Q8FMS2 (NADE_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	CE2428

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	277 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.
Sequence caution	The sequence BAC19238.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 277	277	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_0000152164
Regions
	Nucleotide binding	46 – 53	8	ATP By similarity
Sites
	Active site	48	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FMS2 [UniParc]. Last modified April 13, 2004. Version 2. Checksum: 1C77DE763ED2DE97	FASTA	277	30,229
	10 20 30 40 50 60 MTDTRDHIIS QLSVVPSIEP AAEVEARVQF LVDYLRVSHA RGYVLGISGG QDSTIAGRLA 70 80 90 100 110 120 QLAVERIRRE DGSDHQFVGV RLPHGLQADE DDALVALDFI QPDRSISINI REATDVLSAA 130 140 150 160 170 180 TAAALGIDEL GDFNKGNVKA RQRMVAQYAI AGELGLLVVG TDHAAENVTG FFTKYGDGAA 190 200 210 220 230 240 DVLPLAGLTK RQGALLLQHL GAPESTWTKV PTADLEEDRP ALPDEEALGV TYREIDTYLE 250 260 270 NSGEVSPEAA ARIEHLWKVG QHKRHMPVTP QDQWWRQ

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC19238.1. Different initiation.
RefSeq	NP_739038.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FMS2.
SMR	Q8FMS2. Positions 6-276.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1034546.
GenomeReviews	Gene locus CE2428 in contig BA000035_GR.
KEGG	cef:CE2428.
NMPDR	fig|196164.1.peg.2428.
PATRIC	21490985. VBICorEff9312_2397.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG351567.
PhylomeDB	Q8FMS2.
ProtClustDB	PRK00768.
Enzyme and pathway databases
BioCyc	CEFF196164:CE2428-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_COREF

Accession

Primary (citable) accession number: Q8FMS2

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 13, 2004
Last sequence update:	April 13, 2004
Last modified:	January 25, 2012
This is version 44 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program