Q8FNB3 (MASZ_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Malate synthase G
EC=2.3.3.9

Gene names

Name:	glcB
Synonyms:	masZ
Ordered Locus Names:	CE2231

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	748 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA. HAMAP MF_00641
Pathway	Carbohydrate metabolism; glyoxylate cycle; (S)-malate from isocitrate: step 2/2. HAMAP MF_00641
Subunit structure	Monomer By similarity. HAMAP MF_00641
Subcellular location	Cytoplasm By similarity HAMAP MF_00641.
Sequence similarities	Belongs to the malate synthase family. GlcB subfamily.

Ontologies

Keywords
Biological process	Glyoxylate bypass Tricarboxylic acid cycle
Cellular component	Cytoplasm
Molecular function	Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glyoxylate cycle Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	malate synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 748	748	Malate synthase G HAMAP MF_00641		PRO_0000166884
Sites
	Active site	362	1	Proton acceptor By similarity
	Active site	653	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FNB3 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F9550473EC4E9A09	FASTA	748	83,491
	10 20 30 40 50 60 MPAHTRSLAS KELCMNHQQI DAADKTERVT VGGMQVAKVL RDFLTESVLP RVGVDAERFW 70 80 90 100 110 120 NGFGDIVRDM TPRNRELLAR RDELQAQLDE YYRENPGKPD PEKYEAFLRE IGYLVDEPAP 130 140 150 160 170 180 AEIRTQNIDS EIATTAGPQL VVPILNARFA LNAANARWGS LYDALYGTNA IPDEDGAERG 190 200 210 220 230 240 AEYNPVRGQK VIQWGRDFLD AVLPLDGASH ADVEKYNITD GKLAAHVNDG IYRLKDRDAY 250 260 270 280 290 300 LGFTGYFEDP TSILLQNNGL HIELQIDPTH PIGKEDKTGL KDIILESAIT TIMDFEDSVA 310 320 330 340 350 360 AVDAEDKTLG YRNWFLLNTG ELTEEVAKGD RTFTRKLNDD RVFIGKNGAE LTLHGRSLLF 370 380 390 400 410 420 VRNVGHLMTN PAILVDGEEI YEGIMDAIIT TVCAIPGIAP QNKKKNSRKG SIYIVKPKQH 430 440 450 460 470 480 GPEEVAFTNE LFARVEDLLD LPRHTLKVGV MDEERRTSVN LDACIMEVAD RLAFINTGFL 490 500 510 520 530 540 DRTGDEIHTS MEAGAMVRKA DMQTAPWKQA YEDNNVDAGI QRGLPGKAQI GKGMWAMTEL 550 560 570 580 590 600 MGEMLEKKIG QLREGANTAW VPSPTGATLH ATHYHRVDVF KVQDELRAAG RRDSLGKILD 610 620 630 640 650 660 VPVAPDTNWT DAEKREELDN NCQSILGYVV RWVEQGVGCS KVPDIHDIDL MEDRATLRIS 670 680 690 700 710 720 SQILANWLRH GVVTEEQVIE SLERMAVVVD EQNAGDPNYL NMAPNFTESV AFQAARDLIL 730 740 KGTESPAGYT EPILHARRRE FKELHGIK

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC19041.1.
RefSeq	NP_738841.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FNB3.
SMR	Q8FNB3. Positions 26-742.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1032082.
GenomeReviews	Gene locus CE2231 in contig BA000035_GR.
KEGG	cef:CE2231.
NMPDR	fig|196164.1.peg.2231.
PATRIC	21490591. VBICorEff9312_2207.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG350005.
OMA	PKMHGPD.
PhylomeDB	Q8FNB3.
ProtClustDB	PRK02999.
Enzyme and pathway databases
BioCyc	CEFF196164:CE2231-MONOMER.
Family and domain databases
HAMAP	MF_00641. Malate_synth_G. [Tree]
InterPro	IPR011076. Malate_synth-like. IPR023310. Malate_synth_G_beta_sub_dom. IPR001465. Malate_synthase. IPR006253. Malate_synthG. [Graphical view]
Gene3D	G3DSA:2.170.170.11. Malate_synth_G_beta_sub_dom. 2 hits.
KO	K01638.
PANTHER	PTHR21631:SF1. Malate_synthase. 1 hit.
Pfam	PF01274. Malate_synthase. 1 hit. [Graphical view]
SUPFAM	SSF51645. Malat_synth_like. 1 hit.
TIGRFAMs	TIGR01345. Malate_syn_G. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MASZ_COREF

Accession

Primary (citable) accession number: Q8FNB3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program