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Protein

Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase

Gene

CE0987

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the aldol cleavage of 4-hydroxy-4-methyl-2-oxoglutarate (HMG) into 2 molecules of pyruvate. Also contains a secondary oxaloacetate (OAA) decarboxylase activity due to the common pyruvate enolate transition state formed following C-C bond cleavage in the retro-aldol and decarboxylation reactions (By similarity).By similarity

Catalytic activityi

4-hydroxy-4-methyl-2-oxoglutarate = 2 pyruvate.
Oxaloacetate = pyruvate + CO2.

Cofactori

a divalent metal cationBy similarityNote: Divalent metal cation.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei103 – 1031SubstrateBy similarity
Metal bindingi104 – 1041Divalent metal cationBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Ligandi

Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolase (EC:4.1.3.17)
Short name:
HMG aldolase
Alternative name(s):
Oxaloacetate decarboxylase (EC:4.1.1.3)
Short name:
OAA decarboxylase
Regulator of ribonuclease activity homolog
RraA-like protein
Gene namesi
Ordered Locus Names:CE0987
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001409 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 170170Putative 4-hydroxy-4-methyl-2-oxoglutarate aldolasePRO_0000209608Add
BLAST

Interactioni

Subunit structurei

Homotrimer.By similarity

Protein-protein interaction databases

STRINGi196164.HMPREF0290_1469.

Structurei

3D structure databases

ProteinModelPortaliQ8FQY0.
SMRiQ8FQY0. Positions 7-159.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni81 – 844Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the class II aldolase/RraA-like family.Curated

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OrthoDBiEOG6Z3KKK.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FQY0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTDTHGTFIP TADLVDIIGD DVRSCDTQFR NLGGRTDFHG TITTVKCFQD
60 70 80 90 100
NALLKSILGE DNPGGVLVID GDASLHTALV GDIIAGLGRD HGWSGVVVNG
110 120 130 140 150
AIRDSAVIGE MEFGCKALGT NPRKSSKTGA GERDVVVSFG GVDFTPGHYL
160 170
YADSDGIVVT ENPVKAPASN
Length:170
Mass (Da):17,719
Last modified:September 26, 2003 - v2
Checksum:i7696EAE1E320198A
GO

Sequence cautioni

The sequence BAC17797.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC17797.1. Different initiation.
RefSeqiWP_035109947.1. NZ_GG700688.1.

Genome annotation databases

EnsemblBacteriaiBAC17797; BAC17797; BAC17797.
KEGGicef:CE0987.
PATRICi21488118. VBICorEff9312_0995.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC17797.1. Different initiation.
RefSeqiWP_035109947.1. NZ_GG700688.1.

3D structure databases

ProteinModelPortaliQ8FQY0.
SMRiQ8FQY0. Positions 7-159.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196164.HMPREF0290_1469.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC17797; BAC17797; BAC17797.
KEGGicef:CE0987.
PATRICi21488118. VBICorEff9312_0995.

Phylogenomic databases

eggNOGiENOG4108YYX. Bacteria.
COG0684. LUCA.
HOGENOMiHOG000252803.
KOiK02553.
OrthoDBiEOG6Z3KKK.

Family and domain databases

Gene3Di3.50.30.40. 1 hit.
InterProiIPR010203. RraA.
IPR005493. RraA/RraA-like.
[Graphical view]
PfamiPF03737. Methyltransf_6. 1 hit.
[Graphical view]
SUPFAMiSSF89562. SSF89562. 1 hit.
TIGRFAMsiTIGR01935. NOT-MenG. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
    Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
    Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Entry informationi

Entry nameiRRAAH_COREF
AccessioniPrimary (citable) accession number: Q8FQY0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: September 26, 2003
Last modified: April 13, 2016
This is version 68 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.