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Q8FNB3

- MASZ_COREF

UniProt

Q8FNB3 - MASZ_COREF

Protein

Malate synthase G

Gene

glcB

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 78 (01 Oct 2014)
      Sequence version 1 (01 Mar 2003)
      Previous versions | rss
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    Functioni

    Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

    Catalytic activityi

    Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei141 – 1411Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Binding sitei298 – 2981Acetyl-CoAUniRule annotation
    Binding sitei335 – 3351Acetyl-CoAUniRule annotation
    Active sitei362 – 3621Proton acceptorUniRule annotation
    Binding sitei362 – 3621GlyoxylateUniRule annotation
    Metal bindingi453 – 4531MagnesiumUniRule annotation
    Binding sitei453 – 4531GlyoxylateUniRule annotation
    Metal bindingi481 – 4811MagnesiumUniRule annotation
    Binding sitei562 – 5621Acetyl-CoA; via carbonyl oxygenUniRule annotation
    Active sitei653 – 6531Proton donorUniRule annotation

    GO - Molecular functioni

    1. malate synthase activity Source: UniProtKB-HAMAP
    2. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. glyoxylate cycle Source: UniProtKB-HAMAP
    2. tricarboxylic acid cycle Source: UniProtKB-KW

    Keywords - Molecular functioni

    Transferase

    Keywords - Biological processi

    Glyoxylate bypass, Tricarboxylic acid cycle

    Keywords - Ligandi

    Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciCEFF196164:GJW8-2270-MONOMER.
    UniPathwayiUPA00703; UER00720.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
    Gene namesi
    Name:glcBUniRule annotation
    Synonyms:masZ
    Ordered Locus Names:CE2231
    OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
    Taxonomic identifieri196164 [NCBI]
    Taxonomic lineageiBacteriaActinobacteriaActinobacteridaeActinomycetalesCorynebacterineaeCorynebacteriaceaeCorynebacterium
    ProteomesiUP000001409: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 748748Malate synthase GPRO_0000166884Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei639 – 6391Cysteine sulfenic acid (-SOH)UniRule annotation

    Keywords - PTMi

    Oxidation

    Proteomic databases

    PRIDEiQ8FNB3.

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi196164.CE2231.

    Structurei

    3D structure databases

    ProteinModelPortaliQ8FNB3.
    SMRiQ8FNB3. Positions 26-742.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni148 – 1492Acetyl-CoA bindingUniRule annotation
    Regioni478 – 4814Glyoxylate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

    Phylogenomic databases

    HOGENOMiHOG000220740.
    KOiK01638.
    OMAiSTIMDFE.
    OrthoDBiEOG6HJ286.

    Family and domain databases

    Gene3Di2.170.170.11. 2 hits.
    HAMAPiMF_00641. Malate_synth_G.
    InterProiIPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view]
    PfamiPF01274. Malate_synthase. 1 hit.
    [Graphical view]
    SUPFAMiSSF51645. SSF51645. 1 hit.
    TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q8FNB3-1 [UniParc]FASTAAdd to Basket

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    MPAHTRSLAS KELCMNHQQI DAADKTERVT VGGMQVAKVL RDFLTESVLP    50
    RVGVDAERFW NGFGDIVRDM TPRNRELLAR RDELQAQLDE YYRENPGKPD 100
    PEKYEAFLRE IGYLVDEPAP AEIRTQNIDS EIATTAGPQL VVPILNARFA 150
    LNAANARWGS LYDALYGTNA IPDEDGAERG AEYNPVRGQK VIQWGRDFLD 200
    AVLPLDGASH ADVEKYNITD GKLAAHVNDG IYRLKDRDAY LGFTGYFEDP 250
    TSILLQNNGL HIELQIDPTH PIGKEDKTGL KDIILESAIT TIMDFEDSVA 300
    AVDAEDKTLG YRNWFLLNTG ELTEEVAKGD RTFTRKLNDD RVFIGKNGAE 350
    LTLHGRSLLF VRNVGHLMTN PAILVDGEEI YEGIMDAIIT TVCAIPGIAP 400
    QNKKKNSRKG SIYIVKPKQH GPEEVAFTNE LFARVEDLLD LPRHTLKVGV 450
    MDEERRTSVN LDACIMEVAD RLAFINTGFL DRTGDEIHTS MEAGAMVRKA 500
    DMQTAPWKQA YEDNNVDAGI QRGLPGKAQI GKGMWAMTEL MGEMLEKKIG 550
    QLREGANTAW VPSPTGATLH ATHYHRVDVF KVQDELRAAG RRDSLGKILD 600
    VPVAPDTNWT DAEKREELDN NCQSILGYVV RWVEQGVGCS KVPDIHDIDL 650
    MEDRATLRIS SQILANWLRH GVVTEEQVIE SLERMAVVVD EQNAGDPNYL 700
    NMAPNFTESV AFQAARDLIL KGTESPAGYT EPILHARRRE FKELHGIK 748
    Length:748
    Mass (Da):83,491
    Last modified:March 1, 2003 - v1
    Checksum:iF9550473EC4E9A09
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000035 Genomic DNA. Translation: BAC19041.1.
    RefSeqiNP_738841.1. NC_004369.1.
    WP_011075828.1. NC_004369.1.

    Genome annotation databases

    EnsemblBacteriaiBAC19041; BAC19041; BAC19041.
    GeneIDi1032082.
    KEGGicef:CE2231.
    PATRICi21490591. VBICorEff9312_2207.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    BA000035 Genomic DNA. Translation: BAC19041.1 .
    RefSeqi NP_738841.1. NC_004369.1.
    WP_011075828.1. NC_004369.1.

    3D structure databases

    ProteinModelPortali Q8FNB3.
    SMRi Q8FNB3. Positions 26-742.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 196164.CE2231.

    Proteomic databases

    PRIDEi Q8FNB3.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai BAC19041 ; BAC19041 ; BAC19041 .
    GeneIDi 1032082.
    KEGGi cef:CE2231.
    PATRICi 21490591. VBICorEff9312_2207.

    Phylogenomic databases

    HOGENOMi HOG000220740.
    KOi K01638.
    OMAi STIMDFE.
    OrthoDBi EOG6HJ286.

    Enzyme and pathway databases

    UniPathwayi UPA00703 ; UER00720 .
    BioCyci CEFF196164:GJW8-2270-MONOMER.

    Family and domain databases

    Gene3Di 2.170.170.11. 2 hits.
    HAMAPi MF_00641. Malate_synth_G.
    InterProi IPR011076. Malate_synth-like.
    IPR023310. Malate_synth_G_beta_sub_dom.
    IPR001465. Malate_synthase.
    IPR006253. Malate_synthG.
    [Graphical view ]
    Pfami PF01274. Malate_synthase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51645. SSF51645. 1 hit.
    TIGRFAMsi TIGR01345. malate_syn_G. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
      Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
      Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

    Entry informationi

    Entry nameiMASZ_COREF
    AccessioniPrimary (citable) accession number: Q8FNB3
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: April 30, 2003
    Last sequence update: March 1, 2003
    Last modified: October 1, 2014
    This is version 78 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3