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Protein

Urease subunit alpha

Gene

ureC

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

Urea + H2O = CO2 + 2 NH3.UniRule annotation

Cofactori

Ni cationUniRule annotationNote: Binds 2 nickel ions per subunit.UniRule annotation

Pathwayi: urea degradation

This protein is involved in step 1 of the subpathway that synthesizes CO(2) and NH(3) from urea (urease route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Urease subunit gamma (ureA), Urease subunit alpha (ureC), Urease subunit beta (ureB)
This subpathway is part of the pathway urea degradation, which is itself part of Nitrogen metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CO(2) and NH(3) from urea (urease route), the pathway urea degradation and in Nitrogen metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi138 – 1381Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi140 – 1401Nickel 1; via tele nitrogenUniRule annotation
Metal bindingi221 – 2211Nickel 1; via carbamate groupUniRule annotation
Metal bindingi221 – 2211Nickel 2; via carbamate groupUniRule annotation
Binding sitei223 – 2231SubstrateUniRule annotation
Metal bindingi250 – 2501Nickel 2; via pros nitrogenUniRule annotation
Metal bindingi276 – 2761Nickel 2; via tele nitrogenUniRule annotation
Active sitei324 – 3241Proton donorUniRule annotation
Metal bindingi364 – 3641Nickel 1UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nickel

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Protein family/group databases

MEROPSiM38.982.

Names & Taxonomyi

Protein namesi
Recommended name:
Urease subunit alphaUniRule annotation (EC:3.5.1.5UniRule annotation)
Alternative name(s):
Urea amidohydrolase subunit alphaUniRule annotation
Gene namesi
Name:ureCUniRule annotation
Ordered Locus Names:CE0995
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001409 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 571571Urease subunit alphaPRO_0000234151Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei221 – 2211N6-carboxylysineUniRule annotation

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two nickel ions.UniRule annotation

Interactioni

Subunit structurei

Heterotrimer of UreA (gamma), UreB (beta) and UreC (alpha) subunits. Three heterotrimers associate to form the active enzyme.UniRule annotation

Protein-protein interaction databases

STRINGi196164.HMPREF0290_1461.

Structurei

3D structure databases

ProteinModelPortaliQ8FQX2.
SMRiQ8FQX2. Positions 3-571.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini133 – 571439UreaseUniRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the urease family.UniRule annotation
Contains 1 urease domain.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiPOG091H0ARR.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 2 hits.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8FQX2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFSMSRKQHA SMYGPTTGDR LRLADTGLFA EIEEDLTIAG EEAVFGGGKV
60 70 80 90 100
VRVGMGQNGH RTREEDPDIP DTVITNVIVL DWSGIYKADI GIRDGIITGI
110 120 130 140 150
GHAGNPDTMD GGTIPIGVST DIIAGEGKVL TAGGIDTHIH FISPEQIEAA
160 170 180 190 200
LVSGITTMIG GGTGPSESTK ATTITPGPWH IHQMLRSLDG FPMNIGLLGK
210 220 230 240 250
GHATASAPLI DQIRAGAIGL KVHEDWGATP ATIDLSLSVA DEFDIQVAVH
260 270 280 290 300
TDTLNEAGFV ESTREAIGGR VIHTFHTEGA GGGHAPDIIA LAGEPNVLPA
310 320 330 340 350
STNPTLPYTV NTVEEHLDML MVCHHLNPDV PEDVAFADSR IRTETIAAED
360 370 380 390 400
VLHDMGIFSI TSSDSQAMGR VGEVIIRTWQ VADSMKRQRG PLEGDTADSD
410 420 430 440 450
NNRIKRYVAK YTINPALAQG ISHVVGSVET GKFADLVLWE PKFFGVKPFM
460 470 480 490 500
ILKGGQVVNS IMGDAGASIP TPQPELYRPM FGSYGSATSS NSITFLPRVA
510 520 530 540 550
IAAGIPEQLG LNRRIEECHG IRTLTKQSLK LNGETPSIHV DPETYEVRID
560 570
GALITCEPAE KLPLAQRYFL F
Length:571
Mass (Da):60,969
Last modified:March 1, 2003 - v1
Checksum:i55C06EF074E7B7F8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC17805.1.

Genome annotation databases

EnsemblBacteriaiBAC17805; BAC17805; BAC17805.
KEGGicef:CE0995.
PATRICi21488134. VBICorEff9312_1003.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC17805.1.

3D structure databases

ProteinModelPortaliQ8FQX2.
SMRiQ8FQX2. Positions 3-571.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196164.HMPREF0290_1461.

Protein family/group databases

MEROPSiM38.982.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC17805; BAC17805; BAC17805.
KEGGicef:CE0995.
PATRICi21488134. VBICorEff9312_1003.

Phylogenomic databases

eggNOGiENOG4105CQM. Bacteria.
COG0804. LUCA.
HOGENOMiHOG000075064.
KOiK01428.
OMAiFDSHIHF.
OrthoDBiPOG091H0ARR.

Enzyme and pathway databases

UniPathwayiUPA00258; UER00370.

Family and domain databases

CDDicd00375. Urease_alpha. 1 hit.
Gene3Di2.30.40.10. 2 hits.
HAMAPiMF_01953. Urease_alpha. 1 hit.
InterProiIPR006680. Amidohydro-rel.
IPR011059. Metal-dep_hydrolase_composite.
IPR032466. Metal_Hydrolase.
IPR011612. Urease_alpha_N_dom.
IPR017950. Urease_AS.
IPR005848. Urease_asu.
IPR017951. Urease_asu_c.
IPR029754. Urease_Ni-bd.
[Graphical view]
PfamiPF01979. Amidohydro_1. 1 hit.
PF00449. Urease_alpha. 1 hit.
[Graphical view]
PRINTSiPR01752. UREASE.
SUPFAMiSSF51338. SSF51338. 2 hits.
SSF51556. SSF51556. 2 hits.
TIGRFAMsiTIGR01792. urease_alph. 1 hit.
PROSITEiPS01120. UREASE_1. 1 hit.
PS00145. UREASE_2. 1 hit.
PS51368. UREASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiURE1_COREF
AccessioniPrimary (citable) accession number: Q8FQX2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 2, 2006
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.