Reviewed, UniProtKB/Swiss-Prot Q8FRJ4 (SAHH_COREF)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	CE0767

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	478 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 478	478	Adenosylhomocysteinase HAMAP MF_00563		PRO_0000116959
Regions
	Region	228 – 396	169	NAD binding By similarity
Sites
	Binding site	57	1	Substrate By similarity
	Binding site	139	1	Substrate By similarity
	Binding site	201	1	Substrate By similarity
	Binding site	231	1	Substrate By similarity
	Binding site	235	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FRJ4-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: A75845A1A3F3A978	FASTA	478	52,777
	10 20 30 40 50 60 MAKVTDFKVA DLSLAEAGRH QIRLAEYEMP GLMQLRREYA EEQPLKGARI AGSIHMTVQT 70 80 90 100 110 120 AVLIETLTAL GAEVRWASCN IFSTQDEAAA AIVVGDGTPE DPQGVPVFAW KGETLDEYWW 130 140 150 160 170 180 CINQIFSWEG ELPNMILDDG GDATMAVIRG REYEKAGVVP QPEANDSDEY IAFLGMLREV 190 200 210 220 230 240 LAEEPDKWTR LADSIKGVTE ETTTGVHRLY HFAEEGVLPF PAMNVNDAVT KSKFDNKYGT 250 260 270 280 290 300 RHSLIDGINR ATDMLMGGKN VLVCGYGDVG KGCAEAFDGQ GARVRVTEAD PINALQALMD 310 320 330 340 350 360 GYSVVTVDEA IADADIVITA TGNKDIISYE QMLKMKDHAL LGNIGHFDNE IDMHSLLHRD 370 380 390 400 410 420 DVIRTTIKPQ VDEFTFPNGK SIIVLSEGRL LNLGNATGHP SFVMSTSFAD QTIAQIELFQ 430 440 450 460 470 NEGQYENQVY RLPKILDEKV ARIHVEALGG KLTELTKEQA EYIGVDVAGP FKPEHYRY

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000035 Genomic DNA. Translation: BAC17577.1.
RefSeq	NP_737377.1.
3D structure databases
HSSP	HSSP built from PDB template 1B3R based on UniProtKB P10760.
ModBase	Search...
Genome annotation databases
GeneID	1032872.
GenomeReviews	Gene locus CE0767 in contig BA000035_GR.
KEGG	cef:CE0767.
NMPDR	fig|196164.1.peg.767.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8FRJ4.
OMA	HMRAMKD.
Enzyme and pathway databases
BioCyc	CEFF196164:CE0767-MON.
BRENDA	3.3.1.1. 277326.
Family and domain databases
HAMAP	MF_00563. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_COREF

Accession

Primary (citable) accession number: Q8FRJ4

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 20, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)