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Q8FP04 (KPYK_COREF) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Pyruvate kinase

Short name=PK
EC=2.7.1.40
Gene names
Name:pyk
Ordered Locus Names:CE1989
OrganismCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]
Taxonomic identifier196164 [NCBI]
Taxonomic lineageBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium

Protein attributes

Sequence length474 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactor

Magnesium By similarity.

Potassium By similarity.

Pathway

Carbohydrate degradation; glycolysis; pyruvate from D-glyceraldehyde 3-phosphate: step 5/5.

Subunit structure

Homotetramer By similarity.

Sequence similarities

Belongs to the pyruvate kinase family.

Sequence caution

The sequence BAC18799.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 474474Pyruvate kinase
PRO_0000112067

Sites

Metal binding351Potassium By similarity
Metal binding371Potassium By similarity
Metal binding671Potassium By similarity
Metal binding2201Magnesium By similarity
Metal binding2441Magnesium By similarity
Binding site331Substrate By similarity
Binding site2431Substrate; via amide nitrogen By similarity
Binding site2441Substrate; via amide nitrogen By similarity
Binding site2761Substrate By similarity
Site2181Transition state stabilizer By similarity

Sequences

Sequence LengthMass (Da)Tools
Q8FP04 [UniParc].

Last modified January 16, 2004. Version 2.
Checksum: AC0E634D219A5BCE

FASTA47451,504
        10         20         30         40         50         60 
MERRTKIVCT LGPAVASADG ILRLVQDGMD VARLNFSHGD HPDHEQNYKW VREATDKTGR 

        70         80         90        100        110        120 
AVGILADLQG PKIRLGRFKE GSTVWETGET VRITVDDVEG THDRVSTTYK NLAKDARPGD 

       130        140        150        160        170        180 
RLLVDDGKVG LVCVSVEGND VICEVTEGGP VSNNKGVSLP GMDISVPALS EKDIKDLRFA 

       190        200        210        220        230        240 
LKLGVDFIAL SFVRSPADVE LVHAIMDEEG RRVPVIAKLE KPEAVAALES IVLAFDAIMV 

       250        260        270        280        290        300 
ARGDLGVEVP LEEVPLVQKR AIQIARENAK PVIVATQMLD SMIENSRPTR AEASDVANAV 

       310        320        330        340        350        360 
LDGADAVMLS GETSVGKDPH NVVRTMSRIV RFAETDGRVP DLTHIPRTKR GVISYSARDI 

       370        380        390        400        410        420 
AERLNARALV AFTTSGDTAK RLARLHSHLP LLVFTPDPSV RSQLALTWGA QTFLCPKVDD 

       430        440        450        460        470 
TDGMMREVDR ALLAMDEYQK DDMMVVVAGS PPGVTGNTNM IHVHLLGEDT RIPK 

« Hide

References

[1]"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000035 Genomic DNA. Translation: BAC18799.1. Different initiation.
RefSeqNP_738599.1. NC_004369.1.

3D structure databases

ProteinModelPortalQ8FP04.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING196164.CE1989.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAC18799; BAC18799; BAC18799.
GeneID1032053.
KEGGcef:CE1989.
PATRIC21490111. VBICorEff9312_1970.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMHOG000021558.
KOK00873.
OrthoDBEOG6GBMB0.
ProtClustDBPRK06247.

Enzyme and pathway databases

BioCycCEFF196164:GJW8-2025-MONOMER.
UniPathwayUPA00109; UER00188.

Family and domain databases

Gene3D2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERPTHR11817. PTHR11817. 1 hit.
PfamPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSPR01050. PYRUVTKNASE.
SUPFAMSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsTIGR01064. pyruv_kin. 1 hit.
PROSITEPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameKPYK_COREF
AccessionPrimary (citable) accession number: Q8FP04
Entry history
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: November 13, 2013
This is version 73 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways