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Q8FNV0

- SYI_COREF

UniProt

Q8FNV0 - SYI_COREF

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Protein

Isoleucine--tRNA ligase

Gene
ileS, CE2043
Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zinc By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei630 – 6301ATP By similarity

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCEFF196164:GJW8-2079-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CE2043
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001409: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10541054Isoleucine--tRNA ligaseUniRule annotationPRO_0000098537Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi196164.CE2043.

Structurei

3D structure databases

ProteinModelPortaliQ8FNV0.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionUniRule annotationAdd
BLAST
Motifi627 – 6315"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000246403.
KOiK01870.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8FNV0-1 [UniParc]FASTAAdd to Basket

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MTEAVGGVYP LVDMTGGSSR FPEMEENVLD FWKRDDTFQA SIDQRNDAED     50
YVFYDGPPFA NGLPHYGHLL TGYVKDIVPR YQTMRGYRVP RVFGWDTHGL 100
PAELEAEKQL GIKDKGEIES MGLAQFNDYC AKSVLQYTKE WEEYVTRQAR 150
WVDFENGYKT MDLTYMESVI WAFKELYDKG LIYQGFRVLP YSWAEHTPLS 200
NQETRLDDSY KMRQDPTLTV TMPITGKIEG TGANEALIGA NAIAWTTTPW 250
TLPSNLALAV NPSVTYALVE VAGDGEEGFV GQQLLLARDL VGAYAKELGT 300
DARIISEHPG TELIGLTYEP VFDFFRDHPN AFQILGADYV TTEDGTGIVH 350
QAPAFGEDDM NVCNAADIAP VIPVDMDGKF TSLTPEYEGQ LVFDANKDII 400
RDLKAKGRVF RHQTIEHSYP HSWRSGEPLI YMALPSWFVN VTKIRDRMVE 450
TNQDIEWMPA HIRDGQFGKW LEGARDWNIS RSRYWGSPIP VWVSDDENYP 500
RVDVYGSLDE LEADFGVRPT SLHRPYIDEL TRPNPDDPTG KSTMRRVPDV 550
LDVWFDSGSM PFAQVHYPFE NKDWFDTHAP ADFIVEYIGQ TRGWFYLLHV 600
LSVGLFDRPA FKKVVAHGIV LGDDGLKMSK SKGNYPNVTE VFDRDGSDAM 650
RWFLMSSPIL RGGNLIVTEK GIREGVRQAQ LPMWNAYSFL QLYASKKATW 700
SVDSTDVLDR YILAKLHDLV ADVTAALDAT DIARACDQVR WFCDALTNWY 750
VRRSRDRFWA GDEAHPEAFN TLYTVLETLT RVAAPLLPMT TEVIWRGLTG 800
ERSVHLTDFP SADSFPADAD LVRTMDEIRG VCSAASSIRK AHKLRNRLPL 850
PNLTVALPDS GRLADFLSII RDEVNVKNVD LTSDVDAVGT FEVVVNAKVA 900
GPRLGKDVQR VIKAVKAGNY ERVGETVVAD GIELQDGEYT ERLVAANPDS 950
TAQIDDVDGL VVLDMEVTPE LEAEGWAADV IRGLQDARKS SGFEVSDRIQ 1000
VTLSVPGDKQ EWATRHADHI AGEVLATSFE VTQDDLGEDA HEVLKGVTAS 1050
VARV 1054
Length:1,054
Mass (Da):118,226
Last modified:December 20, 2005 - v2
Checksum:i17924A0E38AF555F
GO

Sequence cautioni

The sequence BAC18853.1 differs from that shown. Reason: Erroneous initiation.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000035 Genomic DNA. Translation: BAC18853.1. Different initiation.
RefSeqiNP_738653.2. NC_004369.1.

Genome annotation databases

EnsemblBacteriaiBAC18853; BAC18853; BAC18853.
GeneIDi1034786.
KEGGicef:CE2043.
PATRICi21490221. VBICorEff9312_2025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000035 Genomic DNA. Translation: BAC18853.1 . Different initiation.
RefSeqi NP_738653.2. NC_004369.1.

3D structure databases

ProteinModelPortali Q8FNV0.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 196164.CE2043.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC18853 ; BAC18853 ; BAC18853 .
GeneIDi 1034786.
KEGGi cef:CE2043.
PATRICi 21490221. VBICorEff9312_2025.

Phylogenomic databases

HOGENOMi HOG000246403.
KOi K01870.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CEFF196164:GJW8-2079-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02003. Ile_tRNA_synth_type2.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view ]
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
    Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
    Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Entry informationi

Entry nameiSYI_COREF
AccessioniPrimary (citable) accession number: Q8FNV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: May 14, 2014
This is version 75 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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