Q8FMF8 (IPYR_COREF) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Inorganic pyrophosphatase
EC=3.6.1.1

Alternative name(s):
Pyrophosphate phospho-hydrolase
Short name=PPase

Gene names

Name:	ppa
Ordered Locus Names:	CE2546

Organism

Corynebacterium efficiens [Complete proteome] [HAMAP]

Taxonomic identifier

152794 [NCBI]

Taxonomic lineage

Bacteria › Actinobacteria › Actinobacteridae › Actinomycetales › Corynebacterineae › Corynebacteriaceae › Corynebacterium

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	Diphosphate + H2O = 2 phosphate. HAMAP MF_00209
Cofactor	Binds 4 magnesium ions per subunit. Other metal ions can support activity, but at a lower rate. Two magnesium ions are required for the activation of the enzyme and are present before substrate binds, two additional magnesium ions form complexes with substrate and product By similarity.
Subcellular location	Cytoplasm By similarity HAMAP MF_00209.
Sequence similarities	Belongs to the PPase family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phosphate-containing compound metabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	inorganic diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 158	158	Inorganic pyrophosphatase HAMAP MF_00209		PRO_0000137493
Sites
	Metal binding	52	1	Magnesium 1 By similarity
	Metal binding	57	1	Magnesium 1 By similarity
	Metal binding	57	1	Magnesium 2 By similarity
	Metal binding	89	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q8FMF8 [UniParc]. Last modified March 1, 2003. Version 1. Checksum: 8997B4E0F6B6DA35	FASTA	158	17,912
	10 20 30 40 50 60 MSVEVTVEIP KGSRNKYEID HETGKVYLDR YLFTPMAYPL DYGFIDHTLG EDGDPMDALV 70 80 90 100 110 120 ILPESVFPNV IVKSRVIGVF KMTDEAGGDD KLLAVLDDPR YDHIQDISDV SDFLKDEIEH 130 140 150 FFVHYKDLEK GKHVDGSGWG DKAEAEQILA DSIERYKA

References

[1]

"Comparative complete genome sequence analysis of the amino acid replacements responsible for the thermostability of Corynebacterium efficiens." Nishio Y., Nakamura Y., Kawarabayasi Y., Usuda Y., Kimura E., Sugimoto S., Matsui K., Yamagishi A., Kikuchi H., Ikeo K., Gojobori T. Genome Res. 13:1572-1579(2003) [PubMed: 12840036] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 44549 / YS-314 / AJ 12310 / JCM 11189 / NBRC 100395.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000035 Genomic DNA. Translation: BAC19356.1.
RefSeq	NP_739156.1. NC_004369.1.
3D structure databases
ProteinModelPortal	Q8FMF8.
SMR	Q8FMF8. Positions 1-158.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1034280.
GenomeReviews	Gene locus CE2546 in contig BA000035_GR.
KEGG	cef:CE2546.
NMPDR	fig|196164.1.peg.2546.
PATRIC	21491243. VBICorEff9312_2515.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	HBG529150.
OMA	YGFFENT.
PhylomeDB	Q8FMF8.
ProtClustDB	CLSK633924.
Enzyme and pathway databases
BioCyc	CEFF196164:CE2546-MONOMER.
Family and domain databases
HAMAP	MF_00209. Inorganic_PPase. [Tree]
InterPro	IPR008162. Pyrophosphatase. [Graphical view]
Gene3D	G3DSA:3.90.80.10. Pyrophosphatase. 1 hit.
KO	K01507.
PANTHER	PTHR10286. Pyrophosphatase. 1 hit.
Pfam	PF00719. Pyrophosphatase. 1 hit. [Graphical view]
SUPFAM	SSF50324. Pyrophosphatase. 1 hit.
PROSITE	PS00387. PPASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

IPYR_COREF

Accession

Primary (citable) accession number: Q8FMF8

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 11, 2003
Last sequence update:	March 1, 2003
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program