Q8FQB2 (METE_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase
EC=2.1.1.14

Alternative name(s):
Cobalamin-independent methionine synthase
Methionine synthase, vitamin-B12 independent isozyme

Gene names

Name:	metE
Ordered Locus Names:	CE1209

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	754 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the transfer of a methyl group from 5-methyltetrahydrofolate to homocysteine resulting in methionine formation By similarity. HAMAP MF_00172
Catalytic activity	5-methyltetrahydropteroyltri-L-glutamate + L-homocysteine = tetrahydropteroyltri-L-glutamate + L-methionine. HAMAP MF_00172
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00172
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-methionine from L-homocysteine (MetE route): step 1/1. HAMAP MF_00172
Sequence similarities	Belongs to the vitamin-B12 independent methionine synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Domain	Repeat
Ligand	Metal-binding Zinc
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	5-methyltetrahydropteroyltriglutamate-homocysteine S-methyltransferase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 754	754	5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase HAMAP MF_00172		PRO_0000098626
Sites
	Metal binding	633	1	Zinc By similarity
	Metal binding	635	1	Zinc By similarity
	Metal binding	718	1	Zinc By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FQB2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 3235983CE12A2A9A	FASTA	754	82,384
	10 20 30 40 50 60 MTSFFSSTIA GVPRIGAHRE LKFALEGFWS GTVSGRELGQ TATTLTNDYC DQLAAAGLDS 70 80 90 100 110 120 IPTAGRSYYD AMLDTTALLG VFPERFQNLD DHPNDGLPAH IDRYFATARG TAELPASAMT 130 140 150 160 170 180 KWFDTNYHYL VPELSADTRF ILDDTALLTD IHDQLTRGHD VRPVLIGPLT YLSLSRTTDG 190 200 210 220 230 240 SDPLDHLETL FEVFERLLAK LPTPWVQLDE PSLVTDVAPE VLERVRAGYA RLATRGGVFV 250 260 270 280 290 300 NTYFGAGDQA LDTLAGLGLG AIGVDLVSDG VTALEAWKGE ELLVAGIVDG RNVWRTDLCA 310 320 330 340 350 360 ALGTLRRLQA RGPVAVSTSC SLLHVPYALT AETGLNPEVR EWLAFGEEKV REVVALADAL 370 380 390 400 410 420 AGEINESLFD AAAAALADRR HSVLTAARDA DITDADRSRS PFEVRAAAQD EALDLPPLPT 430 440 450 460 470 480 TTIGSFPQTR EIRSARARYR AGHLTPEQYE DAMRAEIAQV IRAQEDLGLD VLVHGEPERN 490 500 510 520 530 540 DMVQYFAELL DGFLTTTNGW VQSYGSRCVR PPILFGTVTR PEPMTVRWFA HAQSLTNRPV 550 560 570 580 590 600 KGMLTGPVTI LAWSFVRDDQ PLAVTADQIA LALREEITDL VDAGAKIIQV DEPAIRELLP 610 620 630 640 650 660 LRRAEQDAYT RWAVGAFRLS TSTAPDHVQI HTHMCYSEFN ELIRSIIDLD ADVTTIEAAR 670 680 690 700 710 720 SDMQVLAALK SSGFHLGVGP GVWDIHSPRV PELAEVSQLL SSALESVDPR RLWVNPDCGL 730 740 750 KTRGWEETTA SLKVLVAAAE QARTDLLQRA STSA

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC18019.1.
RefSeq	NP_737819.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FQB2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1033441.
GenomeReviews	Gene locus CE1209 in contig BA000035_GR.
KEGG	cef:CE1209.
NMPDR	fig|196164.1.peg.1209.
PATRIC	21488548. VBICorEff9312_1208.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287495.
OMA	RNIWRAN.
PhylomeDB	Q8FQB2.
ProtClustDB	PRK05222.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1209-MONOMER.
Family and domain databases
HAMAP	MF_00172. Meth_synth. [Tree]
InterPro	IPR013215. Cbl-indep_Met_Synth_N. IPR006276. Cobalamin-indep_Met_synthase. IPR002629. Methionine_synth. [Graphical view]
KO	K00549.
Pfam	PF08267. Meth_synt_1. 1 hit. PF01717. Meth_synt_2. 1 hit. [Graphical view]
PIRSF	PIRSF000382. MeTrfase_B12_ind. 1 hit.
TIGRFAMs	TIGR01371. Met_syn_B12ind. 1 hit.
ProtoNet	Search...

Entry information

Entry name

METE_COREF

Accession

Primary (citable) accession number: Q8FQB2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 56 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program