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Q8FRR3 (SYW_COREF) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 65. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tryptophan--tRNA ligase

EC=6.1.1.2
Alternative name(s):
Tryptophanyl-tRNA synthetase
Short name=TrpRS
Gene names
Name:trpS
Ordered Locus Names:CE0696
OrganismCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]
Taxonomic identifier196164 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length346 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp). HAMAP-Rule MF_00140

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00140

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00140.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processtryptophanyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

tryptophan-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 346346Tryptophan--tRNA ligase HAMAP-Rule MF_00140
PRO_0000136625

Regions

Motif22 – 3110"HIGH" region HAMAP-Rule MF_00140
Motif207 – 2115"KMSKS" region HAMAP-Rule MF_00140

Sites

Binding site2101ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FRR3 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: 872FE798B2848DA6

FASTA34637,888
        10         20         30         40         50         60 
MTSQDNDHTA QTPARVLSGI QPTADSYHLG NYLGAVKQWI DLQNSYDAFY FIPDLHAITV 

        70         80         90        100        110        120 
DQNPEELRAR TVSGAAQLLA LGIDPERSTL FVQSHIPAHA ELSWVLTCLT GFGEASRMTQ 

       130        140        150        160        170        180 
FKDKSTKQGA ERTSAGLFTY PMLMAADILL YRPQLVPVGE DQRQHLELTR TLAERFNNRF 

       190        200        210        220        230        240 
GTVFEVPEGF IPEGASKIYD LQNPTAKMSK SGENPKGLIN LLDDPKVSSK RIKSAVTDND 

       250        260        270        280        290        300 
GVIAFDVANK PGVSNLLVIQ SALTGESVDK LVAGYEGKGY GALKTDTADA LEAFTTPLRA 

       310        320        330        340 
KYDEYMSDRA ELERVLAIGA ERAGEIATKV LAEVYDKIGF LAPRSR 

« Hide

References

[1]"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000035 Genomic DNA. Translation: BAC17506.1.
RefSeqNP_737306.1. NC_004369.1.

3D structure databases

ProteinModelPortalQ8FRR3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196164.CE0696.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC17506; BAC17506; BAC17506.
GeneID1032806.
KEGGcef:CE0696.
PATRIC21487550. VBICorEff9312_0717.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000059940.
KOK01867.
OMAGWGQFKP.
OrthoDBEOG686NJQ.
ProtClustDBPRK00927.

Family and domain databases

Gene3D3.40.50.620. 1 hit.
HAMAPMF_00140_B. Trp_tRNA_synth_B.
InterProIPR002305. aa-tRNA-synth_Ic.
IPR014729. Rossmann-like_a/b/a_fold.
IPR002306. Trp-tRNA-ligase.
IPR024109. Trp-tRNA-ligase_bac-type.
[Graphical view]
PANTHERPTHR10055. PTHR10055. 1 hit.
PfamPF00579. tRNA-synt_1b. 1 hit.
[Graphical view]
PRINTSPR01039. TRNASYNTHTRP.
TIGRFAMsTIGR00233. trpS. 1 hit.
ProtoNetSearch...

Entry information

Entry nameSYW_COREF
AccessionPrimary (citable) accession number: Q8FRR3
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2003
Last modified: February 19, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries