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Protein

Malate synthase G

Gene

glcB

Organism
Corynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Involved in the glycolate utilization. Catalyzes the condensation and subsequent hydrolysis of acetyl-coenzyme A (acetyl-CoA) and glyoxylate to form malate and CoA.UniRule annotation

Catalytic activityi

Acetyl-CoA + H2O + glyoxylate = (S)-malate + CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Pathway:iglyoxylate cycle

This protein is involved in step 2 of the subpathway that synthesizes (S)-malate from isocitrate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Isocitrate lyase (aceA)
  2. Malate synthase G (glcB)
This subpathway is part of the pathway glyoxylate cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (S)-malate from isocitrate, the pathway glyoxylate cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei141 – 1411Acetyl-CoA; via carbonyl oxygenUniRule annotation
Binding sitei298 – 2981Acetyl-CoAUniRule annotation
Binding sitei335 – 3351Acetyl-CoAUniRule annotation
Active sitei362 – 3621Proton acceptorUniRule annotation
Binding sitei362 – 3621GlyoxylateUniRule annotation
Metal bindingi453 – 4531MagnesiumUniRule annotation
Binding sitei453 – 4531GlyoxylateUniRule annotation
Metal bindingi481 – 4811MagnesiumUniRule annotation
Binding sitei562 – 5621Acetyl-CoA; via carbonyl oxygenUniRule annotation
Active sitei653 – 6531Proton donorUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Glyoxylate bypass, Tricarboxylic acid cycle

Keywords - Ligandi

Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciCEFF196164:GJW8-2270-MONOMER.
UniPathwayiUPA00703; UER00720.

Names & Taxonomyi

Protein namesi
Recommended name:
Malate synthase GUniRule annotation (EC:2.3.3.9UniRule annotation)
Gene namesi
Name:glcBUniRule annotation
Synonyms:masZ
Ordered Locus Names:CE2231
OrganismiCorynebacterium efficiens (strain DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395)
Taxonomic identifieri196164 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesCorynebacteriaceaeCorynebacterium
ProteomesiUP000001409 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 748748Malate synthase GPRO_0000166884Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei639 – 6391Cysteine sulfenic acid (-SOH)UniRule annotation

Keywords - PTMi

Oxidation

Proteomic databases

PRIDEiQ8FNB3.

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi196164.HMPREF0290_2269.

Structurei

3D structure databases

ProteinModelPortaliQ8FNB3.
SMRiQ8FNB3. Positions 26-742.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni148 – 1492Acetyl-CoA bindingUniRule annotation
Regioni478 – 4814Glyoxylate bindingUniRule annotation

Sequence similaritiesi

Belongs to the malate synthase family. GlcB subfamily.UniRule annotation

Phylogenomic databases

HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.

Sequencei

Sequence statusi: Complete.

Q8FNB3-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPAHTRSLAS KELCMNHQQI DAADKTERVT VGGMQVAKVL RDFLTESVLP
60 70 80 90 100
RVGVDAERFW NGFGDIVRDM TPRNRELLAR RDELQAQLDE YYRENPGKPD
110 120 130 140 150
PEKYEAFLRE IGYLVDEPAP AEIRTQNIDS EIATTAGPQL VVPILNARFA
160 170 180 190 200
LNAANARWGS LYDALYGTNA IPDEDGAERG AEYNPVRGQK VIQWGRDFLD
210 220 230 240 250
AVLPLDGASH ADVEKYNITD GKLAAHVNDG IYRLKDRDAY LGFTGYFEDP
260 270 280 290 300
TSILLQNNGL HIELQIDPTH PIGKEDKTGL KDIILESAIT TIMDFEDSVA
310 320 330 340 350
AVDAEDKTLG YRNWFLLNTG ELTEEVAKGD RTFTRKLNDD RVFIGKNGAE
360 370 380 390 400
LTLHGRSLLF VRNVGHLMTN PAILVDGEEI YEGIMDAIIT TVCAIPGIAP
410 420 430 440 450
QNKKKNSRKG SIYIVKPKQH GPEEVAFTNE LFARVEDLLD LPRHTLKVGV
460 470 480 490 500
MDEERRTSVN LDACIMEVAD RLAFINTGFL DRTGDEIHTS MEAGAMVRKA
510 520 530 540 550
DMQTAPWKQA YEDNNVDAGI QRGLPGKAQI GKGMWAMTEL MGEMLEKKIG
560 570 580 590 600
QLREGANTAW VPSPTGATLH ATHYHRVDVF KVQDELRAAG RRDSLGKILD
610 620 630 640 650
VPVAPDTNWT DAEKREELDN NCQSILGYVV RWVEQGVGCS KVPDIHDIDL
660 670 680 690 700
MEDRATLRIS SQILANWLRH GVVTEEQVIE SLERMAVVVD EQNAGDPNYL
710 720 730 740
NMAPNFTESV AFQAARDLIL KGTESPAGYT EPILHARRRE FKELHGIK
Length:748
Mass (Da):83,491
Last modified:March 1, 2003 - v1
Checksum:iF9550473EC4E9A09
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC19041.1.

Genome annotation databases

EnsemblBacteriaiBAC19041; BAC19041; BAC19041.
KEGGicef:CE2231.
PATRICi21490591. VBICorEff9312_2207.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BA000035 Genomic DNA. Translation: BAC19041.1.

3D structure databases

ProteinModelPortaliQ8FNB3.
SMRiQ8FNB3. Positions 26-742.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi196164.HMPREF0290_2269.

Proteomic databases

PRIDEiQ8FNB3.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiBAC19041; BAC19041; BAC19041.
KEGGicef:CE2231.
PATRICi21490591. VBICorEff9312_2207.

Phylogenomic databases

HOGENOMiHOG000220740.
KOiK01638.
OMAiPKMHGPD.
OrthoDBiEOG6HJ286.

Enzyme and pathway databases

UniPathwayiUPA00703; UER00720.
BioCyciCEFF196164:GJW8-2270-MONOMER.

Family and domain databases

Gene3Di2.170.170.11. 2 hits.
HAMAPiMF_00641. Malate_synth_G.
InterProiIPR011076. Malate_synth-like.
IPR023310. Malate_synth_G_beta_sub_dom.
IPR001465. Malate_synthase.
IPR006253. Malate_synthG.
[Graphical view]
PfamiPF01274. Malate_synthase. 1 hit.
[Graphical view]
SUPFAMiSSF51645. SSF51645. 1 hit.
TIGRFAMsiTIGR01345. malate_syn_G. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens."
    Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T.
    Genome Res. 13:1572-1579(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Entry informationi

Entry nameiMASZ_COREF
AccessioniPrimary (citable) accession number: Q8FNB3
Entry historyi
Integrated into UniProtKB/Swiss-Prot: April 30, 2003
Last sequence update: March 1, 2003
Last modified: July 22, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.