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Q8FPQ9 (DDL_COREF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:CE1431
OrganismCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]
Taxonomic identifier196164 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 361361D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_0000177811

Regions

Domain149 – 353205ATP-grasp
Nucleotide binding176 – 23156ATP By similarity

Sites

Metal binding3081Magnesium or manganese 1 By similarity
Metal binding3201Magnesium or manganese 1 By similarity
Metal binding3201Magnesium or manganese 2 By similarity
Metal binding3221Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FPQ9 [UniParc].

Last modified March 1, 2003. Version 1.
Checksum: BAE7835C7E1EFA1E

FASTA36138,964
        10         20         30         40         50         60 
MSNSNSAKVR VAVLYGGRSA EHSVSCVSAG AIIAHLDPEK YEVIPVGITT DGAWVVGESD 

        70         80         90        100        110        120 
PERLRLVDRT MPEVQRREEI RPSLDPAHRG EFHFADGSLY ATADVIFPVL HGRFGEDGTI 

       130        140        150        160        170        180 
QGMFALSDIP VVGPGVLSSA AGMDKEFTKK LMAAEGLPIG REVILRDRAE LTDAEKKLLG 

       190        200        210        220        230        240 
LPVFVKPARG GSSIGISKVS RWEDLPAAVD LARQHDEKVI VESEIVGPEV ECGVLQYPDG 

       250        260        270        280        290        300 
RIVASLPAML RGTEDGEGGF YDFDTKYLDN VVTAEIPAPL DEEIIELVQS LAVETFQALA 

       310        320        330        340        350        360 
CEGLARVDFF VTANGPVLNE INTMPGFTPI SMYPQMFAAS GVGYEELLDV LVQQALHRSS 


N 

« Hide

References

[1]"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000035 Genomic DNA. Translation: BAC18241.1.
RefSeqNP_738041.1. NC_004369.1.

3D structure databases

ProteinModelPortalQ8FPQ9.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196164.CE1431.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC18241; BAC18241; BAC18241.
GeneID1033870.
KEGGcef:CE1431.
PATRIC21489002. VBICorEff9312_1429.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000011593.
KOK01921.
OMAIECGVLG.
OrthoDBEOG64BQ73.

Enzyme and pathway databases

BioCycCEFF196164:GJW8-1456-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 1 hit.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_COREF
AccessionPrimary (citable) accession number: Q8FPQ9
Entry history
Integrated into UniProtKB/Swiss-Prot: December 15, 2003
Last sequence update: March 1, 2003
Last modified: May 14, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways