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Q8FPK3 (RUVC_COREF) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Crossover junction endodeoxyribonuclease RuvC

EC=3.1.22.4
Alternative name(s):
Holliday junction nuclease RuvC
Holliday junction resolvase RuvC
Gene names
Name:ruvC
Ordered Locus Names:CE1775
OrganismCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]
Taxonomic identifier196164 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length223 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leaves a 5'-terminal phosphate and a 3'-terminal hydroxyl group By similarity. HAMAP-Rule MF_00034

Catalytic activity

Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction). HAMAP-Rule MF_00034

Cofactor

Binds 1 magnesium ion per subunit By similarity. HAMAP-Rule MF_00034

Sequence similarities

Belongs to the RuvC family.

Sequence caution

The sequence BAC18585.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 223223Crossover junction endodeoxyribonuclease RuvC HAMAP-Rule MF_00034
PRO_0000183092

Sites

Metal binding121Magnesium By similarity
Metal binding731Magnesium By similarity
Metal binding1461Magnesium By similarity
Metal binding1491Magnesium By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FPK3 [UniParc].

Last modified March 1, 2004. Version 2.
Checksum: 55D908DF4A284B2C

FASTA22324,283
        10         20         30         40         50         60 
MNHEGLRVMG IDPGLTRCGL SVVQAGRGRT VYPVSVGVVR TPSDAELADR LLRLSQAVGE 

        70         80         90        100        110        120 
WMDDYTPDVI AIERVFERGN VSTVMNTAHA VGVLILAAAE RGLPVHMYTP SEVKKAISGN 

       130        140        150        160        170        180 
GRADKKQMTV MITRILGLAE APKPADAADA LALAVCHCWR APLLMRTRMT AVDLEHRRRQ 

       190        200        210        220 
YQGKLGKAKS TLNARNNAQV TGDAQVRAGH PSQFERPDRA DPR 

« Hide

References

[1]"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000035 Genomic DNA. Translation: BAC18585.1. Different initiation.
RefSeqNP_738385.1. NC_004369.1.

3D structure databases

ProteinModelPortalQ8FPK3.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196164.CE1775.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC18585; BAC18585; BAC18585.
GeneID1032035.
KEGGcef:CE1775.
PATRIC21489687. VBICorEff9312_1764.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000012182.
KOK01159.
OrthoDBEOG6RG044.

Enzyme and pathway databases

BioCycCEFF196164:GJW8-1804-MONOMER.

Family and domain databases

Gene3D3.30.420.10. 1 hit.
HAMAPMF_00034. RuvC.
InterProIPR012337. RNaseH-like_dom.
IPR020563. X-over_junc_endoDNase_Mg_BS.
IPR002176. X-over_junc_endoDNase_RuvC.
[Graphical view]
PfamPF02075. RuvC. 1 hit.
[Graphical view]
PRINTSPR00696. RSOLVASERUVC.
SUPFAMSSF53098. SSF53098. 1 hit.
TIGRFAMsTIGR00228. ruvC. 1 hit.
PROSITEPS01321. RUVC. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRUVC_COREF
AccessionPrimary (citable) accession number: Q8FPK3
Entry history
Integrated into UniProtKB/Swiss-Prot: March 1, 2004
Last sequence update: March 1, 2004
Last modified: May 14, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families