Reviewed, UniProtKB/Swiss-Prot Q8FPX6 (ILVD1_COREF)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 1
      Short name=DAD 1
    EC=4.2.1.9

Gene names

Name:	ilvD1
Synonyms:	ilvD
Ordered Locus Names:	CE1362

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	613 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 613	613	Dihydroxy-acid dehydratase 1 HAMAP MF_00012		PRO_0000103461
Sites
	Metal binding	122	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	197	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8FPX6-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 6BBFC87876EA12E9	FASTA	613	64,582
	10 20 30 40 50 60 MIPLRSKVTT VGRNAAGARA LWRATGTKEN EFGKPIVAIV NSYTQFVPGH VHLKNVGDIV 70 80 90 100 110 120 ADAVRAAGGV PKEFNTIAVD DGIAMGHGGM LYSLPSREII ADSVEYMVNA HTADAMVCIS 130 140 150 160 170 180 NCDKITPGML NAAMRLNIPV VFVSGGPMEA GKAVVVDGVA HAPTDLITAI SASANDGVDD 190 200 210 220 230 240 AGLLAVEQSA CPTCGSCSGM FTANSMNCLT EALGLSLPGN GSTLATHSAR RALFEKAGET 250 260 270 280 290 300 VVKLCERYYG QEDESALPRS IATKKAFENA MALDMAMGGS TNTILHILAA AQEGEVDFDL 310 320 330 340 350 360 ADIDALSKRV PCLSKVAPNS DYHMEDVHRA GGIPAILGEL NRGGLLNKDV HSVFSDDLES 370 380 390 400 410 420 WLDEWDIRSG KASEEAIDLF HAAPGGIRTT EAFSTDNRWD SLDTDAENGC IHSIEHAHTA 430 440 450 460 470 480 DGGLVVLRGN IAPDGAVIKS AGIDEELWTF TGPARVVESQ EEAVSVILNK TIQAGEVLVV 490 500 510 520 530 540 RYEGPSGGPG MQEMLHPTAF LKGSGLGKKC ALLTDGRFSG GSSGLSIGHV SPEAAHGGVI 550 560 570 580 590 600 GLIEDGDIIT IDIHNRVLDL GVSDEELERR RAAEEASDKP WQPKNRQRVV SKALRAYAKM 610 ATSADKGAVR QID

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBLGenBankDDBJ	BA000035 Genomic DNA. Translation: BAC18172.1.
RefSeq	NP_737972.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	1033727.
GenomeReviews	Gene locus CE1362 in contig BA000035_GR.
KEGG	cef:CE1362.
NMPDR	fig|196164.1.peg.1362.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q8FPX6.
OMA	SRKVPCL.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1362-MON.
BRENDA	4.2.1.9. 277326.
Family and domain databases
HAMAP	MF_00012. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
ProDom	PD002691. ILVD_EDD_family. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD1_COREF

Accession

Primary (citable) accession number: Q8FPX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 25, 2003
Last sequence update:	March 1, 2003
Last modified:	November 3, 2009
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)