Q8FNZ7 (HIS4_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase
EC=5.3.1.16

Alternative name(s):
Phosphoribosylformimino-5-aminoimidazole carboxamide ribotide isomerase

Gene names

Name:	hisA
Ordered Locus Names:	CE1996

Organism

Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395) [Complete proteome] [HAMAP]

Taxonomic identifier

196164 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Actinobacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium › Corynebacterium efficiens

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	1-(5-phosphoribosyl)-5-((5-phosphoribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxyribulos-1-ylamino)methylideneamino)-1-(5-phosphoribosyl)imidazole-4-carboxamide. HAMAP-Rule MF_01014
Pathway	Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 4/9. HAMAP-Rule MF_01014
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the HisA/HisF family.
Caution	Ala-129 is present instead of the conserved Asp which is expected to be an active site residue.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Histidine biosynthesis
Cellular component	Cytoplasm
Molecular function	Isomerase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	histidine biosynthetic process Inferred from electronic annotation. Source: HAMAP tryptophan biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino]imidazole-4-carboxamide isomerase activity Inferred from electronic annotation. Source: HAMAP phosphoribosylanthranilate isomerase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)methylideneamino] imidazole-4-carboxamide isomerase HAMAP-Rule MF_01014		PRO_0000142000
Sites
	Active site	10	1	Proton acceptor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FNZ7 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: EF62454178C09B50	FASTA	246	26,624
	10 20 30 40 50 60 MTFTILPAVD VVNGQAVRLD QGEAGTEKSY GTPLESALRW QEQGAEWLHF VDLDAAFNRG 70 80 90 100 110 120 SNHELMAEIT RQLDIKVELT GGIRDDASLE RALATGATRV NIGTAALEKP EWIADVIRRH 130 140 150 160 170 180 GEKIAVDIAV RLENGEWRTK GNGWVSDGGD LWEVLERLDS QGCSRFVVTD VSKDGTLTGP 190 200 210 220 230 240 NVDLLRDVAA ATDAPIVASG GISTLEDVLG LAKYQDEGID SVIIGKALYE HRFTLAEALE AVEKLG

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC18806.1.
RefSeq	NP_738606.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FNZ7.
SMR	Q8FNZ7. Positions 5-241.
ModBase	Search...
Protein-protein interaction databases
STRING	196164.CE1996.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	BAC18806; BAC18806; BAC18806.
GeneID	1032056.
KEGG	cef:CE1996.
PATRIC	21490125. VBICorEff9312_1977.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HOG000224614.
KO	K01814.
OMA	CARYVVT.
ProtClustDB	PRK14024.
Enzyme and pathway databases
UniPathway	UPA00031; UER00009.
Family and domain databases
Gene3D	3.20.20.70. 1 hit.
HAMAP	MF_01014. HisA. Divergent sequence.
InterPro	IPR013785. Aldolase_TIM. IPR006062. His_biosynth. IPR010188. HisA_TrpF. IPR023016. Isoase_HisA. IPR011060. RibuloseP-bd_barrel. [Graphical view]
Pfam	PF00977. His_biosynth. 1 hit. [Graphical view]
SUPFAM	SSF51366. RibP_bind_barrel. 1 hit.
TIGRFAMs	TIGR01919. hisA-trpF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

HIS4_COREF

Accession

Primary (citable) accession number: Q8FNZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 6, 2003
Last sequence update:	March 1, 2003
Last modified:	May 1, 2013
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program