Q8FPI2 (DXS_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-deoxy-D-xylulose-5-phosphate synthase
EC=2.2.1.7

Alternative name(s):
1-deoxyxylulose-5-phosphate synthase
Short name=DXP synthase
Short name=DXPS

Gene names

Name:	dxs
Ordered Locus Names:	CE1796

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	636 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the acyloin condensation reaction between C atoms 2 and 3 of pyruvate and glyceraldehyde 3-phosphate to yield 1-deoxy-D-xylulose-5-phosphate (DXP) By similarity. HAMAP MF_00315
Catalytic activity	Pyruvate + D-glyceraldehyde 3-phosphate = 1-deoxy-D-xylulose 5-phosphate + CO2. HAMAP MF_00315
Cofactor	Binds 1 thiamine pyrophosphate per subunit By similarity.
Pathway	Metabolic intermediate biosynthesis; 1-deoxy-D-xylulose 5-phosphate biosynthesis; 1-deoxy-D-xylulose 5-phosphate from D-glyceraldehyde 3-phosphate and pyruvate: step 1/1. HAMAP MF_00315
Subunit structure	Homodimer By similarity. HAMAP MF_00315
Sequence similarities	Belongs to the transketolase family. DXPS subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis Thiamine biosynthesis
Ligand	Thiamine pyrophosphate
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1-deoxy-D-xylulose-5-phosphate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 636	636	1-deoxy-D-xylulose-5-phosphate synthase HAMAP MF_00315		PRO_0000189108

Sequences

	Sequence		Length	Mass (Da)
	Q8FPI2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 87298D2324BA184B	FASTA	636	67,603
	10 20 30 40 50 60 MGILNSISTP ADLKALAEED LEVLAGEIRS FLVDKVAATG GHLGPNLGVV ELTIGLHRVF 70 80 90 100 110 120 DSPREPIIFD TSHQSYVHKI LTGRGDAFDT LRQKGGLSGY TSRAESEHDW TESSHASAAL 130 140 150 160 170 180 SYADGMSKAF QLDGDTNRNV VAVVGDGALT GGMCWEALNN IAAGKDRNVV VVVNDNGRSY 190 200 210 220 230 240 SPTIGGFAEN LAQLRMEPFY DRVMEQGKST LKSMGWVGER TFEALHAFKE GVKSTVIPTE 250 260 270 280 290 300 MFPELGMKYI GPVNGHDQKA VDNALAYARD FKGPIIVHVV TEKGRGYAPA ERDLDELMHA 310 320 330 340 350 360 TGVMDPRTGI PVAASKPGWT AAFSEELVKL GGRREDIVAI TAAMAGPTGL AKFAANFPSR 370 380 390 400 410 420 FYDVGIAEQH ALTSAAGLAL AGKHPVVAIY STFLNRAFDQ LLMDVALLNL PVTVVLDRSG 430 440 450 460 470 480 VTGSDGASHN GVWDLALTSI VPGIRVAAPR DEDTLRELLG EAVDINDGPS VVRFPKGDLP 490 500 510 520 530 540 APIEAIETLE DGVDILAYLE ASPASEDAPD LLIVAVGERA TLALELGERL VAAGANVTVV 550 560 570 580 590 600 DPRWVIPIPQ SLVALAADHD LVITIEDGVI HGGVGSLLSD ALNAAEVDTP RRQVAVPQKF 610 620 630 LDHASRGQVL LDHDLDIDSV EKTALGWMDR LFGGGN

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC18606.1.
RefSeq	NP_738406.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FPI2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1034417.
GenomeReviews	Gene locus CE1796 in contig BA000035_GR.
KEGG	cef:CE1796.
NMPDR	fig|196164.1.peg.1796.
PATRIC	21489745. VBICorEff9312_1786.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG571647.
OMA	RFANEHD.
ProtClustDB	PRK05444.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1796-MONOMER.
Family and domain databases
HAMAP	MF_00315. DXP_synth. [Tree]
InterPro	IPR005477. Dxylulose-5-P_synthase. IPR009014. Transketo_C/Pyr-ferredox_oxred. IPR015941. Transketolase-like_C. IPR005475. Transketolase-like_Pyr-bd. IPR020826. Transketolase_BS. IPR005476. Transketolase_C. IPR005474. Transketolase_N. [Graphical view]
Gene3D	G3DSA:3.40.50.920. Transketo_C_like. 1 hit.
KO	K01662.
Pfam	PF02779. Transket_pyr. 1 hit. PF02780. Transketolase_C. 1 hit. [Graphical view]
SMART	SM00861. Transket_pyr. 1 hit. [Graphical view]
SUPFAM	SSF52922. Transketo_C_like. 1 hit.
TIGRFAMs	TIGR00204. Dxs. 1 hit.
PROSITE	PS00801. TRANSKETOLASE_1. 1 hit. PS00802. TRANSKETOLASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DXS_COREF

Accession

Primary (citable) accession number: Q8FPI2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program