Q8FQV2 (KPRS_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribose-phosphate pyrophosphokinase
Short name=RPPK
EC=2.7.6.1

Alternative name(s):
Phosphoribosyl pyrophosphate synthase
Short name=P-Rib-PP synthase
Short name=PRPP synthase

Gene names

Name:	prs
Ordered Locus Names:	CE1015

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	325 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + D-ribose 5-phosphate = AMP + 5-phospho-alpha-D-ribose 1-diphosphate. HAMAP MF_00583_B
Cofactor	Binds 1 magnesium ion per subunit By similarity. HAMAP MF_00583_B
Pathway	Metabolic intermediate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate biosynthesis; 5-phospho-alpha-D-ribose 1-diphosphate from D-ribose 5-phosphate (route I): step 1/1. HAMAP MF_00583_B
Subcellular location	Cytoplasm By similarity HAMAP MF_00583_B.
Sequence similarities	Belongs to the ribose-phosphate pyrophosphokinase family.

Ontologies

Keywords
Biological process	Nucleotide biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleoside metabolic process Inferred from electronic annotation. Source: InterPro ribonucleoside monophosphate biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW kinase activity Inferred from electronic annotation. Source: UniProtKB-KW magnesium ion binding Inferred from electronic annotation. Source: InterPro ribose phosphate diphosphokinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 325	325	Ribose-phosphate pyrophosphokinase HAMAP MF_00583_B		PRO_0000141130
Regions
	Region	223 – 236	14	Binding of phosphoribosylpyrophosphate Potential
Sites
	Metal binding	136	1	Magnesium Potential
	Metal binding	138	1	Magnesium Potential
	Metal binding	147	1	Magnesium Potential
	Metal binding	151	1	Magnesium Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8FQV2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: CEB4A938B27BB079	FASTA	325	35,567
	10 20 30 40 50 60 MTAHWKENHK NLMLFSGRAH PELAEAVARD LEVEVTPMTA RDFANGEIYV RFEESVRGSD 70 80 90 100 110 120 CFVIQSHTQP LNKWLMEQLL MIDALKRGSA KRITAILPFY PYARQDKKHR GREPISARLI 130 140 150 160 170 180 ADLLQTAGAD RIVSVDLHTD QIQGFFDGPV DHMHAMPILT DYIKDKYDLS NIVVVSPDAG 190 200 210 220 230 240 RVKVAEKWAN TLGDAPMAFV HKTRSTEVAN EVVANRVVGD VAGKDCVLLD DMIDTGGTIA 250 260 270 280 290 300 GAVGVLKEAG ARSVVIACTH GVFSDPARER LSSCGAEEVI TTDTLPQSTE GWSNLTVLPI 310 320 APLLARTISE IFENGSVTTL FEGDA

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC17825.1.
RefSeq	NP_737625.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FQV2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1033179.
GenomeReviews	Gene locus CE1015 in contig BA000035_GR.
KEGG	cef:CE1015.
NMPDR	fig|196164.1.peg.1015.
PATRIC	21488168. VBICorEff9312_1020.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG519284.
OMA	CATHAVF.
PhylomeDB	Q8FQV2.
ProtClustDB	PRK03092.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1015-MONOMER.
Family and domain databases
HAMAP	MF_00583_B. RibP_PPkinase_B. [Tree]
InterPro	IPR000842. PRib_PP_synth_CS. IPR000836. PRibTrfase. IPR005946. Rib-P_diPkinase. [Graphical view]
KO	K00948.
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01251. RibP_PPkin. 1 hit.
PROSITE	PS00114. PRPP_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KPRS_COREF

Accession

Primary (citable) accession number: Q8FQV2

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 3, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program