Q8FMI8 (SYC_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cysteine--tRNA ligase
EC=6.1.1.16

Alternative name(s):
Cysteinyl-tRNA synthetase
Short name=CysRS

Gene names

Name:	cysS
Synonyms:	cysS1
Ordered Locus Names:	CE2516

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	459 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-cysteine + tRNA(Cys) = AMP + diphosphate + L-cysteinyl-tRNA(Cys). HAMAP MF_00041
Cofactor	Binds 1 zinc ion per subunit By similarity. HAMAP MF_00041
Subunit structure	Monomer By similarity. HAMAP MF_00041
Subcellular location	Cytoplasm HAMAP MF_00041.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.
Sequence caution	The sequence BAC19326.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cysteinyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW cysteine-tRNA ligase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 459	459	Cysteine--tRNA ligase HAMAP MF_00041		PRO_0000159386
Regions
	Motif	31 – 41	11	"HIGH" region HAMAP MF_00041
	Motif	268 – 272	5	"KMSKS" region HAMAP MF_00041
Sites
	Metal binding	29	1	Zinc By similarity
	Metal binding	212	1	Zinc By similarity
	Metal binding	237	1	Zinc By similarity
	Metal binding	241	1	Zinc By similarity
	Binding site	271	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FMI8 [UniParc]. Last modified April 30, 2003. Version 2. Checksum: 145FA78B3DED589C	FASTA	459	50,836
	10 20 30 40 50 60 MTLRIFDTAT RTLREFIPLK PGHASVYLCG ATPQAQPHIG HVRSGVAFDI LRRWLLAKGL 70 80 90 100 110 120 DVAFVRNVTD IDDKILTKAA EHGRPWWEWV STYEREFTWA YNTLGVLPPS VEPRATGHVT 130 140 150 160 170 180 QMVQYMQRLI DNGFAYEVNG SVYFDVTAWV AAEGSDYGSL SGNRVEDMEQ GETDNFGKRG 190 200 210 220 230 240 PQDFALWKAA KPGEPSWPTP WGEGRPGWHL ECSAMATYYL GAEFDIHCGG LDLQFPHHEN 250 260 270 280 290 300 EIAQAHAAGD GFARYWMHNH WVTMAGEKMS KSLGNVLSVP NMLEKVRPVE LRYYLGSAHY 310 320 330 340 350 360 RSVLEYSEGA LTEAAAGYRR IEAFVDRVGE VEVGTWTAGF EAAMDEDLAV PKALAEIHNA 370 380 390 400 410 420 VREGNSALAA GEMEKARELA GAVRAMTGVL GFDPVEWGTQ TDDSAAHEAL EVLVHAELER 430 440 450 RATARAEKDW AVADEVRDRL AAAGIEVVDT ADGVSWKLQ

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC19326.1. Different initiation.
RefSeq	NP_739126.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FMI8.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1034358.
GenomeReviews	Gene locus CE2516 in contig BA000035_GR.
KEGG	cef:CE2516.
NMPDR	fig|196164.1.peg.2516.
PATRIC	21491181. VBICorEff9312_2484.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG327651.
PhylomeDB	Q8FMI8.
ProtClustDB	PRK00260.
Enzyme and pathway databases
BioCyc	CEFF196164:CE2516-MONOMER.
Family and domain databases
HAMAP	MF_00041. Cys_tRNA_synth. [Tree]
InterPro	IPR015803. Cys-tRNA-synt. IPR015273. Cys-tRNA-synt_Ia_DALR. IPR024909. Cys-tRNA/MSH_ligase. IPR014729. Rossmann-like_a/b/a_fold. IPR009080. tRNAsynth_1a_anticodon-bd. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits.
KO	K01883.
PANTHER	PTHR10890. Cys_tRNA-synt_1a. 1 hit.
Pfam	PF09190. DALR_2. 1 hit. PF01406. tRNA-synt_1e. 1 hit. [Graphical view]
PRINTS	PR00983. TRNASYNTHCYS.
SMART	SM00840. DALR_2. 1 hit. [Graphical view]
SUPFAM	SSF47323. tRNAsyn_1a_bind. 1 hit.
TIGRFAMs	TIGR00435. CysS. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYC_COREF

Accession

Primary (citable) accession number: Q8FMI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 30, 2003
Last sequence update:	April 30, 2003
Last modified:	January 25, 2012
This is version 59 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program