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Q8FPE1

- DAPF_COREF

UniProt

Q8FPE1 - DAPF_COREF

Protein

Diaminopimelate epimerase

Gene

dapF

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 2 (11 Apr 2003)
      Previous versions | rss
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    Functioni

    Catalyzes the stereoinversion of LL-2,6-diaminoheptanedioate (L,L-DAP) to meso-diaminoheptanedioate (meso-DAP), a precursor of L-lysine and an essential component of the bacterial peptidoglycan.UniRule annotation

    Catalytic activityi

    LL-2,6-diaminoheptanedioate = meso-diaminoheptanedioate.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei15 – 151SubstrateUniRule annotation
    Binding sitei48 – 481SubstrateUniRule annotation
    Binding sitei76 – 761SubstrateUniRule annotation
    Active sitei85 – 851Proton donor/acceptorUniRule annotation
    Binding sitei161 – 1611SubstrateUniRule annotation
    Sitei163 – 1631Important for catalytic activityUniRule annotation
    Binding sitei196 – 1961SubstrateUniRule annotation
    Sitei218 – 2181Important for catalytic activityUniRule annotation
    Active sitei227 – 2271Proton donor/acceptorUniRule annotation

    GO - Molecular functioni

    1. diaminopimelate epimerase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. lysine biosynthetic process via diaminopimelate Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Isomerase

    Keywords - Biological processi

    Amino-acid biosynthesis, Lysine biosynthesis

    Enzyme and pathway databases

    BioCyciCEFF196164:GJW8-1873-MONOMER.
    UniPathwayiUPA00034; UER00025.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Diaminopimelate epimeraseUniRule annotation (EC:5.1.1.7UniRule annotation)
    Short name:
    DAP epimeraseUniRule annotation
    Gene namesi
    Name:dapFUniRule annotation
    Ordered Locus Names:CE1837
    OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
    Taxonomic identifieri196164 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000001409: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 283283Diaminopimelate epimerasePRO_0000149836Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi85 ↔ 227

    Keywords - PTMi

    Disulfide bond

    Interactioni

    Protein-protein interaction databases

    STRINGi196164.CE1837.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8FPE1.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni85 – 873Substrate bindingUniRule annotation
    Regioni218 – 2192Substrate bindingUniRule annotation
    Regioni228 – 2292Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the diaminopimelate epimerase family.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000220467.
    KOiK01778.
    OrthoDBiEOG6ND0M5.

    Family and domain databases

    HAMAPiMF_00197. DAP_epimerase.
    InterProiIPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view]
    PANTHERiPTHR31689. PTHR31689. 1 hit.
    PfamiPF01678. DAP_epimerase. 2 hits.
    [Graphical view]
    TIGRFAMsiTIGR00652. DapF. 1 hit.
    PROSITEiPS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q8FPE1-1 [UniParc]FASTAAdd to Basket

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    MKKEIAFAKG HATENDFIII HDPDGTIDLT ADEVTRLCDR RAGLGADGVL    50
    RVVRAGELGV QTPGVDPGWW FMDYRNADGS LAEMCGNGVR VFAHWLAAHQ 100
    LVDTTDFTIG TRAGARSVTV LSADRDDAVV RVDMGPAAVT GISTCMIADQ 150
    PFAGMGVDLG NPHLACVVPG LTPAALKNIA LEAPVFDTAF FPRGVNVELV 200
    TELADGLDDP AIHMRVWERG VGETRSCGTG TVAAARAALA DAGQAVGTVT 250
    VYVPGGAVEV GIREETSTLT GPSRIIATGT VRI 283
    Length:283
    Mass (Da):29,564
    Last modified:April 11, 2003 - v2
    Checksum:iCF9C66BA14E02F94
    GO

    Sequence cautioni

    The sequence BAC18647.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000035 Genomic DNA. Translation: BAC18647.1. Different initiation.
    RefSeqiNP_738447.1. NC_004369.1.

    Genome annotation databases

    EnsemblBacteriaiBAC18647; BAC18647; BAC18647.
    GeneIDi1034484.
    KEGGicef:CE1837.
    PATRICi21489827. VBICorEff9312_1827.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000035 Genomic DNA. Translation: BAC18647.1 . Different initiation.
    RefSeqi NP_738447.1. NC_004369.1.

    3D structure databases

    ProteinModelPortali Q8FPE1.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196164.CE1837.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC18647 ; BAC18647 ; BAC18647 .
    GeneIDi 1034484.
    KEGGi cef:CE1837.
    PATRICi 21489827. VBICorEff9312_1827.

    Phylogenomic databases

    HOGENOMi HOG000220467.
    KOi K01778.
    OrthoDBi EOG6ND0M5.

    Enzyme and pathway databases

    UniPathwayi UPA00034 ; UER00025 .
    BioCyci CEFF196164:GJW8-1873-MONOMER.

    Family and domain databases

    HAMAPi MF_00197. DAP_epimerase.
    InterProi IPR018510. DAP_epimerase_AS.
    IPR001653. DAP_epimerase_DapF.
    [Graphical view ]
    PANTHERi PTHR31689. PTHR31689. 1 hit.
    Pfami PF01678. DAP_epimerase. 2 hits.
    [Graphical view ]
    TIGRFAMsi TIGR00652. DapF. 1 hit.
    PROSITEi PS01326. DAP_EPIMERASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
      Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
      Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

    Entry informationi

    Entry nameiDAPF_COREF
    AccessioniPrimary (citable) accession number: Q8FPE1
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 11, 2003
    Last sequence update: April 11, 2003
    Last modified: October 1, 2014
    This is version 66 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3