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Protein

Thiazole synthase

Gene

thiG

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the rearrangement of 1-deoxy-D-xylulose 5-phosphate (DXP) to produce the thiazole phosphate moiety of thiamine. Sulfur is provided by the thiocarboxylate moiety of the carrier protein ThiS. In vitro, sulfur can be provided by H2S.UniRule annotation

Catalytic activityi

1-deoxy-D-xylulose 5-phosphate + 2-iminoacetate + thiocarboxy-adenylate-[sulfur-carrier protein ThiS] = 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate + [sulfur-carrier protein ThiS] + 2 H2O.UniRule annotation

Pathwayi: thiamine diphosphate biosynthesis

This protein is involved in the pathway thiamine diphosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway thiamine diphosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei95 – 951Schiff-base intermediate with DXPUniRule annotation
Binding sitei156 – 1561DXP; via amide nitrogenUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Thiamine biosynthesis

Keywords - Ligandi

Schiff base

Enzyme and pathway databases

UniPathwayiUPA00060.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiazole synthaseUniRule annotation (EC:2.8.1.10UniRule annotation)
Gene namesi
Name:thiGUniRule annotation
Ordered Locus Names:CE1936
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001409 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 259259Thiazole synthasePRO_0000162810Add
BLAST

Interactioni

Subunit structurei

Homotetramer. Forms heterodimers with either ThiH or ThiS.UniRule annotation

Protein-protein interaction databases

STRINGi196164.HMPREF0290_1969.

Structurei

3D structure databases

ProteinModelPortaliQ8FP52.
SMRiQ8FP52. Positions 2-239.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni182 – 1832DXP bindingUniRule annotation
Regioni204 – 2052DXP bindingUniRule annotation

Sequence similaritiesi

Belongs to the ThiG family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiPOG091H01K9.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FP52-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLTIADRTFD SHLIMGTGGA TSMAMLEESL VASGTELTTV AMRRHAATTT
60 70 80 90 100
GESVFDLLRR LDITPLPNTA GCRTARDAVI TAKLAREALG TNWVKVEVIA
110 120 130 140 150
DEHTLLPDVV ELIDACELLV AEGFVVLAYT SDDPVVARRL EDVGCAAVMP
160 170 180 190 200
LGSPIGTGLG ILNPHNIELI CSRAGVPVLL DAGVGTASDA ALAMELGCDG
210 220 230 240 250
VLLASAVNRC QDPVAMARAM RYAVDAGRLA RGAGRIPKRE HAVASSTFDG

LASWSDQVL
Length:259
Mass (Da):27,092
Last modified:March 1, 2003 - v1
Checksum:iADF222E984C0C586
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC18746.1.
RefSeqiWP_006767935.1. NZ_GG700683.1.

Genome annotation databases

EnsemblBacteriaiBAC18746; BAC18746; BAC18746.
KEGGicef:CE1936.
PATRICi21490017. VBICorEff9312_1922.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC18746.1.
RefSeqiWP_006767935.1. NZ_GG700683.1.

3D structure databases

ProteinModelPortaliQ8FP52.
SMRiQ8FP52. Positions 2-239.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196164.HMPREF0290_1969.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC18746; BAC18746; BAC18746.
KEGGicef:CE1936.
PATRICi21490017. VBICorEff9312_1922.

Phylogenomic databases

eggNOGiENOG4105CA8. Bacteria.
COG2022. LUCA.
HOGENOMiHOG000248049.
KOiK03149.
OMAiAQYPSPA.
OrthoDBiPOG091H01K9.

Enzyme and pathway databases

UniPathwayiUPA00060.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_00443. ThiG. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR008867. ThiG.
[Graphical view]
SUPFAMiSSF110399. SSF110399. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiTHIG_COREF
AccessioniPrimary (citable) accession number: Q8FP52
Entry historyi
Integrated into UniProtKB/Swiss-Prot: June 16, 2003
Last sequence update: March 1, 2003
Last modified: September 7, 2016
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.