Reviewed, UniProtKB/Swiss-Prot Q8FQP8 (FUMC_COREF)

Names and origin

Protein names

Recommended name:
    Fumarate hydratase class II
      Short name=Fumarase C
    EC=4.2.1.2

Gene names

Name:	fumC
Ordered Locus Names:	CE1071

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	473 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	(S)-malate = fumarate + H2O. HAMAP MF_00743
Pathway	Carbohydrate metabolism; tricarboxylic acid cycle. HAMAP MF_00743
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Miscellaneous	There are 2 substrate binding sites: the catalytic A site, and the non-catalytic B site that may play a role in the transfer of substrate or product between the active site and the solvent. Alternatively, the B site may bind allosteric effectors By similarity.
Sequence similarities	Belongs to the class-II fumarase/aspartase family. Fumarase subfamily.

Ontologies

Keywords
Biological process	Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tricarboxylic acid cycle Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	fumarate hydratase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 473	473	Fumarate hydratase class II HAMAP MF_00743		PRO_0000161271
Regions
	Region	128 – 131	4	B site By similarity
	Region	138 – 140	3	Substrate binding By similarity
Sites
	Binding site	106	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FQP8-1 [UniParc]. Last modified December 15, 2003. Version 2. Checksum: C4CC4FFA6F3EA1A3	FASTA	473	50,073
	10 20 30 40 50 60 MSDFMTEQEF RIEHDTMGEV KVPAQALWRA QTQRAVENFP ISGRGLESAQ IRAMGLLKAA 70 80 90 100 110 120 CAQVNKDSGA LDAAKADAII AAGKEIATGK HDAEFPIDVF QTGSGTSSNM NTNEVIASIA 130 140 150 160 170 180 KANGTEVHPN DDVNMGQSSN DTFPTATHVA ATEAAVNDLI PGLKVLHESL AKKANEWDSV 190 200 210 220 230 240 VKSGRTHLMD AVPVTLGQEF GGYARQIQLG IERIEATLPR LGELAIGGTA VGTGINTSAD 250 260 270 280 290 300 FGGKVVAELV ELTGVTQLQE AANHFEAQAN RDALVEFSGA MRVVAVSLYK IANDIRLMGS 310 320 330 340 350 360 GPLTGLGEIQ LPDLQPGSSI MPGKVNPVLC ETATQVSAQV IGNDAAVAFA GSQGQFELNV 370 380 390 400 410 420 FIPVMARNVL ESARLLANTA RVFATRLVDG IVPNEEHMKQ LAESSPSIVT PLNSAIGYEA 430 440 450 460 470 AAKVAKAALA EGKTIRQTVI DMGFVDGEKL TEEELDKRLD VLAMANTDRK QKF

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000035 Genomic DNA. Translation: BAC17881.1. Different initiation.
RefSeq	NP_737681.2.
3D structure databases
HSSP	HSSP built from PDB template 1YFM based on UniProtKB P08417.
ModBase	Search...
Genome annotation databases
GeneID	1033250.
GenomeReviews	Gene locus CE1071 in contig BA000035_GR.
KEGG	cef:CE1071.
NMPDR	fig|196164.1.peg.1071.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8FQP8.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1071-MON.
BRENDA	4.2.1.2. 277326.
Family and domain databases
HAMAP	MF_00743. [Tree]
InterPro	IPR003031. D_crystallin. IPR018951. Fumarase_C_C. IPR000362. Fumarate_lyase. IPR020557. Fumarate_lyase_CS. [Graphical view]
Pfam	PF10415. FumaraseC_C. 1 hit. PF00206. Lyase_1. 1 hit. [Graphical view]
PRINTS	PR00145. ARGSUCLYASE. PR00149. FUMRATELYASE.
PROSITE	PS00163. FUMARATE_LYASES. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FUMC_COREF

Accession

Primary (citable) accession number: Q8FQP8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	December 15, 2003
Last modified:	September 22, 2009
This is version 39 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)