true2004-03-012024-01-24103RUVC_COREFComparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens.Nishio Y.Nakamura Y.Kawarabayasi Y.Usuda Y.Kimura E.Sugimoto S.Matsui K.Yamagishi A.Kikuchi H.Ikeo K.Gojobori T.doi:10.1101/gr.12856032003Genome Res.131572-1579NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395Different initiation.BacteriaRuvCRibonuclease H-like superfamily/Ribonuclease HRuvCRNaseH-like_sfRNaseH_sfX-over_junc_endoDNase_Mg_BSX-over_junc_endoDNase_RuvCruvCCROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVCCROSSOVER JUNCTION ENDODEOXYRIBONUCLEASE RUVCRuvCRSOLVASERUVCRibonuclease H-likeRUVCCrossover junction endodeoxyribonuclease RuvC3.1.21.10Holliday junction nuclease RuvCHolliday junction resolvase RuvCruvCCE1775The RuvA-RuvB-RuvC complex processes Holliday junction (HJ) DNA during genetic recombination and DNA repair. Endonuclease that resolves HJ intermediates. Cleaves cruciform DNA by making single-stranded nicks across the HJ at symmetrical positions within the homologous arms, yielding a 5'-phosphate and a 3'-hydroxyl group; requires a central core of homology in the junction. The consensus cleavage sequence is 5'-(A/T)TT(C/G)-3'. Cleavage occurs on the 3'-side of the TT dinucleotide at the point of strand exchange. HJ branch migration catalyzed by RuvA-RuvB allows RuvC to scan DNA until it finds its consensus sequence, where it cleaves and resolves the cruciform DNA.Binds 2 Mg(2+) ion per subunit.Homodimer which binds Holliday junction (HJ) DNA. The HJ becomes 2-fold symmetrical on binding to RuvC with unstacked arms; it has a different conformation from HJ DNA in complex with RuvA. In the full resolvosome a probable DNA-RuvA(4)-RuvB(12)-RuvC(2) complex forms which resolves the HJ.Belongs to the RuvC family.Extended N-terminus.Crossover junction endodeoxyribonuclease RuvC242831223Disordered182Polar residues1862031273146122004-03-0122428397abd233dc5a6ec1e39c0d47f9576639MNHEGLRVMGIDPGLTRCGLSVVQAGRGRTVYPVSVGVVRTPSDAELADRLLRLSQAVGEWMDDYTPDVIAIERVFERGNVSTVMNTAHAVGVLILAAAERGLPVHMYTPSEVKKAISGNGRADKKQMTVMITRILGLAEAPKPADAADALALAVCHCWRAPLLMRTRMTAVDLEHRRRQYQGKLGKAKSTLNARNNAQVTGDAQVRAGHPSQFERPDRADPRtruetrue