SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

Q8FNB3

- MASZ_COREF

UniProt

Q8FNB3 - MASZ_COREF

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Malate synthase G
Gene
glcB, masZ, CE2231
Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA By similarity.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Magnesium By similarity.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei141 – 1411Acetyl-CoA; via carbonyl oxygen By similarity
Binding sitei298 – 2981Acetyl-CoA By similarity
Binding sitei335 – 3351Acetyl-CoA By similarity
Active sitei362 – 3621Proton acceptor By similarity
Binding sitei362 – 3621Glyoxylate By similarity
Metal bindingi453 – 4531Magnesium By similarity
Binding sitei453 – 4531Glyoxylate By similarity
Metal bindingi481 – 4811Magnesium By similarity
Binding sitei562 – 5621Acetyl-CoA; via carbonyl oxygen By similarity
Active sitei653 – 6531Proton donor By similarity

GO - Molecular functioni

  1. malate synthase activity Source: UniProtKB-HAMAP
  2. metal ion binding Source: UniProtKB-KW
Complete GO annotation...

GO - Biological processi

  1. glyoxylate cycle Source: UniProtKB-HAMAP
  2. tricarboxylic acid cycle Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCEFF196164:GJW8-2270-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase G (EC:2.3.3.9)
Gene namesi
Name:glcB
Synonyms:masZ
Ordered Locus Names:CE2231
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
ProteomesiUP000001409: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 748748Malate synthase GUniRule annotation
PRO_0000166884Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei639 – 6391Cysteine sulfenic acid (-SOH) By similarity

Keywords - PTMi

Oxidation

Proteomic databases

PRIDEiQ8FNB3.

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi196164.CE2231.

Structurei

3D structure databases

ProteinModelPortaliQ8FNB3.
SMRiQ8FNB3. Positions 26-742.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1492Acetyl-CoA binding By similarity
Regioni478 – 4814Glyoxylate binding By similarity

Sequence similaritiesi

Phylogenomic databases

HOGENOMiHOG000220740.
KOiK01638.
OMAiSTIMDFE.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FNB3-1 [UniParc]FASTAAdd to Basket

« Hide

MPAHTRSLAS KELCMNHQQI DAADKTERVT VGGMQVAKVL RDFLTESVLP    50
RVGVDAERFW NGFGDIVRDM TPRNRELLAR RDELQAQLDE YYRENPGKPD 100
PEKYEAFLRE IGYLVDEPAP AEIRTQNIDS EIATTAGPQL VVPILNARFA 150
LNAANARWGS LYDALYGTNA IPDEDGAERG AEYNPVRGQK VIQWGRDFLD 200
AVLPLDGASH ADVEKYNITD GKLAAHVNDG IYRLKDRDAY LGFTGYFEDP 250
TSILLQNNGL HIELQIDPTH PIGKEDKTGL KDIILESAIT TIMDFEDSVA 300
AVDAEDKTLG YRNWFLLNTG ELTEEVAKGD RTFTRKLNDD RVFIGKNGAE 350
LTLHGRSLLF VRNVGHLMTN PAILVDGEEI YEGIMDAIIT TVCAIPGIAP 400
QNKKKNSRKG SIYIVKPKQH GPEEVAFTNE LFARVEDLLD LPRHTLKVGV 450
MDEERRTSVN LDACIMEVAD RLAFINTGFL DRTGDEIHTS MEAGAMVRKA 500
DMQTAPWKQA YEDNNVDAGI QRGLPGKAQI GKGMWAMTEL MGEMLEKKIG 550
QLREGANTAW VPSPTGATLH ATHYHRVDVF KVQDELRAAG RRDSLGKILD 600
VPVAPDTNWT DAEKREELDN NCQSILGYVV RWVEQGVGCS KVPDIHDIDL 650
MEDRATLRIS SQILANWLRH GVVTEEQVIE SLERMAVVVD EQNAGDPNYL 700
NMAPNFTESV AFQAARDLIL KGTESPAGYT EPILHARRRE FKELHGIK 748
Length:748
Mass (Da):83,491
Last modified:March 1, 2003 - v1
Checksum:iF9550473EC4E9A09
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000035 Genomic DNA. Translation: BAC19041.1.
RefSeqiNP_738841.1. NC_004369.1.
WP_011075828.1. NC_004369.1.

Genome annotation databases

EnsemblBacteriaiBAC19041; BAC19041; BAC19041.
GeneIDi1032082.
KEGGicef:CE2231.
PATRICi21490591. VBICorEff9312_2207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
BA000035 Genomic DNA. Translation: BAC19041.1 .
RefSeqi NP_738841.1. NC_004369.1.
WP_011075828.1. NC_004369.1.

3D structure databases

ProteinModelPortali Q8FNB3.
SMRi Q8FNB3. Positions 26-742.
ModBasei Search...

Protein-protein interaction databases

STRINGi 196164.CE2231.

Proteomic databases

PRIDEi Q8FNB3.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAC19041 ; BAC19041 ; BAC19041 .
GeneIDi 1032082.
KEGGi cef:CE2231.
PATRICi 21490591. VBICorEff9312_2207.

Phylogenomic databases

HOGENOMi HOG000220740.
KOi K01638.
OMAi STIMDFE.
OrthoDBi EOG6HJ286.

Enzyme and pathway databases

UniPathwayi UPA00703 ; UER00720 .
BioCyci CEFF196164:GJW8-2270-MONOMER.

Family and domain databases

Gene3Di 2.170.170.11. 2 hits.
HAMAPi MF_00641. Malate_synth_G.
InterProi IPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view ]
Pfami PF01274. Malate_synthase. 1 hit.
[Graphical view ]
SUPFAMi SSF51645. SSF51645. 1 hit.
TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
    Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
    Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Entry informationi

Entry nameiMASZ_COREF
AccessioniPrimary (citable) accession number: Q8FNB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2003
Last modified: September 3, 2014
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi