Reviewed, UniProtKB/Swiss-Prot Q8FMR1 (ILVD2_COREF)

Names and origin

Protein names

Recommended name:
    Dihydroxy-acid dehydratase 2
      Short name=DAD 2
    EC=4.2.1.9

Gene names

Name:	ilvD2
Ordered Locus Names:	CE2439

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential. HAMAP MF_00012
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the ilvD/edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isoleucine biosynthetic process Inferred from electronic annotation. Source: HAMAP valine biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 567	567	Dihydroxy-acid dehydratase 2 HAMAP MF_00012		PRO_0000103462
Sites
	Metal binding	129	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	206	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8FMR1-1 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 4C3CE912E05677B2	FASTA	567	59,729
	10 20 30 40 50 60 MTNDHDIDIK PRSRDVTDGL ERTAARGMLR AVGMGDDDWE KPQIGVASSW NEITPCNLTL 70 80 90 100 110 120 KKLAGFAKEG VHEAGGYPLE FGTISVSDGI SMGHEGMHYS LVSREVITDS VETVMSAERL 130 140 150 160 170 180 DGSVLLAGCD KSIPGMLMAA ARLNLSSVFL YNGSTMPGTA KMSDGTEREV TLIDAFEAVG 190 200 210 220 230 240 ACRAGTMSRE DVDAIERSVC PGEGACGGMY TANTMASAAE AMGMSLPGSA APPAIHQDRT 250 260 270 280 290 300 LYARRSGEAV VELLRRGIRA RDIITRESLL NAVAVVMALG GSTNAVLHLM AIAHEAEVDL 310 320 330 340 350 360 TLEDFNAVGD KVPHLGDLKP FGRYVMNDVF KIGGIPVVMK ALLDAGLING DCLTITGRTV 370 380 390 400 410 420 AENLQGINPP DPDGQILRAI DNPIHKTGGL TILHGSLAPG GAVVKTAGFD TERFEGTARV 430 440 450 460 470 480 FNQEAPAMDA VLNGELKAGD VVIIRYEGPK GGPGMREMLA ITGAIKGAGI GKEVLLITDG 490 500 510 520 530 540 RFSGGSTGLC IGHVAPEAVD GGPIALVEDG DPILVDISQR RIDLLVDESI LEERRKTLQH 550 560 PENPRLHGVL GKYAKLVQSA SMGAVCF

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC19249.1.
RefSeq	NP_739049.1.
3D structure databases
SMR	Q8FMR1. Positions 72-567.
ModBase	Search...
Genome annotation databases
GeneID	1034515.
GenomeReviews	Gene locus CE2439 in contig BA000035_GR.
KEGG	cef:CE2439.
NMPDR	fig|196164.1.peg.2439.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG671001.
OMA	NMPGAMI.
PhylomeDB	Q8FMR1.
Enzyme and pathway databases
BioCyc	CEFF196164:CE2439-MONOMER.
BRENDA	4.2.1.9. 277326.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
TIGRFAMs	TIGR00110. ilvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD2_COREF

Accession

Primary (citable) accession number: Q8FMR1

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 15, 2003
Last sequence update:	March 1, 2003
Last modified:	February 9, 2010
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)