Q8FNT2 (RSMH_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA small subunit methyltransferase H
EC=2.1.1.199

Alternative name(s):
16S rRNA m(4)C1402 methyltransferase
rRNA (cytosine-N(4)-)-methyltransferase RsmH

Gene names

Name:	rsmH
Synonyms:	mraW
Ordered Locus Names:	CE2061

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	338 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the N4 position of cytidine in position 1402 (C1402) of 16S rRNA By similarity. HAMAP MF_01007
Catalytic activity	S-adenosyl-L-methionine + cytosine(1402) in 16S rRNA = S-adenosyl-L-homocysteine + N(4)-methylcytosine(1402) in 16S rRNA. HAMAP MF_01007
Subcellular location	Cytoplasm By similarity HAMAP MF_01007.
Sequence similarities	Belongs to the methyltransferase superfamily. RsmH family.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	rRNA processing Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 338	338	Ribosomal RNA small subunit methyltransferase H HAMAP MF_01007		PRO_0000108614
Regions
	Region	45 – 47	3	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	64	1	S-adenosyl-L-methionine By similarity
	Binding site	91	1	S-adenosyl-L-methionine; via amide nitrogen By similarity
	Binding site	120	1	S-adenosyl-L-methionine By similarity
	Binding site	127	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FNT2 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 1BCF03B897A61F28	FASTA	338	37,119
	10 20 30 40 50 60 MDDYSIAGNH GHVPVMRDRM AALIADHVRE MGDRAVIVDA TLGAGGHAEH FLTVFPEAHV 70 80 90 100 110 120 IGLDRDENAL RDAGERLARF GDRFLGVKTR FDGLSQVLAT GEGDILDLAR KEGISGALFD 130 140 150 160 170 180 LGVSSMQLDQ VERGFAYRTD APLDMRMDPT QGITAADILN TYSHGDIARV LKTYGDERFA 190 200 210 220 230 240 GKIASAVLKE REKEPFTTSA RLVELLYDTI PAATRRTGGH PAKRTFQALR VEVNRELESL 250 260 270 280 290 300 SNVLPQITDA LNVGGRAVFM SYQSHEDKLV KRFFTELTTS KTPPGLPVDL PGTAPRFRQV 310 320 330 TRGAETASEA EIEENPRAAP VKVRAIERIA EHTENTEN

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC18871.1.
RefSeq	NP_738671.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FNT2.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1034796.
GenomeReviews	Gene locus CE2061 in contig BA000035_GR.
KEGG	cef:CE2061.
NMPDR	fig|196164.1.peg.2061.
PATRIC	21490257. VBICorEff9312_2043.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG302779.
OMA	GHVPVMR.
PhylomeDB	Q8FNT2.
ProtClustDB	PRK00050.
Enzyme and pathway databases
BioCyc	CEFF196164:CE2061-MONOMER.
Family and domain databases
HAMAP	MF_01007. 16SrRNA_methyltr_H. [Tree]
InterPro	IPR002903. SAM-dep_MeTrfase_MraW. IPR023397. SAM-dep_MeTrfase_MraW_recog. [Graphical view]
Gene3D	G3DSA:1.10.150.170. G3DSA:1.10.150.170. 1 hit.
KO	K03438.
PANTHER	PTHR11265. Bact_methyltrans. 1 hit.
Pfam	PF01795. Methyltransf_5. 1 hit. [Graphical view]
PIRSF	PIRSF004486. MraW. 1 hit.
SUPFAM	SSF81799. SSF81799. 1 hit.
TIGRFAMs	TIGR00006. TIGR00006. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RSMH_COREF

Accession

Primary (citable) accession number: Q8FNT2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 23, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program