Q8FRI2 (AROA_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-phosphoshikimate 1-carboxyvinyltransferase
EC=2.5.1.19

Alternative name(s):
5-enolpyruvylshikimate-3-phosphate synthase
Short name=EPSP synthase
Short name=EPSPS

Gene names

Name:	aroA
Ordered Locus Names:	CE0779

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	408 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Phosphoenolpyruvate + 3-phosphoshikimate = phosphate + 5-O-(1-carboxyvinyl)-3-phosphoshikimate. HAMAP MF_00210
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 6/7. HAMAP MF_00210
Subunit structure	Monomer By similarity. HAMAP MF_00210
Subcellular location	Cytoplasm By similarity HAMAP MF_00210.
Sequence similarities	Belongs to the EPSP synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-phosphoshikimate 1-carboxyvinyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 408	408	3-phosphoshikimate 1-carboxyvinyltransferase HAMAP MF_00210		PRO_0000088250

Sequences

	Sequence		Length	Mass (Da)
	Q8FRI2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 17971703A8A6A7ED	FASTA	408	43,612
	10 20 30 40 50 60 MWPQVIPGSK SITNRALILA ALAAGPSTIH GVLRSRDTDL MADALRSMGV TITEEAPDRY 70 80 90 100 110 120 HVEPPAELSS GSIDCGLAGT VMRFVPPVAA FADGPVHFDG DEQARIRPMT SILDALRTLG 130 140 150 160 170 180 VRVENNRLPF TVTSDGIPEG GVVEIDASAS SQFVSGLLLS APRFVNGVTV RHIGGRLPSM 190 200 210 220 230 240 PHIEMTVAML REVGIRVDVT PNQWTVHPGE IRGHTWRIEP DLSNATPFLA AAAVTRGTVT 250 260 270 280 290 300 VRNWPRDTTQ PGDSIRSILE DMGCTVEFIS NGATFDLEVT GPAELKGIHL DMSDIGELTP 310 320 330 340 350 360 TVAALAALAT TESRLTGIAH LRGHETNRLE ALTAEINRLG GNCTELDDGL HITPAPLHGG 370 380 390 400 VWHSYADHRM ATAGAIIGLV IKDVGVEDIQ TTSKTFPGFE TLWEEMVG

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC17589.1.
RefSeq	NP_737389.2. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FRI2.
SMR	Q8FRI2. Positions 5-407.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1031943.
GenomeReviews	Gene locus CE0779 in contig BA000035_GR.
KEGG	cef:CE0779.
NMPDR	fig|196164.1.peg.779.
PATRIC	21487718. VBICorEff9312_0801.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG646626.
OMA	HRMATAG.
PhylomeDB	Q8FRI2.
ProtClustDB	PRK02427.
Enzyme and pathway databases
BioCyc	CEFF196164:CE0779-MONOMER.
Family and domain databases
HAMAP	MF_00210. EPSP_synth. [Tree]
InterPro	IPR001986. Enolpyruvate_Tfrase_dom. IPR006264. EPSP_synthase. IPR023193. EPSP_synthase_CS. IPR013792. RNA3'P_cycl/enolpyr_Trfase_a/b. [Graphical view]
Gene3D	G3DSA:3.65.10.10. EPSP_synthase. 2 hits.
KO	K00800.
Pfam	PF00275. EPSP_synthase. 1 hit. [Graphical view]
PIRSF	PIRSF000505. EPSPS. 1 hit.
SUPFAM	SSF55205. RNA3'_cycl/enolpyr_transf_A/B. 1 hit.
TIGRFAMs	TIGR01356. AroA. 1 hit.
PROSITE	PS00104. EPSP_SYNTHASE_1. 1 hit. PS00885. EPSP_SYNTHASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROA_COREF

Accession

Primary (citable) accession number: Q8FRI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program