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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei630 – 6301ATPUniRule annotation

GO - Molecular functioni

  1. aminoacyl-tRNA editing activity Source: InterPro
  2. ATP binding Source: UniProtKB-HAMAP
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCEFF196164:GJW8-2079-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CE2043
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001409: Chromosome

Subcellular locationi

Cytoplasm UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 10541054Isoleucine--tRNA ligasePRO_0000098537Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi196164.CE2043.

Structurei

3D structure databases

ProteinModelPortaliQ8FNV0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi58 – 6811"HIGH" regionAdd
BLAST
Motifi627 – 6315"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 2 subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000246403.
KOiK01870.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q8FNV0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTEAVGGVYP LVDMTGGSSR FPEMEENVLD FWKRDDTFQA SIDQRNDAED
60 70 80 90 100
YVFYDGPPFA NGLPHYGHLL TGYVKDIVPR YQTMRGYRVP RVFGWDTHGL
110 120 130 140 150
PAELEAEKQL GIKDKGEIES MGLAQFNDYC AKSVLQYTKE WEEYVTRQAR
160 170 180 190 200
WVDFENGYKT MDLTYMESVI WAFKELYDKG LIYQGFRVLP YSWAEHTPLS
210 220 230 240 250
NQETRLDDSY KMRQDPTLTV TMPITGKIEG TGANEALIGA NAIAWTTTPW
260 270 280 290 300
TLPSNLALAV NPSVTYALVE VAGDGEEGFV GQQLLLARDL VGAYAKELGT
310 320 330 340 350
DARIISEHPG TELIGLTYEP VFDFFRDHPN AFQILGADYV TTEDGTGIVH
360 370 380 390 400
QAPAFGEDDM NVCNAADIAP VIPVDMDGKF TSLTPEYEGQ LVFDANKDII
410 420 430 440 450
RDLKAKGRVF RHQTIEHSYP HSWRSGEPLI YMALPSWFVN VTKIRDRMVE
460 470 480 490 500
TNQDIEWMPA HIRDGQFGKW LEGARDWNIS RSRYWGSPIP VWVSDDENYP
510 520 530 540 550
RVDVYGSLDE LEADFGVRPT SLHRPYIDEL TRPNPDDPTG KSTMRRVPDV
560 570 580 590 600
LDVWFDSGSM PFAQVHYPFE NKDWFDTHAP ADFIVEYIGQ TRGWFYLLHV
610 620 630 640 650
LSVGLFDRPA FKKVVAHGIV LGDDGLKMSK SKGNYPNVTE VFDRDGSDAM
660 670 680 690 700
RWFLMSSPIL RGGNLIVTEK GIREGVRQAQ LPMWNAYSFL QLYASKKATW
710 720 730 740 750
SVDSTDVLDR YILAKLHDLV ADVTAALDAT DIARACDQVR WFCDALTNWY
760 770 780 790 800
VRRSRDRFWA GDEAHPEAFN TLYTVLETLT RVAAPLLPMT TEVIWRGLTG
810 820 830 840 850
ERSVHLTDFP SADSFPADAD LVRTMDEIRG VCSAASSIRK AHKLRNRLPL
860 870 880 890 900
PNLTVALPDS GRLADFLSII RDEVNVKNVD LTSDVDAVGT FEVVVNAKVA
910 920 930 940 950
GPRLGKDVQR VIKAVKAGNY ERVGETVVAD GIELQDGEYT ERLVAANPDS
960 970 980 990 1000
TAQIDDVDGL VVLDMEVTPE LEAEGWAADV IRGLQDARKS SGFEVSDRIQ
1010 1020 1030 1040 1050
VTLSVPGDKQ EWATRHADHI AGEVLATSFE VTQDDLGEDA HEVLKGVTAS

VARV
Length:1,054
Mass (Da):118,226
Last modified:December 20, 2005 - v2
Checksum:i17924A0E38AF555F
GO

Sequence cautioni

The sequence BAC18853.1 differs from that shown. Reason: Erroneous initiation. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC18853.1. Different initiation.
RefSeqiNP_738653.2. NC_004369.1.

Genome annotation databases

EnsemblBacteriaiBAC18853; BAC18853; BAC18853.
GeneIDi1034786.
KEGGicef:CE2043.
PATRICi21490221. VBICorEff9312_2025.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC18853.1. Different initiation.
RefSeqiNP_738653.2. NC_004369.1.

3D structure databases

ProteinModelPortaliQ8FNV0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196164.CE2043.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC18853; BAC18853; BAC18853.
GeneIDi1034786.
KEGGicef:CE2043.
PATRICi21490221. VBICorEff9312_2025.

Phylogenomic databases

HOGENOMiHOG000246403.
KOiK01870.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCEFF196164:GJW8-2079-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02003. Ile_tRNA_synth_type2.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023586. Ile-tRNA-ligase_type2.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
    Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
    Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Entry informationi

Entry nameiSYI_COREF
AccessioniPrimary (citable) accession number: Q8FNV0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: December 20, 2005
Last sequence update: December 20, 2005
Last modified: January 7, 2015
This is version 79 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.