Q8FS13 (ALR_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Alanine racemase
EC=5.1.1.1

Gene names

Name:	alr
Ordered Locus Names:	CE0593

Organism

Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]

Taxonomic identifier

196164 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium efficiens

Protein attributes

Sequence length	367 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the interconversion of L-alanine and D-alanine. May also act on other amino acids By similarity. HAMAP-Rule MF_01201
Catalytic activity	L-alanine = D-alanine. HAMAP-Rule MF_01201
Cofactor	Pyridoxal phosphate By similarity. HAMAP-Rule MF_01201
Pathway	Amino-acid biosynthesis; D-alanine biosynthesis; D-alanine from L-alanine: step 1/1. HAMAP-Rule MF_01201
Sequence similarities	Belongs to the alanine racemase family.

Ontologies

Keywords
Ligand	Pyridoxal phosphate
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	D-alanine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Molecular_function	alanine racemase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 367	367	Alanine racemase HAMAP-Rule MF_01201		PRO_1000065983
Sites
	Active site	34	1	Proton acceptor; specific for D-alanine By similarity
	Active site	258	1	Proton acceptor; specific for L-alanine By similarity
	Binding site	131	1	Substrate By similarity
	Binding site	306	1	Substrate; via amide nitrogen By similarity
Amino acid modifications
	Modified residue	34	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FS13 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: B07005E2CF893D6A	FASTA	367	39,352
	10 20 30 40 50 60 MNLLTTRIDL DAIAHNTRLL KNRVGAAKLM AVVKADGYNH GMDRVAPVMA ANGADAFGVA 70 80 90 100 110 120 TIAEALALRA TGITTPILCW IWSPEQDWAA AVEAGIDLAV ISPRHARVLV DAPPTSRAIR 130 140 150 160 170 180 VSVKVDTALH RSGVDEQDWD AVFTLLRDCG HIEVTGLFTH LSCADEPGNP ETDHQAETFR 190 200 210 220 230 240 RAIDRARALG LEVPENHLCN SPATLTRPDL HMDMVRPGVA LYGLEPIPGL DHGLRPAMTW 250 260 270 280 290 300 AGAITVVKPI RKGEGTSYGL TWRAEADGFV AVVPAGYADG VPRAAQDHLR VHVNGHDYPQ 310 320 330 340 350 360 VGRVCMDQFV IFLGDNPHGV TAGDEAVIFG GTGMSATELA DALATINYEV ICRPTGRTVR DYTGEGK

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC17403.1.
RefSeq	NP_737203.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FS13.
SMR	Q8FS13. Positions 1-362.
ModBase	Search...
Protein-protein interaction databases
STRING	196164.CE0593.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC17403; BAC17403; BAC17403.
GeneID	1034900.
KEGG	cef:CE0593.
PATRIC	21487356. VBICorEff9312_0620.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000031444.
KO	K01775.
OMA	NFKSIEI.
ProtClustDB	PRK00053.
Enzyme and pathway databases
UniPathway	UPA00042; UER00497.
Family and domain databases
Gene3D	2.40.37.10. 1 hit.
HAMAP	MF_01201. Ala_racemase.
InterPro	IPR000821. Ala_racemase. IPR009006. Ala_racemase/Decarboxylase_C. IPR011079. Ala_racemase_C. IPR001608. Ala_racemase_N. [Graphical view]
Pfam	PF00842. Ala_racemase_C. 1 hit. PF01168. Ala_racemase_N. 1 hit. [Graphical view]
PRINTS	PR00992. ALARACEMASE.
SMART	SM01005. Ala_racemase_C. 1 hit. [Graphical view]
SUPFAM	SSF50621. Racem_decarbox_C. 1 hit.
TIGRFAMs	TIGR00492. alr. 1 hit.
PROSITE	PS00395. ALANINE_RACEMASE. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ALR_COREF

Accession

Primary (citable) accession number: Q8FS13

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	March 1, 2003
Last modified:	May 29, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program