Q8FNW8 (Q8FNW8_COREF) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
Malto-oligosyltrehalose trehalohydrolase PIRNR PIRNR006337
Short name=MTHase PIRNR PIRNR006337
EC=3.2.1.141 PIRNR PIRNR006337

Alternative name(s):
4-alpha-D-((1->4)-alpha-D-glucano)trehalose trehalohydrolase PIRNR PIRNR006337
Maltooligosyl trehalose trehalohydrolase PIRNR PIRNR006337

Gene names

Ordered Locus Names:

CE2025

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	579 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Hydrolysis of (1->4)-alpha-D-glucosidic linkage in 4-alpha-D-((1->4)-alpha-D-glucanosyl)(n) trehalose to yield trehalose and (1->4)-alpha-D-glucan. PIRNR PIRNR006337
Pathway	Glycan biosynthesis; trehalose biosynthesis. PIRNR PIRNR006337
Subcellular location	Cytoplasm By similarity PIRSR PIRSR006337-1.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family. PIRNR PIRNR006337

Ontologies

Keywords
Molecular function	Glycosidase PIRNR PIRNR006337 Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	trehalose biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	4-alpha-D-{(1->4)-alpha-D-glucano}trehalose trehalohydrolase activity Inferred from electronic annotation. Source: EC cation binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Sites
	Active site	255	1	Nucleophile By similarity PIRSR PIRSR006337-1
	Active site	292	1	Proton donor By similarity PIRSR PIRSR006337-1
	Site	388	1	Transition state stabilizer By similarity PIRSR PIRSR006337-3

Sequences

	Sequence		Length	Mass (Da)
	Q8FNW8 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 9FDD9CFC59CB9DF0	FASTA	579	64,334
	10 20 30 40 50 60 MVSLVSMLTS LKYRVWAPRP EDVRLHLNGE TLPMTRTDGD WWVADREPAV GDRYGFELFD 70 80 90 100 110 120 GTEWSKTVPD PRSQYQPDGV HGLSEVISPD FAWSDDAWTG RILPGSVIYE LHVGTFSPSG 130 140 150 160 170 180 DFDGVIERLD YLKDLGVTAV ELMPVQPFGG NRNWGYDGVL WHAVHSGYGG PVGLKKLVDA 190 200 210 220 230 240 CHNAGIAVYL DVVYNHFGPD GNYNGMFAPY TAGGSTGWGE VVNINGPESD EVRRYILDAV 250 260 270 280 290 300 TRWFEEFHID GLRLDAVHSL DDNGAFPLLE QMTVASEAIA AATGIPRSLI AESDLNDPRL 310 320 330 340 350 360 VTSREAGGYG LHAQWVDDIH HALHTLVSGE RNGYYSDFGD TATLASTLEK VFHHTGQVST 370 380 390 400 410 420 FRGRRHGREI RRDVTPASRF VTYTTTHDQT GNRAVGDRPS MNLTPAQQML KAAVIYCSPY 430 440 450 460 470 480 TPMLFMGEEY GATTPFAFFC SHTDDELNRL TSEGRKREFA RLGWDPETIP TPSEESTFTD 490 500 510 520 530 540 SKLDWSFTPE QENILEGYRT LLRLRRELGM SQPDLLSMEI SNGPGWISMA NGDGILVANF 550 560 570 SDSDVEVPFG GELIYSFTSP EVREDSTVLD AYGFAVLRR

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC18835.1.
RefSeq	NP_738635.1. NC_004369.1.
3D structure databases
HSSP	HSSP built from PDB template 1EH9 based on UniProtKB Q55088.
ProteinModelPortal	Q8FNW8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1034765.
GenomeReviews	Gene locus CE2025 in contig BA000035_GR.
KEGG	cef:CE2025.
NMPDR	fig|196164.1.peg.2025.
PATRIC	21490183. VBICorEff9312_2006.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG367595.
OMA	EGFVYQG.
PhylomeDB	Q8FNW8.
ProtClustDB	CLSK638715.
Family and domain databases
InterPro	IPR015902. Alpha_amylase. IPR006047. Glyco_hydro_13_cat_dom. IPR013781. Glyco_hydro_subgr_catalytic. IPR017853. Glycoside_hydrolase_SF. IPR013783. Ig-like_fold. IPR014756. Ig_E-set. IPR012768. Trehalose_TreZ. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 2 hits. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
KO	K01236.
PANTHER	PTHR10357. Alpha_amylase. 1 hit. PTHR10357:SF21. PTHR10357:SF21. 1 hit.
Pfam	PF00128. Alpha-amylase. 2 hits. [Graphical view]
PIRSF	PIRSF006337. Trehalose_TreZ. 1 hit.
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit. SSF81296. Ig_E-set. 1 hit.
TIGRFAMs	TIGR02402. Trehalose_TreZ. 1 hit.
ProtoNet	Search...

Entry information

Entry name

Q8FNW8_COREF

Accession

Primary (citable) accession number: Q8FNW8

Entry history

Integrated into UniProtKB/TrEMBL:	March 1, 2003
Last sequence update:	March 1, 2003
Last modified:	December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)