Q8FQ12 (GLGB_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-alpha-glucan branching enzyme GlgB
EC=2.4.1.18

Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase
Alpha-(1->4)-glucan branching enzyme
Glycogen branching enzyme
Short name=BE

Gene names

Name:	glgB
Ordered Locus Names:	CE1323

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	731 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity. HAMAP MF_00685
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity. HAMAP MF_00685
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen biosynthesis Glycogen metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 731	731	1,4-alpha-glucan branching enzyme GlgB HAMAP MF_00685		PRO_0000188698
Sites
	Active site	408	1	Nucleophile By similarity
	Active site	461	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FQ12 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 63B5690B96EDBB4E	FASTA	731	82,714
	10 20 30 40 50 60 MTPASANDLL IPEEDLNRLR HCHHHNPHGF YGWHATDDGS VIRTRQIGAE KVELVLGDTQ 70 80 90 100 110 120 IVMNPIGDDI FAIKLGNREA FDYRLRVTWP GQDPVVTADP YIFLPTLGEM DTYLISEGRH 130 140 150 160 170 180 ERLWDVLGAN VKTYETTLGQ VRGTAFAVWA PNAIGVAVIG GFNGWNASQH AMRSLGGSGI 190 200 210 220 230 240 WELFIPNIGP GEVYKFAIQT REGHRRDKAD PMARLAELPP ATGSIVVESD YQWQDSEWMD 250 260 270 280 290 300 KRAEIDTATT PMSVYEVHLG SWRWGRSYAE LATELVDYVA DLGYTHVEFM PVAEHPFGGS 310 320 330 340 350 360 WGYQVSGYYA PTSRWGSPDE LRKLIDAFHA RGIGVIIDWV PAHFPKDDWA LARFDGQALY 370 380 390 400 410 420 EHPDWRRGEQ KDWGTYVFNF GRSEVRNFLV ANALYWLEEF HVDGLRVDAV ASMLYLDYSR 430 440 450 460 470 480 EHGEWEPNVY GGRENLEAVQ FLQEMNATVQ RVHPGALTIA EESTSWPGVT APTWDGGLGF 490 500 510 520 530 540 SLKWNMGWMN DTLEYFSKDP IHRSFHHNEL TFSLVYAFSE RFVLPISHDE VVHGKGSLWN 550 560 570 580 590 600 RMPGDTWNKA AGMRTLLAYM WAHPGKKLLF MGQEIGQRDE WSEAHELPWG VVEGWQGEYH 610 620 630 640 650 660 EGISDLVREL NSTYKEVTAL HQRDFSGEGF TWNKADDASN NILVFTRHGD DGSQALCVFN 670 680 690 700 710 720 LSGTSQPEYQ IGVSGGGSWR LVLNTDDEQY HGANNPLPET IEAEKIDRDG FPYTTTLHSP 730 AMSAQFYVWE G

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC18133.1.
RefSeq	NP_737933.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FQ12.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1033641.
GenomeReviews	Gene locus CE1323 in contig BA000035_GR.
KEGG	cef:CE1323.
NMPDR	fig|196164.1.peg.1323.
PATRIC	21488782. VBICorEff9312_1324.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG287139.
OMA	WNTYIFN.
PhylomeDB	Q8FQ12.
ProtClustDB	PRK05402.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1323-MONOMER.
Family and domain databases
HAMAP	MF_00685. GlgB. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz. IPR006048. A-amylase_b_C. IPR015902. Alpha_amylase. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat_dom. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 2 hits.
KO	K00700.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF13. PTHR10357:SF13. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
PIRSF	PIRSF000463. GlgB. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 2 hits.
TIGRFAMs	TIGR01515. Branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB_COREF

Accession

Primary (citable) accession number: Q8FQ12

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 15, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program