This is version 72 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3