Q8FMU9 (NTPA_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Non-canonical purine NTP pyrophosphatase
EC=3.6.1.19

Alternative name(s):
Non-standard purine NTP pyrophosphatase
Nucleoside-triphosphate diphosphatase
Nucleoside-triphosphate pyrophosphatase
Short name=NTPase

Gene names

Ordered Locus Names:

CE2400

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	207 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Pyrophosphatase that hydrolyzes non-canonical purine nucleotides such as XTP and ITP/dITP to their respective monophosphate derivatives. Might exclude non-canonical purines from DNA precursor pool, thus preventing their incorporation into DNA and avoiding chromosomal lesions By similarity. HAMAP MF_01405
Catalytic activity	A nucleoside triphosphate + H2O = a nucleotide + diphosphate. HAMAP MF_01405
Cofactor	Binds 1 divalent metal cation ion per subunit; can use either magnesium or manganese By similarity.
Subunit structure	Homodimer By similarity. HAMAP MF_01405
Sequence similarities	Belongs to the HAM1 NTPase family.
Sequence caution	The sequence BAC19210.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Biological process	Nucleotide metabolism
Ligand	Magnesium Manganese Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	nucleotide metabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleoside-triphosphatase activity Inferred from electronic annotation. Source: InterPro nucleoside-triphosphate diphosphatase activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 207	207	Non-canonical purine NTP pyrophosphatase HAMAP MF_01405		PRO_0000178158
Regions
	Region	7 – 12	6	Substrate binding By similarity
	Region	72 – 73	2	Substrate binding By similarity
Sites
	Metal binding	72	1	Magnesium or manganese By similarity
	Binding site	158	1	Substrate By similarity
	Binding site	184	1	Substrate By similarity
	Binding site	190	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FMU9 [UniParc]. Last modified June 28, 2011. Version 2. Checksum: 52EEAC3EECDCAA4D	FASTA	207	22,088
	10 20 30 40 50 60 MKLLVASNNA KKLGELQRIL DQAGIENVEL LALADVPSYP EPVEDGRTFT ENALIKARAG 70 80 90 100 110 120 ASNTGLITLA DDSGLEVDAL NGMPGVLSAR WAGKHGNDQA NNDLLLAQIA DIPEEHRGAA 130 140 150 160 170 180 FVSVCAIVTP DGREFVEEGR WHGTLLREPV GTNGFGYDPL FVPMEESLIE GRDRSSAQLT 190 200 AQEKDALSHR GKALRALVPA IAELAGQ

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC19210.1. Different initiation.
RefSeq	NP_739010.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FMU9.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1034567.
GenomeReviews	Gene locus CE2400 in contig BA000035_GR.
KEGG	cef:CE2400.
NMPDR	fig|196164.1.peg.2400.
PATRIC	21490931. VBICorEff9312_2370.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG697237.
OMA	GVYTADW.
ProtClustDB	PRK00120.
Enzyme and pathway databases
BioCyc	CEFF196164:CE2400-MONOMER.
Family and domain databases
HAMAP	MF_01405. Non_canon_purine_NTPase. [Tree]
InterPro	IPR002637. Ham1p-like. IPR020922. Nucleoside-triphosphatase. [Graphical view]
KO	K02428.
PANTHER	PTHR11067. Ham1p_like. 1 hit.
Pfam	PF01725. Ham1p_like. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NTPA_COREF

Accession

Primary (citable) accession number: Q8FMU9

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 26, 2005
Last sequence update:	June 28, 2011
Last modified:	January 25, 2012
This is version 47 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program