Reviewed, UniProtKB/Swiss-Prot Q8FNT5 (MURE_COREF)

Names and origin

Protein names

Recommended name:
    UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase
    EC=6.3.2.13
Alternative name(s):
    UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase
    Meso-diaminopimelate-adding enzyme
    Meso-A2pm-adding enzyme
    UDP-N-acetylmuramyl-tripeptide synthetase
    UDP-MurNAc-tripeptide synthetase

Gene names

Name:	murE
Ordered Locus Names:	CE2058

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	526 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan By similarity. HAMAP MF_00208
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate + meso-2,6-diaminoheptanedioate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-gamma-glutamyl-meso-2,6-diamino-heptanedioate. HAMAP MF_00208
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00208
Subcellular location	Cytoplasm By similarity HAMAP MF_00208.
Post-translational modification	Carbamoylation is probably crucial for Mg2+ binding and, consequently, for the gamma-phosphate positioning of ATP By similarity. HAMAP MF_00208
Sequence similarities	Belongs to the murCDEF family. MurE subfamily.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: HAMAP regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP UDP-N-acetylmuramoylalanyl-D-glutamate-2,6-diaminopimelate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 526	526	UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,6-diaminopimelate ligase HAMAP MF_00208		PRO_0000101889
Regions
	Nucleotide binding	136 – 142	7	ATP Potential
	Region	178 – 179	2	UDP-MurNAc-L-Ala-D-Glu binding By similarity
	Region	432 – 435	4	Meso-diaminopimelate binding By similarity
	Motif	432 – 435	4	Meso-diaminopimelate recognition motif HAMAP MF_00208
Sites
	Binding site	48	1	UDP-MurNAc-L-Ala-D-Glu; via carbonyl oxygen By similarity
	Binding site	50	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	205	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	213	1	UDP-MurNAc-L-Ala-D-Glu By similarity
	Binding site	408	1	Meso-diaminopimelate By similarity
	Binding site	490	1	Meso-diaminopimelate; via carbonyl oxygen By similarity
	Binding site	494	1	Meso-diaminopimelate By similarity
Amino acid modifications
	Modified residue	245	1	N6-carboxylysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FNT5-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 7C7C2B82CB11F577	FASTA	526	55,720
	10 20 30 40 50 60 MCADTPHHPT PVHTEEAMAI TLGELARTIG GELFNAPDQD LEIHNIGLDS STLAKKAPIF 70 80 90 100 110 120 AAVPGSRKHG AEFADTDRAA GALAVLTDRD GATILDRKGD TRPRLIVDDV REVLGVASSS 130 140 150 160 170 180 VYGDPSRDLV LIGVTGTSGK TTTSYLLERG LMEAGYKVGL IGTTGTRIDG EPVPTKLTTP 190 200 210 220 230 240 EAPTLQKLFY RMRQHGVTHV VMEVSSHALS LGRVSGSHFN VAAFTNLSQD HLDFHDTMEE 250 260 270 280 290 300 YFDAKALFFR AGSPLAADHQ VVCIDDEWGT RMAEVAGNAQ TVATTGKRAD FTAGLVTVKE 310 320 330 340 350 360 TGEQSFTVTV PDHGEVPVTL ALPGAFNVAN ATLALAAAVR AGVDPVAFAR GMAQVAVPGR 370 380 390 400 410 420 MERIDEGQDF LAVVDYAHKP AAVAAVLDTL RTQIDGRLGV VIGAGGDRDA TKRAPMGELS 430 440 450 460 470 480 ARRADLVIVT DDNPRSEVPA TIRAAVMEGA RRGAAEADHE VEVREIGDRA EAIRALIEWA 490 500 510 520 GPGDGIVVAG KGHEVGQLIA GVTHHFDDRE QVREALRHTQ SDGEGK

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC18868.1.
RefSeq	NP_738668.1.
3D structure databases
SMR	Q8FNT5. Positions 30-517.
ModBase	Search...
Genome annotation databases
GeneID	1032069.
GenomeReviews	Gene locus CE2058 in contig BA000035_GR.
KEGG	cef:CE2058.
NMPDR	fig|196164.1.peg.2058.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG602753.
OMA	HTPDGIE.
Enzyme and pathway databases
BioCyc	CEFF196164:CE2058-MONOMER.
BRENDA	6.3.2.13. 277326.
Family and domain databases
HAMAP	MF_00208. MurE. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR000713. Mur_ligase_N. IPR005761. UDP-N-AcMur-Glu-dNH2Pim_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit.
Pfam	PF01225. Mur_ligase. 1 hit. PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
TIGRFAMs	TIGR01085. murE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURE_COREF

Accession

Primary (citable) accession number: Q8FNT5

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)