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Protein

Pyruvate kinase

Gene

pyk

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + pyruvate = ADP + phosphoenolpyruvate.

Cofactori

Protein has several cofactor binding sites:
  • Mg2+By similarity
  • K+By similarity

Pathwayi: glycolysis

This protein is involved in step 5 of the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate.
Proteins known to be involved in the 5 steps of the subpathway in this organism are:
  1. no protein annotated in this organism
  2. Phosphoglycerate kinase (pgk)
  3. 2,3-bisphosphoglycerate-dependent phosphoglycerate mutase (gpmA)
  4. Enolase (eno)
  5. Pyruvate kinase (pyk)
This subpathway is part of the pathway glycolysis, which is itself part of Carbohydrate degradation.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes pyruvate from D-glyceraldehyde 3-phosphate, the pathway glycolysis and in Carbohydrate degradation.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei33SubstrateBy similarity1
Metal bindingi35PotassiumBy similarity1
Metal bindingi37PotassiumBy similarity1
Metal bindingi67PotassiumBy similarity1
Sitei218Transition state stabilizerBy similarity1
Metal bindingi220MagnesiumBy similarity1
Binding sitei243Substrate; via amide nitrogenBy similarity1
Metal bindingi244MagnesiumBy similarity1
Binding sitei244Substrate; via amide nitrogenBy similarity1
Binding sitei276SubstrateBy similarity1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycolysis

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium, Pyruvate

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Names & Taxonomyi

Protein namesi
Recommended name:
Pyruvate kinase (EC:2.7.1.40)
Short name:
PK
Gene namesi
Name:pyk
Ordered Locus Names:CE1989
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
Proteomesi
  • UP000001409 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001120671 – 474Pyruvate kinaseAdd BLAST474

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi196164.HMPREF0290_2021.

Structurei

3D structure databases

ProteinModelPortaliQ8FP04.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the pyruvate kinase family.Curated

Phylogenomic databases

eggNOGiENOG4105CA9. Bacteria.
COG0469. LUCA.
HOGENOMiHOG000021558.
KOiK00873.
OrthoDBiPOG091H02AT.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8FP04-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERRTKIVCT LGPAVASADG ILRLVQDGMD VARLNFSHGD HPDHEQNYKW
60 70 80 90 100
VREATDKTGR AVGILADLQG PKIRLGRFKE GSTVWETGET VRITVDDVEG
110 120 130 140 150
THDRVSTTYK NLAKDARPGD RLLVDDGKVG LVCVSVEGND VICEVTEGGP
160 170 180 190 200
VSNNKGVSLP GMDISVPALS EKDIKDLRFA LKLGVDFIAL SFVRSPADVE
210 220 230 240 250
LVHAIMDEEG RRVPVIAKLE KPEAVAALES IVLAFDAIMV ARGDLGVEVP
260 270 280 290 300
LEEVPLVQKR AIQIARENAK PVIVATQMLD SMIENSRPTR AEASDVANAV
310 320 330 340 350
LDGADAVMLS GETSVGKDPH NVVRTMSRIV RFAETDGRVP DLTHIPRTKR
360 370 380 390 400
GVISYSARDI AERLNARALV AFTTSGDTAK RLARLHSHLP LLVFTPDPSV
410 420 430 440 450
RSQLALTWGA QTFLCPKVDD TDGMMREVDR ALLAMDEYQK DDMMVVVAGS
460 470
PPGVTGNTNM IHVHLLGEDT RIPK
Length:474
Mass (Da):51,504
Last modified:January 16, 2004 - v2
Checksum:iAC0E634D219A5BCE
GO

Sequence cautioni

The sequence BAC18799 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC18799.1. Different initiation.
RefSeqiWP_006767987.1. NZ_GG700683.1.

Genome annotation databases

EnsemblBacteriaiBAC18799; BAC18799; BAC18799.
KEGGicef:CE1989.
PATRICi21490111. VBICorEff9312_1970.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC18799.1. Different initiation.
RefSeqiWP_006767987.1. NZ_GG700683.1.

3D structure databases

ProteinModelPortaliQ8FP04.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196164.HMPREF0290_2021.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC18799; BAC18799; BAC18799.
KEGGicef:CE1989.
PATRICi21490111. VBICorEff9312_1970.

Phylogenomic databases

eggNOGiENOG4105CA9. Bacteria.
COG0469. LUCA.
HOGENOMiHOG000021558.
KOiK00873.
OrthoDBiPOG091H02AT.

Enzyme and pathway databases

UniPathwayiUPA00109; UER00188.

Family and domain databases

Gene3Di2.40.33.10. 1 hit.
3.20.20.60. 2 hits.
3.40.1380.20. 1 hit.
InterProiIPR001697. Pyr_Knase.
IPR015813. Pyrv/PenolPyrv_Kinase-like_dom.
IPR011037. Pyrv_Knase-like_insert_dom.
IPR015794. Pyrv_Knase_a/b.
IPR018209. Pyrv_Knase_AS.
IPR015793. Pyrv_Knase_brl.
IPR015795. Pyrv_Knase_C.
IPR015806. Pyrv_Knase_insert_dom.
[Graphical view]
PANTHERiPTHR11817. PTHR11817. 1 hit.
PfamiPF00224. PK. 1 hit.
PF02887. PK_C. 1 hit.
[Graphical view]
PRINTSiPR01050. PYRUVTKNASE.
SUPFAMiSSF50800. SSF50800. 1 hit.
SSF51621. SSF51621. 2 hits.
SSF52935. SSF52935. 1 hit.
TIGRFAMsiTIGR01064. pyruv_kin. 1 hit.
PROSITEiPS00110. PYRUVATE_KINASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiKPYK_COREF
AccessioniPrimary (citable) accession number: Q8FP04
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 16, 2004
Last sequence update: January 16, 2004
Last modified: November 2, 2016
This is version 87 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.