Q8FMM3 (PURL_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine synthase 2
EC=6.3.5.3

Alternative name(s):
Phosphoribosylformylglycinamidine synthase II
Short name=FGAM synthase II

Gene names

Name:	purL
Ordered Locus Names:	CE2480

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	763 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide + L-glutamine + H2O = ADP + phosphate + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine + L-glutamate. HAMAP MF_00420
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 1/2. HAMAP MF_00420
Subunit structure	Heterodimer of two subunits, PurQ and PurL.
Subcellular location	Cytoplasm By similarity HAMAP MF_00420.
Sequence similarities	Belongs to the FGAMS family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 763	763	Phosphoribosylformylglycinamidine synthase 2 HAMAP MF_00420		PRO_0000100451
Regions
	Nucleotide binding	120 – 131	12	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q8FMM3 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: F50068E33FC109B8	FASTA	763	80,705
	10 20 30 40 50 60 MSTIFNDTVE AAAENPDLEQ PYAELGLKDD EYARIKEILG RRPTDAELTV YSVMWSEHCS 70 80 90 100 110 120 YKSSKVHLRY FGETTTPEMA EKILAGIGEN AGVVDIGDGN AVTFRVESHN HPSFVEPHQG 130 140 150 160 170 180 AATGIGGIVR DIMAMGARPI AVMDQLRFGA MDNPDTQRVL PGVVSGISHY GNCLGLPNIG 190 200 210 220 230 240 GETVFDDSYA GNPLVNALAV GTLKVDDLKL AFASGAGNKV MLFGSRTGLD GIGGVSVLGS 250 260 270 280 290 300 ASFEEGEERK LPAVQVGDPF AEKVLIECCL ELYQAGVVVG IQDLGGGGLA CATSELAAAG 310 320 330 340 350 360 DGGMRVNLDA VPLRAEKMSA AEILASESQE RMCAVVTPEN VEKFKQICAK WDVTVAEIGE 370 380 390 400 410 420 VTDEKDRYLV VHNGQTVIDA PASTIDEGPV YNRPVERPAN QDEIQTLGGL ERPDTAEGIR 430 440 450 460 470 480 DAWFKLVSSP ALCSRAFITE QYDRYVRGNT VQAKDANAGV LRIDEETNRG VAISADASGR 490 500 510 520 530 540 YTRLDPRTGA RLALAEAYRN VVSTGARPVA VTNCLNFGSP ENAGVMWQFK EAVHGLADGA 550 560 570 580 590 600 KEMGIPVSGG NVSFYNQTGD EPILPTPVVG VLGVLDDVEK TIGNRLPAGE QELYLLGETL 610 620 630 640 650 660 DEFGGSIWQQ VSGAGLSGLP PQIDLANEQR LADLLVPAAD LVGAAHDLSE GGLGQTLAEL 670 680 690 700 710 720 AIHAGRGLDV DLTQVHEDLF TALFSESASR IVVATTRGDE LVARAQELGV PVTRLGVTND 730 740 750 760 SGVITVRGGD LSVEVSVEEL HDAWSNTLPE AFGHAVGANS VVE

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC19290.1.
RefSeq	NP_739090.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FMM3.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1032100.
GenomeReviews	Gene locus CE2480 in contig BA000035_GR.
KEGG	cef:CE2480.
NMPDR	fig|196164.1.peg.2480.
PATRIC	21491107. VBICorEff9312_2448.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG311214.
OMA	YGNSFGV.
PhylomeDB	Q8FMM3.
ProtClustDB	PRK01213.
Enzyme and pathway databases
BioCyc	CEFF196164:CE2480-MONOMER.
Family and domain databases
HAMAP	MF_00420. PurL_2. [Tree]
InterPro	IPR000728. AIR_synth. IPR010918. AIR_synth_C. IPR010074. PRibForGlyAmidine_synth_II. IPR016188. PurM_N-like. [Graphical view]
KO	K01952.
Pfam	PF00586. AIRS. 2 hits. PF02769. AIRS_C. 2 hits. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 2 hits. SSF55326. PurM_N-like. 2 hits.
TIGRFAMs	TIGR01736. FGAM_synth_II. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PURL_COREF

Accession

Primary (citable) accession number: Q8FMM3

Entry history

Integrated into UniProtKB/Swiss-Prot:	June 7, 2005
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program