Q8FP63 (RNH2_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribonuclease HII
Short name=RNase HII
EC=3.1.26.4

Gene names

Name:	rnhB
Ordered Locus Names:	CE1925

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	231 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Endonuclease that specifically degrades the RNA of RNA-DNA hybrids By similarity. HAMAP MF_00052_B
Catalytic activity	Endonucleolytic cleavage to 5'-phosphomonoester. HAMAP MF_00052_B
Cofactor	Manganese or magnesium. Binds 1 divalent metal ion per monomer in the absence of substrate. May bind a second metal ion after substrate binding By similarity.
Subcellular location	Cytoplasm Potential HAMAP MF_00052_B.
Sequence similarities	Belongs to the RNase HII family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Manganese Metal-binding
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW ribonuclease H activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 231	231	Ribonuclease HII HAMAP MF_00052_B		PRO_0000111568
Sites
	Metal binding	29	1	Divalent metal cation By similarity
	Metal binding	30	1	Divalent metal cation By similarity
	Metal binding	123	1	Divalent metal cation By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FP63 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F723F97E087BB788	FASTA	231	24,991
	10 20 30 40 50 60 MATIRRLKQL RTYEVALSRH GLGPVAGVDE AGRGACCGPI SIAACILPDR PIADLAVLTD 70 80 90 100 110 120 SKQLSPPVRA RLMPLVKKHA VAWSVIHISA ADIDRFGIQH ANISGMRRAV AALEVRPGYV 130 140 150 160 170 180 LTDAFRIPGL PCPSLPIPGG DASARCIAAA SVLAKQTRDE IMTDMAQQFP QYGLAGHKGY 190 200 210 220 230 STKVHMDAVR RHGASPQHRY SYANVAKAHR EWALSHSDTE VQNTGKVEHE R

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC18735.1.
RefSeq	NP_738535.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FP63.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1034636.
GenomeReviews	Gene locus CE1925 in contig BA000035_GR.
KEGG	cef:CE1925.
NMPDR	fig|196164.1.peg.1925.
PATRIC	21489995. VBICorEff9312_1911.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG584843.
OMA	RLGPTPI.
PhylomeDB	Q8FP63.
ProtClustDB	PRK00015.
Enzyme and pathway databases
BioCyc	CEFF196164:CE1925-MONOMER.
Family and domain databases
HAMAP	MF_00052_B. RNase_HII_B. [Tree]
InterPro	IPR022898. RNase_HII. IPR001352. RNase_HII/HIII. IPR024567. RNase_HII/HIII_dom. IPR012337. RNaseH-like_dom. [Graphical view]
KO	K03470.
PANTHER	PTHR10954. RNase_HII/HIII. 1 hit.
Pfam	PF01351. RNase_HII. 1 hit. [Graphical view]
SUPFAM	SSF53098. RNaseH_fold. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RNH2_COREF

Accession

Primary (citable) accession number: Q8FP63

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program