Q8FRX6 (DNAE2_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Error-prone DNA polymerase
EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	CE0632

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	1073 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm By similarity HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1073	1073	Error-prone DNA polymerase HAMAP MF_01902		PRO_0000103377

Sequences

	Sequence		Length	Mass (Da)
	Q8FRX6 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 827F8754ED71EE32	FASTA	1,073	116,229
	10 20 30 40 50 60 MVGMEWDTGA RGLTGRPLPW SMVEGILSGR EVGTPRPVLH EAEPECLSTP RPGPGSTEVP 70 80 90 100 110 120 GERRGSRQGE RSGEIPFVEL HATSSYNFLA GASDPGEMVD RAHELGLSAL ALVDRDGLYG 130 140 150 160 170 180 AVRFAEAAAE VGLATVFGAE LSLREGVLTV LCRGVEGYRR LSHLITGAAM SAGEKGSVDY 190 200 210 220 230 240 PLLPQVAEQG AGHWVVLAGV EWQKKIDHLI ECFGRDNVVL EFPARMLPED TDHHDILRSI 250 260 270 280 290 300 QTRTGLRGIL STMPTAATRD HVRLAGAKCA LALRANLAEA ESSLHPMGGT WLRSGGALAR 310 320 330 340 350 360 AYPQCGDLLA TTVEIASGCA FTFDLVAPEL PRWDTPDGHT EMTWLTHLVE SRFDRRYRSR 370 380 390 400 410 420 PAEVRERARA QIRHELGVIE QLGFPGYFLI VDDLVQFCHN ATILCQGRGS AANSAVCFVL 430 440 450 460 470 480 GITNAEPITA GLLFERFLSR DRDGPPDIDI DIESGRREEV IQYVYTRYGR DNAAQVANVI 490 500 510 520 530 540 TYRTKGALRD AARALGYPQG TVDAWSRGAS EPPADVVELA GQLKGQPRHL GIHSGGMVIC 550 560 570 580 590 600 DRPIADVVPT EWARMEGRSV VQWDKDDCAA AGLVKFDLLG LGMLEALHHM MDLVAHHRGI 610 620 630 640 650 660 TVNLWELDLA DAGVYDMLCR ADAVGVFQVE SRAQMSTLPR LKPRTFFDLV VEVALIRPGP 670 680 690 700 710 720 IQGGSVHPYL RRRSGEEAVT YDHPVLEKSL GKTLGIPLFQ EQIMQIAVDA AGFTGGEADA 730 740 750 760 770 780 LRRAMGSKRS PTRMAALRSR FYQGLADTHG IIGDTADKLW NKMVAFAAYG FPESHSQSFA 790 800 810 820 830 840 TLVYFSAWFK HHYPAEFCAG LLRAQPMGFY SPQSLIADAR RHGVEILPIS INESGVQADA 850 860 870 880 890 900 PDGHLRLGLD LVKGLGEEAA RRIAEHAPYT SIPDLSRRAD LGVAHIEALA RAGALDCLGV 910 920 930 940 950 960 GRREALWQAG IAATERPGML PGISAIEAPA LPGMSAFELM ATSIAATGVT HDAQPMALLR 970 980 990 1000 1010 1020 AHLDALGVVP ADRLLTDVAD GTRVRIAGVV THRQRPQTAS GVTFLGLEDE TGLMNVMVSP 1030 1040 1050 1060 1070 GLWDRQRVLA RTAKTLIIRG IVQNATGAVN VVADKLEPLP VGEWLSRGSR DFR

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC17442.1.
RefSeq	NP_737242.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FRX6.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1032751.
GenomeReviews	Gene locus CE0632 in contig BA000035_GR.
KEGG	cef:CE0632.
NMPDR	fig|196164.1.peg.632.
PATRIC	21487428. VBICorEff9312_0656.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG734490.
OMA	IDEVEQP.
ProtClustDB	PRK05672.
Enzyme and pathway databases
BioCyc	CEFF196164:CE0632-MONOMER.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR023073. DNA_pol_error_prone. IPR012340. NA-bd_OB-fold. IPR004365. NA-bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K14162.
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
SUPFAM	SSF89550. PHP-like. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_COREF

Accession

Primary (citable) accession number: Q8FRX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 19, 2005
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 57 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program